JHDM1D (KDM7A) (NM_030647) Human Tagged ORF Clone

CAT＃: RG218944

TrueORF^®

JHDM1D (tGFP-tagged) - Human jumonji C domain containing histone demethylase 1 homolog D (S. cerevisiae) (JHDM1D)

ORF Plasmid: DDK tGFP

Datasheet

SDS

Plasmid Map

"NM_030647" in other vectors (6)

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CNY 7,890.00

货期*

4周

规格

Cited in 1 publication.

Product images

经常一起买 (5)

PS100010

pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug

CNY 6,080.00

TA150041

Mouse monoclonal turboGFP antibody, clone OTI2H8

CNY 1,000.00

【特别福利】购一抗正装产品，免费领10ul抗体试用装

CC100003

DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)

CNY 1,280.00

PRIMER

Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each

CNY 480.00

TA372814S

KDM7A rabbit polyclonal antibody

CNY 800.00
CNY 1,280.00

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	TurboGFP
Synonyms	JHDM1D
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RG218944 representing NM_030647 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGGAGCGGCGGCGGCGGTGGCCGCGGGAGCAGCAGCTGGAGCCGCCGCGGCAGCCGTGTCGGTGG CGGCTCCCGGCCGGGCCTCGGCGCCTCCGCCGCCCCCGCCCGTGTACTGTGTGTGCCGGCAGCCGTACGA CGTGAACCGCTTCATGATCGAGTGCGATATCTGCAAGGACTGGTTCCACGGCAGCTGTGTTGGAGTAGAA GAACATCATGCTGTTGACATTGACCTGTATCACTGTCCCAACTGTGCAGTTTTACATGGTTCCTCCTTGA TGAAAAAAAGGAGGAACTGGCACAGACATGACTACACAGAAATTGATGATGGTTCCAAACCAGTGCAAGC TGGAACTAGAACTTTCATTAAGGAATTACGCTCTCGAGTCTTCCCAAGTGCCGATGAAATAATTATAAAG ATGCATGGCAGCCAGCTGACACAAAGATATCTGGAGAAACATGGATTTGATGTCCCTATTATGGTCCCAA AATTAGATGATCTAGGACTCAGGCTCCCTTCACCTACATTTTCTGTGATGGATGTGGAACGTTATGTAGG TGGTGACAAAGTGATAGATGTCATTGATGTGGCGAGGCAGGCAGACAGCAAAATGACACTTCACAATTAT GTTAAATACTTCATGAATCCTAACAGACCAAAAGTGTTAAATGTGATCAGCCTTGAATTTTCAGATACAA AGATGTCTGAATTGGTGGAGGTCCCTGATATAGCCAAAAAACTTTCCTGGGTGGAAAATTATTGGCCAGA TGATTCAGTCTTTCCCAAGCCATTTGTTCAGAAATATTGCTTAATGGGAGTTCAAGACAGCTATACAGAT TTCCACATTGACTTCGGTGGAACTTCAGTCTGGTACCATGTCCTCTGGGGTGAGAAGATTTTTTATTTAA TAAAGCCAACAGATGAAAATTTGGCACGTTATGAATCTTGGAGTTCATCTGTGACCCAGAGTGAGGTGTT CTTTGGAGATAAGGTGGATAAATGCTACAAATGTGTGGTAAAGCAGGGACATACCTTATTTGTTCCTACA GGGTGGATCCATGCTGTGCTCACTTCTCAGGACTGTATGGCTTTTGGGGGGAACTTCCTGCACAACCTTA ACATTGGCATGCAGCTCAGGTGTTATGAGATGGAGAAAAGGCTAAAAACACCAGATCTTTTCAAATTCCC TTTCTTTGAAGCCATATGTTGGTTTGTAGCCAAAAACTTGCTGGAAACCCTGAAAGAACTGAGAGAAGAT GGTTTCCAGCCTCAAACTTACCTAGTACAGGGAGTGAAAGCACTGCATACTGCTTTAAAATTATGGATGA AAAAAGAACTTGTATCTGAACATGCCTTTGAAATTCCAGACAATGTTAGACCTGGACACCTTATTAAAGA ACTTTCTAAAGTAATTCGAGCAATAGAGGAGGAAAACGGCAAACCAGTTAAATCTCAGGGAATTCCTATT GTGTGTCCAGTTTCACGATCCTCAAATGAAGCAACTTCCCCATACCATTCCCGAAGAAAGATGAGGAAAC TTCGAGATCATAATGTCCGAACTCCTTCTAACCTAGACATCCTAGAGCTCCACACAAGGGAGGTCCTCAA AAGATTAGAGATGTGTCCATGGGAAGAGGACATCTTGAGCTCTAAACTGAATGGAAAATTCAACAAACAT CTCCAACCATCCTCCACAGTACCTGAATGGAGAGCGAAAGATAATGATCTACGATTACTGCTGACAAATG GAAGAATAATTAAAGATGAAAGGCAGCCCTTTGCAGATCAAAGTCTTTATACAGCAGATAGTGAAAATGA AGAGGATAAAAGAAGGACAAAAAAGGCAAAAATGAAGATAGAAGAGAGTTCAGGAGTAGAGGGAGTGGAA