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PKN2 (NM_006256) Human Tagged ORF Clone
CAT＃: RG220989
- TrueORF®
PKN2 (tGFP-tagged) - Human protein kinase N2 (PKN2)
ORF Plasmid: DDK
"NM_006256" in other vectors (7)
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CNY 8,808.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Pak-2; PAK2; PRK2; PRKCL2; PRO2042; STK7 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG220989 representing NM_006256
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGTCCAACCCCGAACGGGGGGAGATTCTGCTCACGGAACTGCAGGGGGATTCCCGAAGTCTTCCGT TTTCTGAGAATGTGAGTGCTGTTCAAAAATTAGACTTTTCAGATACAATGGTGCAGCAGAAATTGGATGA TATCAAGGATCGAATTAAGAGAGAAATAAGGAAAGAACTGAAAATCAAAGAAGGAGCTGAAAATCTGAGG AAAGTCACAACAGATAAAAAAAGTTTGGCTTATGTAGACAACATTTTGAAAAAATCAAATAAAAAATTAG AAGAACTACATCACAAGCTGCAGGAATTAAATGCACATATTGTTGTATCAGATCCAGAAGATATTACAGA TTGCCCAAGGACTCCAGATACTCCAAATAATGACCCTCGTTGTTCTACTAGCAACAATAGATTGAAGGCC TTACAAAAACAATTGGATATAGAACTTAAAGTAAAACAAGGTGCAGAGAATATGATACAGATGTATTCAA ATGGATCTTCAAAGGATCGGAAACTCCATGGTACAGCTCAGCAACTGCTCCAGGACAGCAAGACAAAAAT AGAAGTCATACGAATGCAGATTCTTCAGGCAGTCCAGACTAATGAATTGGCTTTTGATAATGCAAAACCT GTGATAAGTCCTCTTGAACTTCGGATGGAAGAATTAAGGCATCATTTTAGGATAGAGTTTGCAGTAGCAG AAGGTGCAAAGAATGTAATGAAATTACTTGGCTCAGGAAAAGTAACAGACAGAAAAGCACTTTCAGAAGC TCAAGCAAGATTTAATGAATCAAGTCAGAAGTTGGACCTTTTAAAGTATTCATTAGAGCAAAGATTAAAC GAAGTCCCCAAGAATCATCCCAAAAGCAGGATTATTATTGAAGAACTTTCACTTGTTGCTGCATCACCAA CACTAAGTCCACGTCAAAGTATGATATCTACGCAAAATCAATATAGTACACTATCCAAACCAGCAGCACT AACAGGTACTTTGGAAGTTCGTCTTATGGGCTGCCAAGATATCCTAGAGAATGTCCCTGGACGGTCAAAA GCAACATCAGTTGCACTGCCTGGTTGGAGTCCAAGTGAAACCAGATCATCTTTCATGAGCAGAACGAGTA AAAGTAAAAGCGGAAGTAGTCGAAATCTTCTAAAAACCGATGACTTGTCCAATGATGTCTGTGCTGTTTT GAAGCTCGATAATACTGTGGTTGGCCAAACTAGCTGGAAACCCATTTCCAATCAGTCATGGGACCAGAAG TTTACACTGGAACTGGACAGGTCACGTGAACTGGAAATTTCAGTTTATTGGCGTGATTGGCGGTCTCTGT GTGCTGTAAAATTTCTGAGGTTAGAAGATTTTTTAGACAACCAACGGCATGGCATGTGTCTCTATTTGGA ACCACAGGGTACTTTATTTGCAGAGGTTACCTTTTTTAATCCAGTTATTGAAAGAAGACCAAAACTTCAA AGACAAAAGAAAATTTTTTCAAAGCAACAAGGCAAAACATTTCTCAGAGCTCCTCAAATGAATATTAATA TTGCCACTTGGGGAAGGCTAGTAAGAAGAGCTATTCCTACAGTAAATCATTCTGGCACCTTCAGCCCTCA AGCTCCTGTGCCTACTACAGTGCCAGTGGTTGATGTACGCATCCCTCAACTAGCACCTCCAGCTAGTGAT TCTACAGTAACCAAATTGGACTTTGATCTTGAGCCTGAACCTCCTCCAGCCCCACCACGAGCTTCTTCTC