CLOCK (NM_004898) Human Tagged ORF Clone
CAT#: RG221408
- TrueORF®
CLOCK (tGFP-tagged) - Human clock homolog (mouse) (CLOCK)
ORF Plasmid: DDK
"NM_004898" in other vectors (6)
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CNY 4,370.00
Cited in 1 publication. |
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | bHLHe8; KAT13D |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG221408 representing NM_004898
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTGTTTACCGTAAGCTGTAGTAAAATGAGCTCGATTGTTGACAGAGATGACAGTAGTATTTTTGATG GGTTGGTGGAAGAAGATGACAAGGACAAAGCGAAAAGAGTATCTAGAAACAAATCTGAAAAGAAACGTAG AGATCAATTTAATGTTCTCATTAAAGAACTGGGATCCATGCTTCCTGGTAATGCTAGAAAGATGGACAAA TCTACTGTTCTGCAGAAAAGCATTGATTTTTTACGAAAACATAAAGAAATCACTGCACAGTCAGATGCTA GTGAAATTCGACAGGACTGGAAACCTACATTCCTTAGTAATGAAGAGTTTACACAATTAATGTTAGAGGC TCTTGATGGTTTTTTTTTAGCAATCATGACAGATGGAAGCATAATATATGTGTCTGAGAGTGTAACTTCA TTACTTGAACATTTACCATCTGATCTTGTGGATCAAAGTATATTTAATTTTATCCCAGAAGGGGAACATT CAGAGGTTTATAAAATACTCTCTACTCATCTGCTGGAAAGTGATTCATTAACCCCAGAATATTTAAAATC AAAAAATCAGTTAGAATTCTGTTGTCACATGCTGCGAGGAACAATAGACCCAAAGGAGCCATCTACCTAT GAATATGTAAAATTTATAGGAAATTTCAAATCTTTAAACAGTGTATCCTCTTCAGCACACAATGGTTTTG AAGGAACTATACAACGCACACATAGGCCATCTTATGAAGATAGAGTTTGTTTTGTAGCTACTGTCAGGTT AGCTACACCTCAGTTCATCAAGGAAATGTGCACTGTTGAAGAACCCAATGAAGAGTTTACATCTAGACAT AGTTTAGAATGGAAGTTTCTGTTTCTAGATCACAGGGCACCACCCATAATAGGGTATTTGCCATTTGAAG TTCTGGGAACATCAGGCTATGATTACTATCATGTGGATGACCTAGAAAATTTGGCAAAATGTCATGAGCA CTTAATGCAATATGGGAAAGGCAAATCATGTTATTATAGGTTCCTGACTAAGGGGCAACAGTGGATTTGG CTTCAGACTCATTATTATATCACTTACCATCAGTGGAATTCAAGGCCAGAGTTTATTGTTTGTACTCACA CTGTAGTAAGTTATGCAGAAGTTAGGGCTGAAAGACGACGAGAACTTGGCATTGAAGAGTCTCTTCCTGA GACAGCTGCTGACAAAAGCCAAGATTCTGGGTCAGATAATCGTATAAACACAGTCAGTCTCAAGGAAGCA TTGGAAAGGTTTGATCACAGCCCAACCCCTTCTGCCTCTTCTCGGAGTTCAAGAAAATCATCTCACACGG CCGTCTCAGACCCTTCCTCAACACCAACCAAGATCCCGACGGATACGAGCACTCCACCCAGGCAGCATTT ACCAGCTCATGAGAAGATGGTGCAAAGAAGGTCATCATTTAGTAGTCAGTCCATAAATTCCCAGTCTGTT GGTTCATCATTAACACAGCCAGTGATGTCTCAAGCTACAAATTTACCAATTCCACAAGGCATGTCCCAGT TTCAGTTTTCAGCTCAATTAGGAGCCATGCAACATCTGAAAGACCAATTGGAACAACGGACACGCATGAT AGAAGCAAATATTCATCGGCAACAAGAAGAACTAAGAAAAATTCAAGAACAACTTCAGATGGTCCATGGT CAGGGGCTGCAGATGTTTTTGCAACAATCAAATCCTGGGTTGAATTTTGGTTCCGTTCAACTTTCTTCTG GAAATTCATCTAACATCCAGCAACTTGCACCTATAAATATGCAAGGCCAAGTTGTTCCTACTAACCAGAT TCAAAGTGGAATGAATACTGGACACATTGGCACAACTCAGCACATGATACAACAACAGACTTTACAGAGT ACATCAACTCAGAGTCAACAAAATGTACTGAGTGGGCACAGTCAGCAAACATCTCTACCCAGTCAGACAC AGAGCACTCTTACAGCCCCACTGTATAACACTATGGTGATTTCTCAGCCTGCAGCCGGAAGCATGGTCCA GATTCCATCTAGTATGCCACAAAACAGCACCCAGAGTGCTGCAGTAACTACATTCACTCAGGACAGGCAG ATAAGATTTTCTCAAGGTCAACAACTTGTGACCAAATTAGTGACTGCTCCTGTAGCTTGTGGGGCAGTCA TGGTACCTAGTACTATGCTTATGGGCCAGGTGGTGACTGCATATCCTACTTTTGCTACACAACAGCAACA GTCACAGACATTGTCAGTAACGCAGCAGCAGCAGCAGCAGAGCTCCCAGGAGCAGCAGCTCACTTCAGTT CAGCAACCATCTCAGGCTCAGCTGACCCAGCCACCGCAACAATTTTTACAGACTTCTAGGTTGCTCCATG GGAATCCCTCAACTCAACTCATTCTCTCTGCTGCATTTCCTCTACAACAGAGCACCTTCCCTCAGTCACA TCACCAGCAACATCAGTCTCAGCAACAGCAGCAACTCAGCCGGCACAGGACTGACAGCTTGCCCGACCCT TCCAAGGTTCAACCACAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG221408 representing NM_004898
