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WDR74 (NM_018093) Human Tagged ORF Clone
CAT＃: RG221134
- TrueORF®
WDR74 (tGFP-tagged) - Human WD repeat domain 74 (WDR74)
ORF Plasmid: DDK
"NM_018093" in other vectors (4)
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CNY 5,256.00
Product images
CNY 1,000.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Nsa1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG221134 representing NM_018093
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCTGCTGCTGCACGCTGGAACCATGTGTGGGTCGGCACCGAGACTGGGATCTTGAAAGGGGTAA ATCTTCAGCGAAAACAGGCGGCGAACCTCACGGCCGGAGGACAGCCGCGGCGCGAGGAGGCAGTGAGCGC CCTGTGTTGGGGCACCGGCGGCGAGACCCAGATGCTGGTGGGCTGCGCGGACAGGACGGTGAAGCACTTC AGCACCGAGGATGGCATATTCCAGGGTCAGAGACACTGCCCGGGCGGGGAGGGCATGTTCCGTGGCCTCG CCCAGGCCGACGGCACCCTCATCACATGTGTGGATTCTGGGATTCTCAGAGTCTGGCATGACAAGGACAA GGACACATCCTCTGACCCACTCCTGGAACTGAGAGTGGGCCCTGGGGTGTGTAGGATGCGCCAAGACCCA GCACACCCCCATGTGGTTGCCACAGGTGGGAAAGAGAATGCTTTGAAGATATGGGACCTGCAGGGCTCTG AGGAACCTGTGTTCAGGGCCAAGAACGTGCGGAATGACTGGCTGGACTTGCGGGTTCCCATCTGGGACCA GGACATACAGTTTCTCCCAGGATCACAGAAGCTTGTCACCTGCACAGGGTACCACCAGGTCCGTGTTTAT GATCCAGCATCCCCCCAGCGCCGGCCAGTCCTAGAGACCACCTATGGAGAGTACTCACTAACAGCCATGA CCCTCACTCCGGGAGGCAACTCAGTGATTGTGGGAAACACTCATGGGCAGCTGGCAGAAATTGACCTTCG GCAAGGGCGTCTACTGGGCTGTCTGAAGGGGCTGGCAGGCAGTGTGCGTGGGTTGCAGTGCCACCCTTCA AAGCCTCTACTAGCCTCCTGTGGCTTGGACAGAGTCTTGAGGATACACGGGACCCAGAATCCACGGGGTC TGGAGCATAAGGTTTATCTCAAGTCTCAATTGAACTGCCTCCTCTTGTCAGGCAGGGACAACTGGGAGGA TGAGCCCCAAGAGCCTCAAGAACCCAACAAGGTGCCCCTAGAAGACACAGAGACAGATGAACTTTGGGCA TCCTTGGAGGCAGCTGCCAAGCGGAAGCTCTCGGGTTTGGAGCAGCCCCAAGGAGCTCTCCAAACGAGAC GGAGAAAGAAGAAGCGGCCTGGGTCCACCAGCCCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG221134 representing NM_018093
Red=Cloning site Green=Tags(s) MAAAAARWNHVWVGTETGILKGVNLQRKQAANLTAGGQPRREEAVSALCWGTGGETQMLVGCADRTVKHF STEDGIFQGQRHCPGGEGMFRGLAQADGTLITCVDSGILRVWHDKDKDTSSDPLLELRVGPGVCRMRQDP AHPHVVATGGKENALKIWDLQGSEEPVFRAKNVRNDWLDLRVPIWDQDIQFLPGSQKLVTCTGYHQVRVY DPASPQRRPVLETTYGEYSLTAMTLTPGGNSVIVGNTHGQLAEIDLRQGRLLGCLKGLAGSVRGLQCHPS KPLLASCGLDRVLRIHGTQNPRGLEHKVYLKSQLNCLLLSGRDNWEDEPQEPQEPNKVPLEDTETDELWA SLEAAAKRKLSGLEQPQGALQTRRRKKKRPGSTSP TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_018093 |
| ORF Size | 1155 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_018093.1, NP_060563.1 |
| RefSeq Size | 1366 bp |
| RefSeq ORF | 1158 bp |
| Locus ID | 54663 |
| UniProt ID | Q6RFH5 |
| Domains | WD40 |
| Gene Summary | Regulatory protein of the MTREX-exosome complex involved in the synthesis of the 60S ribosomal subunit (PubMed:26456651). Participates in an early cleavage of the pre-rRNA processing pathway in cooperation with NVL (PubMed:29107693). Required for blastocyst formation, is necessary for RNA transcription, processing and/or stability during preimplantation development (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221134 | WDR74 (Myc-DDK-tagged)-Human WD repeat domain 74 (WDR74) |
CNY 3,656.00 |
|
| RC221134L3 | Lenti ORF clone of Human WD repeat domain 74 (WDR74), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC221134L4 | Lenti ORF clone of Human WD repeat domain 74 (WDR74), mGFP tagged |
CNY 5,890.00 |
|
| SC113772 | WDR74 (untagged)-Human WD repeat domain 74 (WDR74) |
CNY 6,270.00 |
