MYH13 (NM_003802) Human Tagged ORF Clone
CAT#: RG220970
- TrueORF®
MYH13 (tGFP-tagged) - Human myosin, heavy chain 13, skeletal muscle (MYH13)
ORF Plasmid: DDK
"NM_003802" in other vectors (3)
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CNY 15,200.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | MyHC-eo; MyHC-IIL |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG220970 representing NM_003802
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCTCTGACGCAGAAATGGCCATTTTTGGAGAAGCAGCTCCCTACCTCCGGAAACCAGAGAAGGAGA GAATCGAGGCTCAAAATCGTCCATTCGATTCCAAGAAAGCCTGCTTTGTAGCGGATAATAAGGAAATGTA TGTGAAAGGCATGATCCAGACTAGGGAAAATGACAAAGTCATAGTCAAGACCCTCGATGACCGGATGCTC ACTCTGAACAATGACCAGGTCTTCCCCATGAACCCTCCCAAATTTGACAAGATCGAGGACATGGCCATGA TGACTCACCTGCATGAACCTGCTGTTCTGTACAACCTCAAAGAGCGCTATGCAGCCTGGATGATCTACAC CTACTCAGGCCTCTTCTGTGTCACCGTCAACCCCTACAAGTGGCTGCCGGTGTACAAGCCCGAGGTGGTG GCTGCCTACAGAGGCAAAAAGCGCCAGGAGGCCCCGCCCCACATCTTCTCCATCTCTGACAATGCCTATC AGTTCATGCTGACTGATCGAGACAACCAGTCTATCCTCATCACCGGAGAATCCGGGGCTGGGAAGACTGT GAACACCAAGCGTGTCATCCAGTATTTTGCAACAATTGCAGTTACCGGGGACAAGAAGAAGGAGACACAG CCAGGCAAAATGCAGGGAACCCTAGAGGATCAGATCATCCAGGCCAACCCACTGCTGGAGGCCTTTGGAA ATGCCAAGACTGTGAGGAATGACAACTCCTCAAGATTTGGGAAGTTCATTCGGATTCATTTTGGAGCCAC AGGAAAGCTGGCATCGGCAGACATCGAAACTTATCTGTTAGAAAAATCCAGAGTGACGTTTCAATTATCC AGTGAGAGAAGCTATCATATTTTCTACCAAATTATGTCAAACAAGAAGCCAGAACTAATTGACCTGCTTC TGATCTCCACCAACCCCTTCGACTTCCCCTTCGTGAGCCAAGGAGAGGTCACGGTAGCCAGTATCGATGA CAGTGAAGAACTGCTGGCGACAGATAATGCCATTGACATCCTGGGCTTCAGCTCAGAGGAGAAAGTCGGG ATCTACAAACTGACGGGAGCCGTGATGCATTATGGGAACATGAAGTTCAAGCAGAAGCAGCGTGAGGAGC AGGCGGAGCCAGACGGCACCGAAGTGGCTGACAAAGCCGGATACCTGATGGGACTGAATTCTGCAGAAAT GCTGAAGGGCCTGTGCTGTCCAAGGGTGAAGGTTGGCAATGAATATGTCACTAAAGGGCAAAATGTCCAG CAGGTGACCAATTCGGTGGGTGCTCTGGCCAAAGCCGTCTACGAGAAGATGTTCCTGTGGATGGTCACCC GCATCAACCAGCAGTTGGACACCAAGCAGCCCAGGCAGTACTTCATCGGGGTCTTGGACATTGCTGGCTT TGAGATCTTTGATTTCAACAGCCTGGAGCAGCTGTGCATCAACTTCACCAATGAGAAACTGCAACAGTTT TTCAACCACCACATGTTCGTGCTGGAGCAGGAAGAGTACAAGAAGGAAGGCATCGAGTGGGAGTTCATTG ACTTCGGAATGGACCTGGCTGCCTGCATCGAGCTCATCGAGAAGCCTATGGGCATCTTCTCCATCCTGGA AGAGGAGTGCATGTTCCCCAAGGCAACAGACACCTCCTTCAAGAACAAGCTGTATGACCAGCATCTTGGA AAATCCAACAACTTCCAGAAGCCCAAGCCTGCCAAAGGCAAGGCTGAGGCTCACTTCTCGCTGGTGCACT ATGCCGGCACCGTGGACTACAACATCGCCGGCTGGCTGGACAAAAACAAGGACCCCCTGAATGAGACTGT