CATGAAGAATCTCAAAAACCACTGAATGGGTTTTTTACACGTGTGAAATCAGAACTCAGGAGTAGATCAT CAGGATATTCTGATATTTCTGAGTCAGAAGACTCCGGACCCGAGTGCACTGCACTGAAAAGTATCTTTAC CACTGAAGAGTCTGAAAGTTCAGGTGATGAAAAGAAACAAGAAATAACATCCAACTTTAAGGAGGAATCT AATGTGATGAGGAACTTCCTTCAAAAGAGCCAGAAGCCATCTAGAAGTGAAATTCCAATTAAAAGGGAAT GTCCTACCTCGACGAGCACAGAGGAAGAAGCTATTCAGGGCATGCTGTCTATGGCAGGGTTGCACTATTC CACGTGTTTACAAAGGCAAATACAAAGCACAGACTGCAGTGGTGAAAGAAACTCTCTCCAGGATCCCAGC AGCTGCCATGGCAGTAACCATGAGGTTAGGCAGTTGTATCGCTATGATAAACCAGTGGAATGTGGATACC ATGTCAAGACTGAAGATCCAGACTTGAGGACTTCCTCCTGGATTAAACAGTTTGATACTTCCAGATTTCA TCCTCAGGATCTAAGTAGAAGCCAGAAATGCATCAGAAAGGAAGGTTCATCAGAAATTAGTCAGAGGGTA CAAAGTAGGAATTATGTGGACAGCAGCGGCTCAAGCCTTCAGAATGGAAAGTATATGCAGAATTCAAACC TGACTTCGGGGGCGTGCCAGATAAGTAATGGCAGTCTAAGCCCAGAAAGGCCAGTTGGTGAAACTTCCTT CTCGGTGCCCCTTCACCCCACCAAGAGACCGGCATCAAATCCACCACCTATCAGCAACCAGGCAACAAAA GGTAAACGTCCAAAAAAAGGAATGGCAACAGCCAAACAACGTCTTGGGAAGATCCTTAAGTTGAACAGAA ATGGCCATGCACGTTTCTTTGTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >RG218944 representing NM_030647 Red=Cloning site Green=Tags(s) MAGAAAAVAAGAAAGAAAAAVSVAAPGRASAPPPPPPVYCVCRQPYDVNRFMIECDICKDWFHGSCVGVE EHHAVDIDLYHCPNCAVLHGSSLMKKRRNWHRHDYTEIDDGSKPVQAGTRTFIKELRSRVFPSADEIIIK MHGSQLTQRYLEKHGFDVPIMVPKLDDLGLRLPSPTFSVMDVERYVGGDKVIDVIDVARQADSKMTLHNY VKYFMNPNRPKVLNVISLEFSDTKMSELVEVPDIAKKLSWVENYWPDDSVFPKPFVQKYCLMGVQDSYTD FHIDFGGTSVWYHVLWGEKIFYLIKPTDENLARYESWSSSVTQSEVFFGDKVDKCYKCVVKQGHTLFVPT GWIHAVLTSQDCMAFGGNFLHNLNIGMQLRCYEMEKRLKTPDLFKFPFFEAICWFVAKNLLETLKELRED GFQPQTYLVQGVKALHTALKLWMKKELVSEHAFEIPDNVRPGHLIKELSKVIRAIEEENGKPVKSQGIPI VCPVSRSSNEATSPYHSRRKMRKLRDHNVRTPSNLDILELHTREVLKRLEMCPWEEDILSSKLNGKFNKH LQPSSTVPEWRAKDNDLRLLLTNGRIIKDERQPFADQSLYTADSENEEDKRRTKKAKMKIEESSGVEGVE HEESQKPLNGFFTRVKSELRSRSSGYSDISESEDSGPECTALKSIFTTEESESSGDEKKQEITSNFKEES NVMRNFLQKSQKPSRSEIPIKRECPTSTSTEEEAIQGMLSMAGLHYSTCLQRQIQSTDCSGERNSLQDPS SCHGSNHEVRQLYRYDKPVECGYHVKTEDPDLRTSSWIKQFDTSRFHPQDLSRSQKCIRKEGSSEISQRV QSRNYVDSSGSSLQNGKYMQNSNLTSGACQISNGSLSPERPVGETSFSVPLHPTKRPASNPPPISNQATK GKRPKKGMATAKQRLGKILKLNRNGHARFFV TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN	NM_030647
ORF Size	2823 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_030647.2
RefSeq Size	9178 bp
RefSeq ORF	2826 bp
Locus ID	80853
UniProt ID	Q6ZMT4
Gene Summary	Histone demethylase required for brain development. Specifically demethylates dimethylated 'Lys-9' and 'Lys-27' (H3K9me2 and H3K27me2, respectively) of histone H3 and monomethylated histone H4 'Lys-20' residue (H4K20Me1), thereby playing a central role in histone code. Specifically binds trimethylated 'Lys-4' of histone H3 (H3K4me3), affecting histone demethylase specificity: in presence of H3K4me3, it has no demethylase activity toward H3K9me2, while it has high activity toward H3K27me2. Demethylates H3K9me2 in absence of H3K4me3. Has activity toward H4K20Me1 only when nucleosome is used as a substrate and when not histone octamer is used as substrate.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

