PAG3 (ASAP2) (NM_003887) Human Tagged ORF Clone
CAT#: RG221783
- TrueORF®
ASAP2 (tGFP-tagged) - Human ArfGAP with SH3 domain, ankyrin repeat and PH domain 2 (ASAP2), transcript variant 1
ORF Plasmid: DDK
"NM_003887" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 8,608.00
CNY 1,000.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | AMAP2; CENTB3; DDEF2; PAG3; PAP; Pap-alpha; SHAG1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG221783 representing NM_003887
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGGACCAGATCTCCGTGTCGGAATTCGTGGCCGAGACCCATGAGGACTACAAGGCGCCCACGGCCT CCAGCTTCACCACCCGCACGGCGCAGTGCCGGAACACTGTGGCGGCCATCGAGGAGGCTTTGGACGTGGA CCGGATGGTTCTTTACAAAATGAAGAAATCCGTGAAAGCAATCAACAGCTCTGGGCTGGCTCACGTGGAA AATGAAGAGCAGTACACCCAGGCTCTGGAGAAGTTTGGCGGCAACTGTGTATGCAGAGATGACCCAGATT TAGGAAGTGCGTTCCTGAAGTTCTCAGTGTTTACAAAGGAGTTGACAGCACTTTTCAAAAACCTGATTCA GAATATGAACAACATAATCTCCTTCCCTTTGGACAGTTTGCTGAAGGGGGACCTGAAAGGAGTGAAAGGG GATCTGAAAAAGCCTTTTGATAAAGCTTGGAAGGACTATGAAACAAAAATAACCAAGATAGAAAAGGAGA AAAAGGAACACGCCAAGCTCCATGGGATGATTCGGACTGAAATAAGCGGAGCGGAAATTGCCGAAGAGAT GGAAAAGGAGAGGCGCTTCTTCCAGCTACAGATGTGCGAGTATCTGCTGAAGGTCAACGAAATCAAGATT AAAAAGGGAGTAGATTTACTTCAGAATCTGATCAAATACTTTCATGCCCAATGCAATTTTTTTCAGGATG GACTCAAAGCCGTGGAAAGCCTCAAACCTTCCATTGAAACGCTGTCTACGGATCTTCACACGATCAAACA GGCCCAGGATGAAGAAAGAAGGCAGTTGATACAGCTTCGAGATATTTTGAAATCCGCATTGCAGGTTGAA CAGAAAGAGTCTAGGAGAGACTCCCAAATTCGTCAGAGCACAGCTTATAGCTTACATCAGCCTCAGGGAA ACAAGGAACATGGGACCGAGCGGAACGGCAGCCTCTACAAGAAGAGTGACGGGATCCGAAAAGTGTGGCA GAAAAGGAAATGTTCAGTTAAAAATGGTTTTCTGACCATATCCCATGGTACCGCTAACCGGCCTCCTGCA AAGCTCAACCTGCTAACCTGCCAGGTGAAGACCAACCCTGAGGAGAAGAAGTGCTTTGACCTTATTTCAC ATGACAGAACTTACCACTTTCAAGCTGAAGATGAACAGGAATGTCAAATATGGATGTCTGTGCTGCAAAA TAGCAAAGAAGAAGCTTTAAACAATGCATTTAAGGGGGATGACAATACTGGAGAAAATAACATCGTCCAA GAACTGACAAAGGAGATCATCTCAGAAGTGCAGAGGATGACGGGCAATGACGTCTGCTGTGACTGTGGGG CGCCAGATCCTACATGGCTTTCCACCAACCTGGGCATCCTGACCTGCATCGAGTGTTCCGGAATCCACCG AGAGCTGGGGGTTCATTATTCCAGGATGCAGTCCCTGACCTTAGATGTACTGGGAACATCTGAGCTGCTG CTCGCCAAGAATATTGGGAATGCAGGCTTTAATGAGATCATGGAATGTTGCCTACCAGCTGAGGACTCAG TCAAACCCAACCCAGGCAGCGACATGAATGCAAGAAAGGACTACATCACAGCCAAGTACATCGAGAGGAG ATACGCAAGGAAGAAGCACGCGGATAACGCGGCGAAGCTTCACAGTCTTTGCGAGGCCGTCAAAACGAGA GATATTTTTGGATTGCTCCAAGCTTATGCTGATGGTGTGGATCTTACGGAAAAAATCCCACTGGCCAACG