UBXN2B (NM_001077619) Human Tagged ORF Clone

CAT#: RG219137

  • TrueORF®

UBXN2B (tGFP-tagged) - Human UBX domain protein 2B (UBXN2B)

ORF Plasmid: DDK tGFP



  "NM_001077619" in other vectors (6)

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CNY 4,370.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


UBXN2B mouse monoclonal antibody, clone OTI3H8 (formerly 3H8)
    • 100 ul

CNY 1,999.00
CNY 2,700.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag TurboGFP
Synonyms p37
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RG219137 representing NM_001077619
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGAGGGCGGAGGCCCTGAGCCCGGCGAGCAGGAGAGGAGGTCTTCCGGGCCGCGGCCTCCGAGCG
CGCGGGATTTGCAGTTGGCCTTGGCAGAATTGTATGAAGATGAGGTGAAGTGCAAATCTTCCAAGTCTAA
TAGACCTAAAGCCACAGTCTTCAAGAGCCCACGGACACCACCTCAACGGTTTTACTCAAGTGAACATGAA
TACAGTGGATTAAATATAGTTCGACCTTCAACTGGGAAAATTGTGAATGAACTTTTCAAAGAGGCAAGGG
AACATGGGGCTGTCCCTCTGAATGAAGCCACAAGAGCTTCAGGTGATGATAAATCTAAGTCATTTACAGG
TGGAGGATACAGATTGGGTAGTTCTTTTTGTAAGCGGTCTGAATATATCTATGGAGAAAATCAGCTGCAA
GATGTTCAGATTTTGCTTAAACTGTGGAGCAATGGTTTCAGTTTAGATGATGGAGAATTGAGACCTTACA
ATGAACCAACAAATGCTCAATTTCTGGAGTCTGTTAAGAGAGGAGAGATTCCCCTGGAGCTTCAGCGCCT
TGTTCATGGTGGCCAAGTGAATTTGGATATGGAGGATCATCAGGATCAAGAATACATAAAACCTAGATTG
AGGTTCAAGGCTTTTAGTGGAGAAGGGCAAAAACTTGGAAGCCTTACACCTGAAATAGTCAGTACACCTT
CCTCTCCAGAAGAGGAGGATAAATCAATACTTAATGCAGTTGTTCTTATTGATGATTCAGTGCCAACAAC
AAAAATTCAAATCAGGTTAGCAGATGGGAGTCGTTTGATACAAAGATTCAATAGTACACACAGGATCCTG
GATGTCCGGAACTTTATTGTACAGTCTCGTCCTGAATTTGCGGCTCTTGACTTTATTCTTGTGACTTCAT
TTCCGAATAAAGAGCTAACAGATGAAAGCCTGACACTGCTAGAAGCAGATATTCTTAACACTGTGTTACT
CCAGCAACTAAAA


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG219137 representing NM_001077619
Red=Cloning site Green=Tags(s)

MAEGGGPEPGEQERRSSGPRPPSARDLQLALAELYEDEVKCKSSKSNRPKATVFKSPRTPPQRFYSSEHE
YSGLNIVRPSTGKIVNELFKEAREHGAVPLNEATRASGDDKSKSFTGGGYRLGSSFCKRSEYIYGENQLQ
DVQILLKLWSNGFSLDDGELRPYNEPTNAQFLESVKRGEIPLELQRLVHGGQVNLDMEDHQDQEYIKPRL
RFKAFSGEGQKLGSLTPEIVSTPSSPEEEDKSILNAVVLIDDSVPTTKIQIRLADGSRLIQRFNSTHRIL
DVRNFIVQSRPEFAALDFILVTSFPNKELTDESLTLLEADILNTVLLQQLK

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001077619
ORF Size 993 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001077619.1, NP_001071087.1
RefSeq Size 5088 bp
RefSeq ORF 996 bp
Locus ID 137886
UniProt ID Q14CS0
Gene Summary Adapter protein required for Golgi and endoplasmic reticulum biogenesis (PubMed:17141156). Involved in Golgi and endoplasmic reticulum maintenance during interphase and in their reassembly at the end of mitosis (PubMed:17141156). The complex formed with VCP has membrane fusion activity; membrane fusion activity requires USO1-GOLGA2 tethering and BET1L (PubMed:17141156). VCPIP1 is also required, but not its deubiquitinating activity (PubMed:17141156). Together with NSFL1C/p47, regulates the centrosomal levels of kinase AURKA/Aurora A during mitotic progression by promoting AURKA removal from centrosomes in prophase (PubMed:23649807). Also, regulates spindle orientation during mitosis (PubMed:23649807).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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