The use of this cDNA Clones has been cited in the following citations:
Histone demethylase KDM7A reciprocally regulates adipogenic and osteogenic differentiation via regulation of C/EBPα and canonical Wnt signalling ,null, Journal of Cellular and Molecular Medicine ,PubMed ID 30614617 [KDM7A]

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
RC218944	JHDM1D (Myc-DDK-tagged)-Human jumonji C domain containing histone demethylase 1 homolog D (S. cerevisiae) (JHDM1D)	10 ug 产品选项验证失败所有选项都是必需的	CNY 6,896.00 CNY 7,130.00
RC218944L1	Lenti ORF clone of Human jumonji C domain containing histone demethylase 1 homolog D (S. cerevisiae) (JHDM1D), Myc-DDK-tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 9,296.00
RC218944L2	Lenti ORF clone of Human jumonji C domain containing histone demethylase 1 homolog D (S. cerevisiae) (JHDM1D), mGFP tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 9,030.00
RC218944L3	Lenti ORF clone of Human jumonji C domain containing histone demethylase 1 homolog D (S. cerevisiae) (JHDM1D), Myc-DDK-tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 9,030.00
RC218944L4	Lenti ORF clone of Human jumonji C domain containing histone demethylase 1 homolog D (S. cerevisiae) (JHDM1D), mGFP tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 9,030.00
SC310699	JHDM1D (untagged)-Human jumonji C domain containing histone demethylase 1 homolog D (S. cerevisiae) (JHDM1D)	10 ug 产品选项验证失败所有选项都是必需的	CNY 6,896.00

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