Red=Cloning site Green=Tags(s) MLFTVSCSKMSSIVDRDDSSIFDGLVEEDDKDKAKRVSRNKSEKKRRDQFNVLIKELGSMLPGNARKMDK STVLQKSIDFLRKHKEITAQSDASEIRQDWKPTFLSNEEFTQLMLEALDGFFLAIMTDGSIIYVSESVTS LLEHLPSDLVDQSIFNFIPEGEHSEVYKILSTHLLESDSLTPEYLKSKNQLEFCCHMLRGTIDPKEPSTY EYVKFIGNFKSLNSVSSSAHNGFEGTIQRTHRPSYEDRVCFVATVRLATPQFIKEMCTVEEPNEEFTSRH SLEWKFLFLDHRAPPIIGYLPFEVLGTSGYDYYHVDDLENLAKCHEHLMQYGKGKSCYYRFLTKGQQWIW LQTHYYITYHQWNSRPEFIVCTHTVVSYAEVRAERRRELGIEESLPETAADKSQDSGSDNRINTVSLKEA LERFDHSPTPSASSRSSRKSSHTAVSDPSSTPTKIPTDTSTPPRQHLPAHEKMVQRRSSFSSQSINSQSV GSSLTQPVMSQATNLPIPQGMSQFQFSAQLGAMQHLKDQLEQRTRMIEANIHRQQEELRKIQEQLQMVHG QGLQMFLQQSNPGLNFGSVQLSSGNSSNIQQLAPINMQGQVVPTNQIQSGMNTGHIGTTQHMIQQQTLQS TSTQSQQNVLSGHSQQTSLPSQTQSTLTAPLYNTMVISQPAAGSMVQIPSSMPQNSTQSAAVTTFTQDRQ IRFSQGQQLVTKLVTAPVACGAVMVPSTMLMGQVVTAYPTFATQQQQSQTLSVTQQQQQQSSQEQQLTSV QQPSQAQLTQPPQQFLQTSRLLHGNPSTQLILSAAFPLQQSTFPQSHHQQHQSQQQQQLSRHRTDSLPDP SKVQPQ TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_004898 |
ORF Size | 2538 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_004898.2, NP_004889.1 |
RefSeq Size | 5801 bp |
RefSeq ORF | 2541 bp |
Locus ID | 9575 |
UniProt ID | O15516 |
Domains | PAS, HLH, PAC |
Protein Families | Druggable Genome, Transcription Factors |
Protein Pathways | Circadian rhythm - mammal |
Gene Summary | The protein encoded by this gene plays a central role in the regulation of circadian rhythms. The protein encodes a transcription factor of the basic helix-loop-helix (bHLH) family and contains DNA binding histone acetyltransferase activity. The encoded protein forms a heterodimer with ARNTL (BMAL1) that binds E-box enhancer elements upstream of Period (PER1, PER2, PER3) and Cryptochrome (CRY1, CRY2) genes and activates transcription of these genes. PER and CRY proteins heterodimerize and repress their own transcription by interacting in a feedback loop with CLOCK/ARNTL complexes. Polymorphisms in this gene may be associated with behavioral changes in certain populations and with obesity and metabolic syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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Elevated CLOCK and BMAL1 Contribute to the Impairment of Aerobic Glycolysis from Astrocytes in Alzheimer’s Disease
,null,
International Journal of Molecular Sciences
,PubMed ID 33114015
[CLOCK]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC221408 | CLOCK (Myc-DDK-tagged)-Human clock homolog (mouse) (CLOCK) |
CNY 6,200.00 |
|
RC221408L1 | Lenti ORF clone of Human clock homolog (mouse) (CLOCK), Myc-DDK-tagged |
CNY 8,600.00 |
|
RC221408L2 | Lenti ORF clone of Human clock homolog (mouse) (CLOCK), mGFP tagged |
CNY 5,890.00 |
|
RC221408L3 | Lenti ORF clone of Human clock homolog (mouse) (CLOCK), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC221408L4 | Lenti ORF clone of Human clock homolog (mouse) (CLOCK), mGFP tagged |
CNY 8,600.00 |
|
SC117088 | CLOCK (untagged)-Human clock homolog (mouse) (CLOCK) |
CNY 6,208.00 |