FACL4 (ACSL4) (NM_022977) Human Tagged ORF Clone
CAT#: RG221413
- TrueORF®
ACSL4 (tGFP-tagged) - Human acyl-CoA synthetase long-chain family member 4 (ACSL4), transcript variant 2
ORF Plasmid: DDK
"NM_022977" in other vectors (4)
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CNY 6,808.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | ACS4; FACL4; LACS4; MRX63; MRX68 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG221413 representing NM_022977
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAACTTAAGCTAAATGTGCTCACCATTATTTTGCTGCCTGTCCACTTGTTAATAACAATATACAGTG CCCTTATATTTATTCCATGGTATTTTCTTACCAATGCCAAGAAGAAAAACGCTATGGCAAAGAGAATAAA AGCTAAGCCCACTTCAGACAAACCTGGAAGTCCATATCGCTCTGTCACACACTTCGACTCACTAGCTGTA ATAGACATCCCTGGAGCAGATACTCTGGATAAATTATTTGACCATGCTGTATCCAAGTTTGGGAAGAAGG ACAGCCTTGGGACCAGGGAAATCCTAAGTGAAGAAAATGAAATGCAGCCAAATGGAAAAGTTTTTAAGAA GTTAATTCTTGGGAATTATAAATGGATGAACTATCTTGAAGTGAATCGCAGAGTGAATAACTTTGGTAGT GGACTCACTGCACTGGGACTAAAACCAAAGAACACCATTGCCATCTTCTGTGAGACCAGGGCCGAATGGA TGATTGCAGCACAGACCTGCTTTAAGTACAACTTTCCTCTTGTGACTTTATATGCCACACTTGGCAAAGA AGCAGTAGTTCATGGGCTAAATGAATCTGAGGCTTCCTATCTGATTACCAGTGTTGAACTTCTGGAAAGT AAACTTAAGACTGCATTGTTAGATATCAGTTGTGTTAAACATATCATTTATGTGGACAATAAGGCTATCA ATAAAGCAGAGTACCCTGAAGGATTTGAGATTCACAGCATGCAATCAGTAGAAGAGTTGGGATCTAACCC AGAAAACTTGGGCATTCCTCCAAGTAGACCAACGCCTTCAGACATGGCCATTGTTATGTATACTAGTGGT TCTACTGGCCGACCTAAGGGAGTGATGATGCATCATAGCAATTTGATAGCTGGAATGACAGGCCAGTGTG AAAGAATACCTGGACTGGGACCGAAGGACACATATATTGGCTACTTGCCTTTGGCTCATGTGCTAGAACT GACAGCAGAGATATCTTGCTTTACCTATGGCTGCAGGATTGGATATTCTTCTCCGCTTACACTCTCTGAC CAGTCCAGCAAAATTAAAAAAGGAAGCAAAGGAGACTGTACTGTACTGAAGCCCACACTTATGGCTGCTG TTCCGGAAATCATGGATAGAATTTATAAGAATGTTATGAGCAAAGTCCAAGAGATGAATTATATTCAGAA AACTCTGTTCAAGATAGGGTATGATTACAAATTGGAACAGATCAAAAAGGGATATGATGCACCTCTTTGC AATCTGTTACTGTTTAAAAAGGTCAAGGCCCTGCTGGGAGGGAATGTCCGCATGATGCTGTCTGGAGGGG CCCCGCTATCTCCTCAGACACACCGATTCATGAATGTCTGCTTCTGCTGCCCAATTGGCCAGGGTTATGG ACTGACAGAATCATGTGGTGCTGGGACAGTTACTGAAGTAACTGACTATACTACTGGCAGAGTTGGAGCA CCTCTTATTTGCTGTGAAATTAAGCTAAAAGACTGGCAAGAAGGCGGTTATACAATTAATGACAAGCCAA ACCCCAGAGGTGAAATCGTAATTGGTGGACAGAACATCTCCATGGGATATTTTAAAAATGAAGAGAAAAC AGCAGAAGATTATTCTGTGGATGAAAATGGACAAAGGTGGTTTTGCACTGGTGATATTGGAGAATTCCAT CCCGATGGATGTTTACAGATTATAGATCGTAAGAAAGATCTAGTGAAGTTACAAGCAGGAGAGTATGTAT CTCTTGGGAAAGTAGAAGCTGCACTGAAGAATTGTCCACTTATTGACAACATCTGTGCTTTTGCCAAAAG TGATCAGTCCTATGTGATCAGTTTTGTGGTTCCTAACCAGAAAAGGTTGACACTTTTGGCACAACAGAAA GGGGTAGAAGGAACTTGGGTTGATATCTGCAATAATCCTGCTATGGAAGCTGAAATACTGAAAGAAATTC GAGAAGCTGCAAATGCCATGAAATTGGAGCGATTTGAAATTCCAATCAAGGTTCGATTAAGCCCAGAGCC ATGGACCCCTGAAACTGGTTTGGTAACTGATGCTTTCAAACTGAAAAGGAAGGAGCTGAGGAACCATTAC CTCAAAGACATTGAACGAATGTATGGGGGCAAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG221413 representing NM_022977