GGTGGGGCTGTACCAGAAGTCTTCGCTGAAGCTTCTCTCCTTCCTTTTTTCCAACTATGCTGGTGCAGAG ACAGGTGACTCCGGAGGAAGCAAGAAGGGCGGGAAGAAGAAGGGCTCCTCTTTCCAGACCGTGTCGGCCG TGTTCAGGGAAAATTTAAACAAATTGATGACTAACTTAAGGAGCACCCACCCTCACTTTGTACGATGTCT GATTCCCAATGAGACCAAGACTCCTGGTGTGATGGACCACTACTTGGTCATGCACCAGCTGCGCTGTAAC GGGGTCCTCGAGGGCATCCGGATTTGCAGGAAGGGATTCCCCAGCCGGATCCTCTATGCTGACTTCAAGC AGCGGTACCGGATCCTCAATGCCAGTGCTATCCCTGAAGGGCAGTTCATTGACAGCAAAAATGCCTCAGA GAAGCTCCTCAACTCCATCGATGTGGACCGGGAGCAGTTCAGGTTCGGCAACACCAAGGTGTTTTTCAAA GCTGGGCTCCTGGGACTTTTGGAGGAGATGAGAGATGAGAAGCTGGTGACGCTGATGACAAGCACGCAGG CGGTGTGCAGGGGGTACCTGATGCGGGTGGAGTTCAAGAAGATGATGGAGAGGAGGGACTCCATCTTCTG CATCCAATACAACATCCGCTCTTTTATGAACGTCAAGCACTGGCCCTGGATGAACCTGTTCTTCAAAATC AAGCCCCTGCTGAAGAGTGCAGAGGCCGAGAAGGAGATGGCCACCATGAAGGAAGACTTTGAGAGGACCA AGGAAGAACTGGCCCGATCTGAGGCTCGCCGGAAGGAGCTGGAGGAGAAAATGGTCTCCCTCCTGCAGGA GAAGAATGACCTCCAATTGCAGGTCCAGTCTGAAACAGAAAATCTGATGGACGCTGAGGAACGGTGTGAA GGACTCATCAAAAGCAAGATCCTACTGGAAGCAAAAGTCAAGGAGCTGACGGAGAGATTGGAAGAGGAAG AGGAGATGAATTCTGAATTGGTTGCCAAGAAGAGGAATCTGGAAGATAAATGCTCCTCTCTCAAGAGAGA CATTGATGATCTGGAGCTGACCTTGACGAAAGTTGAAAAGGAGAAGCATGCCACAGAGAACAAGGTAAAG AATCTTTCCGAAGAAATGACAGCACTTGAAGAAAACATTTCCAAATTGACCAAAGAAAAGAAATCTCTAC AGGAGGCCCATCAGCAAACACTGGATGATCTTCAGGTGGAAGAAGATAAAGTCAATGGTCTAATCAAAAT AAATGCCAAGCTTGAACAGCAAACAGATGATCTTGAGGGTTCCTTAGAGCAGGAGAAGAAACTGCGGGCG GACTTGGAAAGGGCGAAGAGGAAGCTGGAAGGAGATCTGAAAATGTCCCAGGAATCCATTATGGATCTAG AAAATGAAAAGCAGCAAATAGAAGAGAAATTGAAAAAGAAGGAGTTTGAACTCAGTCAGTTACAAGCCAG AATAGATGACGAACAAGTCCACAGTTTGCAGTTTCAAAAGAAGATTAAAGAGCTGCAAGCCCGCATAGAA GAGCTGGAGGAGGAAATTGAAGCGGAACACACGCTCAGAGCCAAGATTGAGAAGCAGCGCTCAGATCTGG CCAGGGAACTGGAGGAGATCAGCGAGAGGCTGGAAGAAGCCAGTGGGGCCACTTCAGCCCAGATTGAGAT GAACAAGAAGAGGGAGGCTGAGTTCCAGAAAATGCGCAGGGACCTGGAGGAGGCCACCCTGCAGCACGAA GCCACAGCAGCCACCCTGAGGAAGAAGCAAGCAGATAGTGTGGCCGAGCTTGGGGAGCAGATTGACAACC TGCAGCGGGTGAAGCAGAAGCTGGAGAAGGAGAAGAGCGAGCTGAAGATGGAGATCGACGACATGGCCAG CAACATCGAGGCTCTCTCCAAGTCAAAGAGTAACATAGAAAGAACGTGCCGGACGGTAGAAGATCAATTT AGTGAAATCAAAGCCAAGGACGAGCAACAGACACAGTTGATCCATGATCTGAACATGCAGAAAGCAAGAC TGCAGACCCAAAATGGGGAGCTGAGCCACCGAGTGGAAGAGAAGGAGTCTCTGATTTCACAGCTGACCAA AAGCAAGCAGGCCCTCACCCAGCAGCTGGAGGAGCTTAAGAGGCAAATGGAAGAAGAAACCAAGGCCAAG AACGCCATGGCGCACGCCCTGCAGTCCTCCCGCCACGACTGTGACCTGCTGCGGGAACAGTATGAGGAGG AGCAGGAAGCCAAGGCCGAGCTGCAGAGGGCGCTGTCCAAGGCCAACAGTGAGGTTGCCCAGTGGAAGAC