TTGGAGAAATAGATGAATCTTCTGAATTAAGAGTTTTGGATATACCAGGACAGGATTCAGAGACTGTTTT TGATATTCAGAATGACAGAAATAGTATACTTCCAAAATCTCAATCTGAATACAAGCCTGATACTCCTCAG TCAGGCCTAGAATATAGTGGTATTCAAGAACTTGAGGACAGAAGATCTCAGCAAAGGTTTCAGTTTAATC TACAAGATTTCAGGTGTTGTGCTGTCTTGGGAAGAGGACATTTTGGAAAGGTGCTTTTAGCTGAATATAA AAACACAAATGAGATGTTTGCTATAAAAGCCTTAAAGAAAGGAGATATTGTGGCTCGAGATGAAGTAGAC AGCCTGATGTGTGAAAAAAGAATTTTTGAAACTGTGAATAGTGTAAGGCATCCCTTTTTGGTGAACCTTT TTGCATGTTTCCAAACCAAAGAGCATGTTTGCTTTGTAATGGAATATGCTGCCGGTGGGGACCTAATGAT GCACATTCATACTGATGTCTTTTCTGAACCAAGAGCTGTATTTTATGCTGCTTGTGTAGTTCTTGGGTTG CAGTATTTACATGAACACAAAATTGTTTATAGAGATTTGAAATTGGATAACTTATTGCTAGATACAGAGG GCTTTGTGAAAATTGCTGATTTTGGTCTTTGCAAAGAAGGAATGGGATATGGAGATAGAACAAGCACATT TTGTGGCACTCCTGAATTTCTTGCCCCAGAAGTATTAACAGAAACTTCTTATACAAGGGCTGTAGATTGG TGGGGCCTTGGCGTGCTTATATATGAAATGCTTGTTGGTGAGTCTCCCTTTCCTGGTGATGATGAAGAGG AAGTTTTTGACAGTATTGTAAATGATGAAGTAAGGTATCCAAGGTTCTTATCTACAGAAGCCATTTCTAT AATGAGAAGGCTGTTAAGAAGAAATCCTGAACGGCGCCTTGGGGCTAGCGAGAAAGATGCAGAGGATGTA AAAAAGCACCCATTTTTCCGGCTAATTGATTGGAGCGCTCTGATGGACAAAAAAGTAAAGCCACCATTTA TACCTACCATAAGAGGACGAGAAGATGTTAGTAATTTTGATGATGAATTTACCTCAGAAGCACCTATTCT GACTCCACCTCGAGAACCAAGGATACTTTCGGAAGAGGAGCAGGAAATGTTCAGAGATTTTGACTACATT GCTGATTGGTGT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG220989 representing NM_006256
Red=Cloning site Green=Tags(s) MASNPERGEILLTELQGDSRSLPFSENVSAVQKLDFSDTMVQQKLDDIKDRIKREIRKELKIKEGAENLR KVTTDKKSLAYVDNILKKSNKKLEELHHKLQELNAHIVVSDPEDITDCPRTPDTPNNDPRCSTSNNRLKA LQKQLDIELKVKQGAENMIQMYSNGSSKDRKLHGTAQQLLQDSKTKIEVIRMQILQAVQTNELAFDNAKP VISPLELRMEELRHHFRIEFAVAEGAKNVMKLLGSGKVTDRKALSEAQARFNESSQKLDLLKYSLEQRLN EVPKNHPKSRIIIEELSLVAASPTLSPRQSMISTQNQYSTLSKPAALTGTLEVRLMGCQDILENVPGRSK ATSVALPGWSPSETRSSFMSRTSKSKSGSSRNLLKTDDLSNDVCAVLKLDNTVVGQTSWKPISNQSWDQK FTLELDRSRELEISVYWRDWRSLCAVKFLRLEDFLDNQRHGMCLYLEPQGTLFAEVTFFNPVIERRPKLQ RQKKIFSKQQGKTFLRAPQMNINIATWGRLVRRAIPTVNHSGTFSPQAPVPTTVPVVDVRIPQLAPPASD STVTKLDFDLEPEPPPAPPRASSLGEIDESSELRVLDIPGQDSETVFDIQNDRNSILPKSQSEYKPDTPQ SGLEYSGIQELEDRRSQQRFQFNLQDFRCCAVLGRGHFGKVLLAEYKNTNEMFAIKALKKGDIVARDEVD SLMCEKRIFETVNSVRHPFLVNLFACFQTKEHVCFVMEYAAGGDLMMHIHTDVFSEPRAVFYAACVVLGL QYLHEHKIVYRDLKLDNLLLDTEGFVKIADFGLCKEGMGYGDRTSTFCGTPEFLAPEVLTETSYTRAVDW WGLGVLIYEMLVGESPFPGDDEEEVFDSIVNDEVRYPRFLSTEAISIMRRLLRRNPERRLGASEKDAEDV KKHPFFRLIDWSALMDKKVKPPFIPTIRGREDVSNFDDEFTSEAPILTPPREPRILSEEEQEMFRDFDYI ADWC TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_006256 |