Red=Cloning site Green=Tags(s) MKLKLNVLTIILLPVHLLITIYSALIFIPWYFLTNAKKKNAMAKRIKAKPTSDKPGSPYRSVTHFDSLAV IDIPGADTLDKLFDHAVSKFGKKDSLGTREILSEENEMQPNGKVFKKLILGNYKWMNYLEVNRRVNNFGS GLTALGLKPKNTIAIFCETRAEWMIAAQTCFKYNFPLVTLYATLGKEAVVHGLNESEASYLITSVELLES KLKTALLDISCVKHIIYVDNKAINKAEYPEGFEIHSMQSVEELGSNPENLGIPPSRPTPSDMAIVMYTSG STGRPKGVMMHHSNLIAGMTGQCERIPGLGPKDTYIGYLPLAHVLELTAEISCFTYGCRIGYSSPLTLSD QSSKIKKGSKGDCTVLKPTLMAAVPEIMDRIYKNVMSKVQEMNYIQKTLFKIGYDYKLEQIKKGYDAPLC NLLLFKKVKALLGGNVRMMLSGGAPLSPQTHRFMNVCFCCPIGQGYGLTESCGAGTVTEVTDYTTGRVGA PLICCEIKLKDWQEGGYTINDKPNPRGEIVIGGQNISMGYFKNEEKTAEDYSVDENGQRWFCTGDIGEFH PDGCLQIIDRKKDLVKLQAGEYVSLGKVEAALKNCPLIDNICAFAKSDQSYVISFVVPNQKRLTLLAQQK GVEGTWVDICNNPAMEAEILKEIREAANAMKLERFEIPIKVRLSPEPWTPETGLVTDAFKLKRKELRNHY LKDIERMYGGK TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_022977 |
ORF Size | 2133 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_022977.2 |
RefSeq Size | 5356 bp |
RefSeq ORF | 2136 bp |
Locus ID | 2182 |
UniProt ID | O60488 |
Domains | AMP-binding |
Protein Families | Druggable Genome, Transmembrane |
Protein Pathways | Adipocytokine signaling pathway, Fatty acid metabolism, Metabolic pathways, PPAR signaling pathway |
Gene Summary | The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme preferentially utilizes arachidonate as substrate. The absence of this enzyme may contribute to the cognitive disability or Alport syndrome. Alternative splicing of this gene generates multiple transcript variants. [provided by RefSeq, Jan 2016] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC221413 | ACSL4 (Myc-DDK-tagged)-Human acyl-CoA synthetase long-chain family member 4 (ACSL4), transcript variant 2 |
CNY 5,208.00 |
|
RC221413L3 | Lenti ORF clone of Human acyl-CoA synthetase long-chain family member 4 (ACSL4), transcript variant 2, Myc-DDK-tagged |
CNY 7,700.00 |
|
RC221413L4 | Lenti ORF clone of Human acyl-CoA synthetase long-chain family member 4 (ACSL4), transcript variant 2, mGFP tagged |
CNY 7,700.00 |
|
SC109208 | ACSL4 (untagged)-Human acyl-CoA synthetase long-chain family member 4 (ACSL4), transcript variant 2 |
CNY 5,216.00 |