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Myelin expression factor 2 (MYEF2) (NM_016132) Human Tagged ORF Clone
CAT＃: RG220717
- TrueORF®
MYEF2 (tGFP-tagged) - Human myelin expression factor 2 (MYEF2)
ORF Plasmid: DDK
"NM_016132" in other vectors (4)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | HsT18564; MEF-2; MST156; MSTP156; myEF-2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG220717 representing NM_016132
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGACGCCAACAAGGCCGAGGTGCCCGGGGCCACTGGTGGCGACAGCCCGCACCTGCAGCCCGCAG AGCCGCCGGGCGAGCCGCGGCGAGAGCCGCACCCCGCGGAGGCGGAGAAGCAGCAGCCGCAGCACAGCAG CAGCTCCAATGGCGTTAAAATGGAGAATGATGAATCAGCAAAAGAAGAGAAATCTGACTTAAAGGAAAAA TCTACAGGAAGTAAGAAGGCCAATAGATTTCATCCTTATTCAAAAGACAAGAATTCGGGCACTGGAGAAA AGAAGGGTCCAAATCGTAACAGAGTTTTCATTAGCAACATCCCATATGACATGAAATGGCAAGCTATTAA AGATCTAATGAGAGAGAAAGTTGGTGAGGTTACATACGTGGAGCTCTTTAAGGATGCGGAAGGAAAATCA AGGGGTTGTGGTGTGGTTGAATTCAAAGATGAAGAATTTGTAAAGAAAGCCCTAGAAACTATGAACAAAT ATGATCTTAGTGGAAGACCCCTTAATATTAAAGAGGATCCTGATGGAGAAAATGCTCGTAGGGCATTGCA GCGAACAGGAGGATCATTTCCAGGAGGACACGTCCCTGATATGGGATCAGGGTTGATGAATTTACCACCT TCCATACTCAATAATCCAAACATTCCTCCTGAAGTCATCAGTAATTTGCAGGCCGGTAGACTTGGTTCCA CAATTTTTGTTGCCAATCTTGACTTCAAAGTTGGTTGGAAGAAGCTAAAGGAAGTGTTCAGCATAGCTGG AACTGTGAAGCGGGCAGATATTAAAGAAGACAAAGATGGCAAGAGCAGAGGAATGGGCACTGTCACTTTT GAGCAAGCAATTGAAGCAGTTCAAGCAATTTCTATGTTCAATGGGCAGTTTTTATTTGATAGACCTATGC ATGTGAAAATGGATGACAAGTCTGTTCCTCATGAAGAGTACCGTTCACATGATGGTAAAACACCACAATT ACCACGTGGTCTTGGAGGCATTGGGATGGGACTTGGTCCGGGTGGACAGCCTATTAGTGCCAGCCAGTTG AACATAGGTGGAGTAATGGGAAATTTAGGTCCAGGTGGTATGGGAATGGATGGTCCAGGTTTTGGAGGAA TGAATAGAATTGGAGGAGGAATAGGGTTTGGTGGTCTGGAAGCAATGAATAGCATGGGAGGATTTGGAGG AGTTGGCCGAATGGGAGAGCTGTACCGTGGTGCGATGACTAGTAGCATGGAGCGAGATTTTGGACGTGGT GATATTGGAATAAATCGAGGCTTTGGAGATTCCTTTGGTAGACTTGGCAGTGCAATGATTGGAGGGTTTG CAGGAAGAATAGGATCTTCTAACATGGGTCCAGTAGGATCTGGAATAAGTGGTGGAATGGGTAGCATGAA CAGTGTGACTGGAGGAATGGGGATGGGACTGGACCGGATGAGTTCCAGCTTTGATAGAATGGGACCAGGT ATAGGAGCTATACTGGAAAGGAGCATCGATATGGATCGAGGATTTTTATCGGGTCCAATGGGAAGCGGAA TGAGAGAGAGAATAGGCTCCAAAGGCAACCAGATATTTGTCAGAAATCTACCTTTTGACTTGACTTGGCA GAAACTAAAAGAGAAATTCAGTCAGTGTGGTCATGTAATGTTTGCAGAAATAAAAATGGAGAATGGAAAG TCAAAAGGCTGTGGAACAGTCAGATTTGACTCCCCAGAATCAGCTGAAAAAGCCTGCAGAATAATGAATG GCATAAAAATCAGTGGCAGAGAAATTGATGTTCGCTTGGATCGTAATGCA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG220717 representing NM_016132
Red=Cloning site Green=Tags(s) MADANKAEVPGATGGDSPHLQPAEPPGEPRREPHPAEAEKQQPQHSSSSNGVKMENDESAKEEKSDLKEK STGSKKANRFHPYSKDKNSGTGEKKGPNRNRVFISNIPYDMKWQAIKDLMREKVGEVTYVELFKDAEGKS RGCGVVEFKDEEFVKKALETMNKYDLSGRPLNIKEDPDGENARRALQRTGGSFPGGHVPDMGSGLMNLPP SILNNPNIPPEVISNLQAGRLGSTIFVANLDFKVGWKKLKEVFSIAGTVKRADIKEDKDGKSRGMGTVTF EQAIEAVQAISMFNGQFLFDRPMHVKMDDKSVPHEEYRSHDGKTPQLPRGLGGIGMGLGPGGQPISASQL NIGGVMGNLGPGGMGMDGPGFGGMNRIGGGIGFGGLEAMNSMGGFGGVGRMGELYRGAMTSSMERDFGRG DIGINRGFGDSFGRLGSAMIGGFAGRIGSSNMGPVGSGISGGMGSMNSVTGGMGMGLDRMSSSFDRMGPG IGAILERSIDMDRGFLSGPMGSGMRERIGSKGNQIFVRNLPFDLTWQKLKEKFSQCGHVMFAEIKMENGK SKGCGTVRFDSPESAEKACRIMNGIKISGREIDVRLDRNA TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_016132 |
| ORF Size | 1800 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_016132.4, NP_057216.2 |
| RefSeq Size | 4544 bp |
| RefSeq ORF | 1803 bp |
| Locus ID | 50804 |
| UniProt ID | Q9P2K5 |
| Domains | RRM |
| Protein Families | Transcription Factors |
| Gene Summary | Transcriptional repressor of the myelin basic protein gene (MBP). Binds to the proximal MB1 element 5'-TTGTCC-3' of the MBP promoter. Its binding to MB1 and function are inhibited by PURA (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC220717 | MYEF2 (Myc-DDK-tagged)-Human myelin expression factor 2 (MYEF2) |
CNY 4,464.00 |
|
| RC220717L3 | Lenti ORF clone of Human myelin expression factor 2 (MYEF2), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC220717L4 | Lenti ORF clone of Human myelin expression factor 2 (MYEF2), mGFP tagged |
CNY 5,890.00 |
|
| SC310227 | MYEF2 (untagged)-Human myelin expression factor 2 (MYEF2) |
CNY 7,220.00 |
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