GACATGAGCCGGATGAAACGGCCCTCCACCTTGCAGTCAGATCCGTGGATCGAACCTCTCTTCACATTGT AGACTTTTTAGTTCAGAACAGTGGGAACCTGGATAAACAGACAGGGAAAGGCAGCACAGCCCTGCACTAC TGCTGCCTGACCGACAATGCCGAGTGCCTCAAGTTGCTCCTGCGGGGGAAGGCCTCCATCGAGATAGCAA ACGAGTCAGGAGAGACTCCGCTGGACATTGCCAAGCGCCTCAAGCACGAGCACTGTGAGGAGCTGCTGAC CCAAGCCTTATCTGGAAGATTTAATTCTCACGTTCACGTTGAATATGAATGGCGACTACTCCACGAAGAC CTGGATGAAAGTGATGACGACATGGATGAGAAATTGCAGCCCAGTCCCAACCGGCGGGAAGACCGGCCCA TCAGCTTCTACCAGCTGGGCTCCAACCAGCTTCAGTCTAACGCTGTATCTTTGGCCAGAGATGCTGCAAA CCTTGCCAAGGAGAAGCAGAGGGCTTTCATGCCCAGCATCTTGCAGAATGAGACTTACGGAGCCCTCCTG AGTGGCAGCCCACCTCCCGCCCAGCCTGCAGCCCCCAGCACCACCAGCGCCCCCCCGCTTCCTCCACGGA ATGTTGGCAAAGTTCAGACAGCCTCCTCTGCTAACACCCTGTGGAAGACAAACTCTGTAAGTGTGGACGG TGGAAGCCGGCAGCGATCTTCGTCAGATCCGCCAGCTGTCCATCCACCGCTGCCCCCTCTTCGCGTGACA TCTACCAATCCCCTGACCCCCACGCCGCCCCCACCCGTTGCCAAGACGCCCAGCGTAATGGAAGCCTTGA GCCAGCCGAGCAAGCCTGCCCCGCCTGGGATCTCACAGATCAGGCCCCCACCTCTGCCCCCACAGCCGCC CAGCCGCCTCCCGCAGAAGAAGCCTGCGCCGGGGGCTGACAAGTCCACCCCACTGACCAACAAAGGCCAA CCGAGAGGACCTGTGGATCTCTCTGCAACGGAAGCTCTGGGTCCTCTGTCCAATGCTATGGTCCTGCAGC CCCCTGCACCCATGCCTAGGAAGTCGCAGGCAACCAAGTTGAAGCCTAAGCGGGTGAAAGCGCTCTATAA CTGTGTGGCTGACAACCCCGATGAGCTCACCTTCTCCGAGGGGGATGTGATCATCGTGGACGGGGAGGAG GACCAGGAGTGGTGGATTGGCCACATTGATGGAGATCCTGGTCGCAAAGGCGCATTCCCGGTGTCATTTG TGCACTTTATCGCTGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG221783 representing NM_003887
Red=Cloning site Green=Tags(s) MPDQISVSEFVAETHEDYKAPTASSFTTRTAQCRNTVAAIEEALDVDRMVLYKMKKSVKAINSSGLAHVE NEEQYTQALEKFGGNCVCRDDPDLGSAFLKFSVFTKELTALFKNLIQNMNNIISFPLDSLLKGDLKGVKG DLKKPFDKAWKDYETKITKIEKEKKEHAKLHGMIRTEISGAEIAEEMEKERRFFQLQMCEYLLKVNEIKI KKGVDLLQNLIKYFHAQCNFFQDGLKAVESLKPSIETLSTDLHTIKQAQDEERRQLIQLRDILKSALQVE QKESRRDSQIRQSTAYSLHQPQGNKEHGTERNGSLYKKSDGIRKVWQKRKCSVKNGFLTISHGTANRPPA KLNLLTCQVKTNPEEKKCFDLISHDRTYHFQAEDEQECQIWMSVLQNSKEEALNNAFKGDDNTGENNIVQ ELTKEIISEVQRMTGNDVCCDCGAPDPTWLSTNLGILTCIECSGIHRELGVHYSRMQSLTLDVLGTSELL LAKNIGNAGFNEIMECCLPAEDSVKPNPGSDMNARKDYITAKYIERRYARKKHADNAAKLHSLCEAVKTR DIFGLLQAYADGVDLTEKIPLANGHEPDETALHLAVRSVDRTSLHIVDFLVQNSGNLDKQTGKGSTALHY CCLTDNAECLKLLLRGKASIEIANESGETPLDIAKRLKHEHCEELLTQALSGRFNSHVHVEYEWRLLHED LDESDDDMDEKLQPSPNRREDRPISFYQLGSNQLQSNAVSLARDAANLAKEKQRAFMPSILQNETYGALL SGSPPPAQPAAPSTTSAPPLPPRNVGKVQTASSANTLWKTNSVSVDGGSRQRSSSDPPAVHPPLPPLRVT STNPLTPTPPPPVAKTPSVMEALSQPSKPAPPGISQIRPPPLPPQPPSRLPQKKPAPGADKSTPLTNKGQ PRGPVDLSATEALGPLSNAMVLQPPAPMPRKSQATKLKPKRVKALYNCVADNPDELTFSEGDVIIVDGEE DQEWWIGHIDGDPGRKGAFPVSFVHFIAD TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_003887 |