CAAATACGAGACGGACGCCATTCAGCGCACAGAAGAGCTGGAGGAGGCCAAGAAAAAACTGGCCCAGAGG CTCCAGGAAGCAGAGGAGAAGACGGAGACGGCGAACTCCAAGTGCGCATCGTTGGAGAAAACCAAGCAGA GGCTGCAGGGAGAGGTGGAGGATCTGATGCGGGATCTGGAGCGCTCCCACACCGCCTGTGCCACACTGGA CAAGAAGCAGAGGAACTTCGACAAGGTCCTTGCAGAGTGGAAGCAAAAGCTGGACGAAAGCCAGGCTGAG CTGGAAGCTGCTCAGAAGGAGTCCAGGTCACTCAGCACTGAACTCTTCAAGATGAGGAATGCCTATGAGG AGGTGGTGGACCAGTTAGAGACACTGAGGCGAGAGAACAAAAATCTGCAAGAAGAGATTTCCGACTTAAC TGAGCAGATTGCAGAAACTGGCAAGAATCTTCAGGAAGCGGAAAAGACCAAGAAGCTAGTGGAGCAGGAA AAGTCAGATCTGCAGGTCGCCTTAGAAGAAGTGGAGGGTTCCTTGGAACACGAGGAGAGCAAGATCTTGC GCGTGCAGCTAGAGCTGAGCCAGGTGAAATCCGAGCTAGACCGCAAGGTCATTGAGAAGGATGAAGAAAT CGAGCAGCTAAAAAGAAACAGCCAGCGGGCAGCAGAGGCCCTGCAGAGCGTGCTGGATGCTGAAATCCGC AGCCGGAACGACGCCCTGAGGCTAAAGAAGAAGATGGAGGGAGACCTTAATGAGATGGAGATTCAGCTGG GCCACTCCAACCGCCAGATGGCAGAGACCCAGAGGCATCTGCGCACGGTCCAGGGCCAGCTCAAGGACTC CCAGCTGCATCTCGATGACGCCCTGAGGAGCAATGAGGACCTCAAGGAGCAGCTGGCCATCGTGGAGCGC AGGAATGGCCTCCTGCTGGAGGAGCTGGAGGAAATGAAGGTGGCCCTGGAACAGACGGAGCGGACCCGCA GGCTGTCAGAGCAGGAGCTGCTGGACGCCAGCGACCGCGTGCAGCTCCTGCACTCCCAGAACACAAGCCT GATAAATACCAAGAAAAAACTGGAGGCTGACATAGCTCAGTGCCAGGCAGAGGTGGAGAACTCGATCCAG GAGTCCAGGAACGCAGAGGAGAAGGCCAAGAAGGCCATCACGGATGCTGCCATGATGGCTGAGGAGCTAA AGAAGGAACAGGACACCAGCGCCCACCTGGAGCGGATGAAGAAGAACCTGGAGCAGACGGTGAAGGACCT GCAGCACCGTCTAGATGAGGCTGAACAACTGGCGCTGAAGGGCGGGAAGAAGCAGATCCAGAAACTGGAG AACCGGGTGCGGGAGCTGGAAAATGAGCTTGATGTGGAACAGAAGAGGGGAGCTGAAGCCCTGAAGGGAG CCCACAAGTACGAACGCAAAGTCAAGGAGATGACTTACCAGGCTGAGGAGGACCGCAAGAATATCCTTAG GCTCCAGGACCTGGTGGACAAGCTGCAGGCCAAAGTGAAGTCTTACAAGAGGCAGGCTGAGGAGGCGGAG GAGCAGGCCAACACGCAGCTGTCCAGATGCCGGAGAGTCCAGCATGAGCTAGAGGAGGCCGCGGAGAGGG CGGACATCGCTGAGTCCCAGGTCAACAAGCTGAGGGCCAAGAGCCGAGACGTGGGCAGCCAGAAGATGGA AGAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG220970 representing NM_003802
Red=Cloning site Green=Tags(s) MSSDAEMAIFGEAAPYLRKPEKERIEAQNRPFDSKKACFVADNKEMYVKGMIQTRENDKVIVKTLDDRML TLNNDQVFPMNPPKFDKIEDMAMMTHLHEPAVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYKPEVV AAYRGKKRQEAPPHIFSISDNAYQFMLTDRDNQSILITGESGAGKTVNTKRVIQYFATIAVTGDKKKETQ PGKMQGTLEDQIIQANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVTFQLS SERSYHIFYQIMSNKKPELIDLLLISTNPFDFPFVSQGEVTVASIDDSEELLATDNAIDILGFSSEEKVG IYKLTGAVMHYGNMKFKQKQREEQAEPDGTEVADKAGYLMGLNSAEMLKGLCCPRVKVGNEYVTKGQNVQ QVTNSVGALAKAVYEKMFLWMVTRINQQLDTKQPRQYFIGVLDIAGFEIFDFNSLEQLCINFTNEKLQQF