| ORF Size | 2952 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_006256.4 |
| RefSeq Size | 3255 bp |
| RefSeq ORF | 2955 bp |
| Locus ID | 5586 |
| UniProt ID | Q16513 |
| Domains | C2, pkinase, HR1, S_TK_X, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | PKC-related serine/threonine-protein kinase and Rho/Rac effector protein that participates in specific signal transduction responses in the cell. Plays a role in the regulation of cell cycle progression, actin cytoskeleton assembly, cell migration, cell adhesion, tumor cell invasion and transcription activation signaling processes. Phosphorylates CTTN in hyaluronan-induced astrocytes and hence decreases CTTN ability to associate with filamentous actin. Phosphorylates HDAC5, therefore lead to impair HDAC5 import. Direct RhoA target required for the regulation of the maturation of primordial junctions into apical junction formation in bronchial epithelial cells. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Stimulates FYN kinase activity that is required for establishment of skin cell-cell adhesion during keratinocytes differentiation. Regulates epithelial bladder cells speed and direction of movement during cell migration and tumor cell invasion. Inhibits Akt pro-survival-induced kinase activity. Mediates Rho protein-induced transcriptional activation via the c-fos serum response factor (SRF). Involved in the negative regulation of ciliogenesis (PubMed:27104747).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC220989 | PKN2 (Myc-DDK-tagged)-Human protein kinase N2 (PKN2) |
CNY 7,208.00 |
|
| RC220989L1 | Lenti ORF clone of Human protein kinase N2 (PKN2), Myc-DDK-tagged |
CNY 9,608.00 |
|
| RC220989L2 | Lenti ORF clone of Human protein kinase N2 (PKN2), mGFP tagged |
CNY 11,690.00 |
|
| RC220989L3 | Lenti ORF clone of Human protein kinase N2 (PKN2), Myc-DDK-tagged |
CNY 11,690.00 |
|
| RC220989L4 | Lenti ORF clone of Human protein kinase N2 (PKN2), mGFP tagged |
CNY 11,690.00 |
|
| SC108568 | PKN2 (untagged)-Human protein kinase N2 (PKN2) |
CNY 7,216.00 |
|
| SC323396 | PKN2 (untagged)-Kinase deficient mutant (K686M) of Human protein kinase N2 (PKN2) |
CNY 7,216.00 |