ORF Size | 3027 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_003887.1, NP_003878.1 |
RefSeq Size | 5711 bp |
RefSeq ORF | 3021 bp |
Locus ID | 8853 |
UniProt ID | O43150 |
Domains | ArfGap, SH3, PH, ANK |
Protein Pathways | Endocytosis, Fc gamma R-mediated phagocytosis |
Gene Summary | This gene encodes a multidomain protein containing an N-terminal alpha-helical region with a coiled-coil motif, followed by a pleckstrin homology (PH) domain, an Arf-GAP domain, an ankyrin homology region, a proline-rich region, and a C-terminal Src homology 3 (SH3) domain. The protein localizes in the Golgi apparatus and at the plasma membrane, where it colocalizes with protein tyrosine kinase 2-beta (PYK2). The encoded protein forms a stable complex with PYK2 in vivo. This interaction appears to be mediated by binding of its SH3 domain to the C-terminal proline-rich domain of PYK2. The encoded protein is tyrosine phosphorylated by activated PYK2. It has catalytic activity for class I and II ArfGAPs in vitro, and can bind the class III Arf ARF6 without immediate GAP activity. The encoded protein is believed to function as an ARF GAP that controls ARF-mediated vesicle budding when recruited to Golgi membranes. In addition, it functions as a substrate and downstream target for PYK2 and SRC, a pathway that may be involved in the regulation of vesicular transport. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC221783 | ASAP2 (Myc-DDK-tagged)-Human ArfGAP with SH3 domain, ankyrin repeat and PH domain 2 (ASAP2), transcript variant 1 |
CNY 7,008.00 |
|
RC221783L3 | Lenti ORF clone of Human ArfGAP with SH3 domain, ankyrin repeat and PH domain 2 (ASAP2), transcript variant 1, Myc-DDK-tagged |
CNY 11,970.00 |
|
RC221783L4 | Lenti ORF clone of Human ArfGAP with SH3 domain, ankyrin repeat and PH domain 2 (ASAP2), transcript variant 1, mGFP tagged |
CNY 11,970.00 |
|
SC117694 | ASAP2 (untagged)-Human ArfGAP with SH3 domain, ankyrin repeat and PH domain 2 (ASAP2), transcript variant 1 |
CNY 7,016.00 |