HES7 (NM_032580) Human Tagged ORF Clone
CAT#: RG219701
- TrueORF®
HES7 (tGFP-tagged) - Human hairy and enhancer of split 7 (Drosophila) (HES7), transcript variant 2
ORF Plasmid: DDK
"NM_032580" in other vectors (6)
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CNY 4,000.00
CNY 4,370.00
CNY 1,000.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | bHLHb37; SCDO4 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG219701 representing NM_032580
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTCACCCGGGATCGAGCTGAGAATAGGGACGGCCCCAAGATGCTCAAGCCGCTTGTGGAGAAGCGGC GCCGGGACCGCATCAACCGCAGCCTGGAAGAGCTGAGGCTGCTGCTGCTGGAGCGGACCCGGGACCAGAA CCTCCGGAACCCGAAGCTGGAGAAAGCGGAGATATTGGAGTTCGCCGTGGGCTACTTGAGGGAGCGAAGC CGGGTGGAGCCCCCGGGGGTTCCCCGGTCCCCAGTCCAGGACGCCAAGGCGCTCGCCAGCTGCTACTTGT CCGGTTTCCGCGAGTGCCTGCTTCGCTTGGCGGCCATCGCGCACGACGCCAGCCCGACCGCCCGCGCCCA GCTCTTCTCCGCGCTGCACGGCTATCTGCGCCCCAAACCGCCCCGGCCCAAGCCGGTAGATCCGAGGCCT CCAGCGCCGCGCCCATCCCTGGACCCCGCCGCACCGGCCCTTGGCCCTGCGCTGCACCAGCGCCCCCCAG TGCACCAGGGCCACCCTAGCCCGCGCTGCGCATGGTCCCCATCCCTCTGCTCCCCGCGCGCCGGGGATTC TGGCGCGCCGGCGCCCCTCACCGGACTGCTGCCGCCGCCACCGCCGCCTCACAGACAAGACGGGGCGCCC AAGGCCCCGCTGCCCCCGCCGCCCGCTTTCTGGAGACCTTGGCCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG219701 representing NM_032580
Red=Cloning site Green=Tags(s) MVTRDRAENRDGPKMLKPLVEKRRRDRINRSLEELRLLLLERTRDQNLRNPKLEKAEILEFAVGYLRERS RVEPPGVPRSPVQDAKALASCYLSGFRECLLRLAAIAHDASPTARAQLFSALHGYLRPKPPRPKPVDPRP PAPRPSLDPAAPALGPALHQRPPVHQGHPSPRCAWSPSLCSPRAGDSGAPAPLTGLLPPPPPPHRQDGAP KAPLPPPPAFWRPWP TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_032580 |
ORF Size | 675 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_032580.1, NP_115969.1 |
RefSeq Size | 678 bp |
RefSeq ORF | 678 bp |
Locus ID | 84667 |
UniProt ID | F8VPC9 |
Gene Summary | This gene encodes a member of the hairy and enhancer of split family of bHLH transcription factors. The mouse ortholog of this gene is regulated by Notch signaling. The protein functions as a transcriptional repressor, and is implicated in correct patterning of the axial skeleton. A mutation in this gene has been shown to result in spondylocostal dysostosis. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC219701 | HES7 (Myc-DDK-tagged)-Human hairy and enhancer of split 7 (Drosophila) (HES7), transcript variant 2 |
CNY 2,400.00 |
|
RC219701L1 | Lenti ORF clone of Human hairy and enhancer of split 7 (Drosophila) (HES7), transcript variant 2, Myc-DDK-tagged |
CNY 4,800.00 |
|
RC219701L2 | Lenti ORF clone of Human hairy and enhancer of split 7 (Drosophila) (HES7), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
RC219701L3 | Lenti ORF clone of Human hairy and enhancer of split 7 (Drosophila) (HES7), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC219701L4 | Lenti ORF clone of Human hairy and enhancer of split 7 (Drosophila) (HES7), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
SC305482 | HES7 (untagged)-Human hairy and enhancer of split 7 (Drosophila) (HES7), transcript variant 2 |
CNY 2,400.00 |