FNHHMFVLEQEEYKKEGIEWEFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDTSFKNKLYDQHLG KSNNFQKPKPAKGKAEAHFSLVHYAGTVDYNIAGWLDKNKDPLNETVVGLYQKSSLKLLSFLFSNYAGAE TGDSGGSKKGGKKKGSSFQTVSAVFRENLNKLMTNLRSTHPHFVRCLIPNETKTPGVMDHYLVMHQLRCN GVLEGIRICRKGFPSRILYADFKQRYRILNASAIPEGQFIDSKNASEKLLNSIDVDREQFRFGNTKVFFK AGLLGLLEEMRDEKLVTLMTSTQAVCRGYLMRVEFKKMMERRDSIFCIQYNIRSFMNVKHWPWMNLFFKI KPLLKSAEAEKEMATMKEDFERTKEELARSEARRKELEEKMVSLLQEKNDLQLQVQSETENLMDAEERCE GLIKSKILLEAKVKELTERLEEEEEMNSELVAKKRNLEDKCSSLKRDIDDLELTLTKVEKEKHATENKVK NLSEEMTALEENISKLTKEKKSLQEAHQQTLDDLQVEEDKVNGLIKINAKLEQQTDDLEGSLEQEKKLRA DLERAKRKLEGDLKMSQESIMDLENEKQQIEEKLKKKEFELSQLQARIDDEQVHSLQFQKKIKELQARIE ELEEEIEAEHTLRAKIEKQRSDLARELEEISERLEEASGATSAQIEMNKKREAEFQKMRRDLEEATLQHE ATAATLRKKQADSVAELGEQIDNLQRVKQKLEKEKSELKMEIDDMASNIEALSKSKSNIERTCRTVEDQF SEIKAKDEQQTQLIHDLNMQKARLQTQNGELSHRVEEKESLISQLTKSKQALTQQLEELKRQMEEETKAK NAMAHALQSSRHDCDLLREQYEEEQEAKAELQRALSKANSEVAQWKTKYETDAIQRTEELEEAKKKLAQR LQEAEEKTETANSKCASLEKTKQRLQGEVEDLMRDLERSHTACATLDKKQRNFDKVLAEWKQKLDESQAE LEAAQKESRSLSTELFKMRNAYEEVVDQLETLRRENKNLQEEISDLTEQIAETGKNLQEAEKTKKLVEQE KSDLQVALEEVEGSLEHEESKILRVQLELSQVKSELDRKVIEKDEEIEQLKRNSQRAAEALQSVLDAEIR SRNDALRLKKKMEGDLNEMEIQLGHSNRQMAETQRHLRTVQGQLKDSQLHLDDALRSNEDLKEQLAIVER RNGLLLEELEEMKVALEQTERTRRLSEQELLDASDRVQLLHSQNTSLINTKKKLEADIAQCQAEVENSIQ ESRNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNLEQTVKDLQHRLDEAEQLALKGGKKQIQKLE NRVRELENELDVEQKRGAEALKGAHKYERKVKEMTYQAEEDRKNILRLQDLVDKLQAKVKSYKRQAEEAE EQANTQLSRCRRVQHELEEAAERADIAESQVNKLRAKSRDVGSQKMEE TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_003802 |
ORF Size | 5814 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_003802.1, NP_003793.1 |
RefSeq Size | 5992 bp |
RefSeq ORF | 5817 bp |
Locus ID | 8735 |
UniProt ID | Q9UKX3 |
Protein Pathways | Tight junction, Viral myocarditis |
Gene Summary | Fast twitching myosin mediating the high-velocity and low-tension contractions of specific striated muscles.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC220970 | MYH13 (Myc-DDK-tagged)-Human myosin, heavy chain 13, skeletal muscle (MYH13) |
CNY 13,024.00 |
|
RC220970L1 | Lenti ORF clone of Human myosin, heavy chain 13, skeletal muscle (MYH13), Myc-DDK-tagged |
CNY 15,424.00 |
|
SC303379 | MYH13 (untagged)-Human myosin, heavy chain 13, skeletal muscle (MYH13) |
CNY 37,240.00 |