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SH3D20 (ARHGAP27) (NM_199282) Human Tagged ORF Clone
CAT＃: RG222412
- TrueORF®
ARHGAP27 (tGFP-tagged) - Human Rho GTPase activating protein 27 (ARHGAP27), transcript variant 1
ORF Plasmid: DDK
"NM_199282" in other vectors (4)
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CNY 5,680.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CAMGAP1; PP905; SH3D20; SH3P20 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG222412 representing NM_199282
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGCCGGGCCTGAGCCCTGGCAGCCCAGGGGACCCGCGGCCCCCCACTCCCGAGACGGACTACCCCG AGTCGCTGACCAGTTACCCCGAGGAGGACTATTCTCCCGTGGGCTCTTTCGGTGAGCCCGGCCCTACCTC TCCCTTGACCACACCCCCCGGCTGGTCTTGTCATGTCAGCCAGGACAAGCAGATGCTCTACACCAACCAC TTCACTCAGGAGCAGTGGGTGAGGCTGGAGGACCCCCACGGGAAGCCATACTTCTACAATCCAGAGGACT CCTCTGTTCGATGGGAGCTGCCCCAGGTCCCTGTCCCTGCCCCTCGAAGCATCCATAAATCCAGCCAGGA TGGTGACACCCCAGCCCAGGCCAGCCCTCCAGAGGAGAAGGTCCCAGCAGAGCTGGATGAGGTTGGGAGC TGGGAGGAAGTCTCTCCTGCCACAGCTGCTGTGAGGACCAAGACCTTGGACAAGGCAGGGGTGCTCCATC GCACCAAGACGGCAGACAAGGGAAAGCGGCTCCGGAAGAAGCACTGGAGTGCCTCCTGGACTGTGCTGGA GGGTGGCGTCCTGACATTCTTCAAGGACTCAAAGACCTCGGCTGCAGGCGGCCTGAGGCAGCCTTCCAAG TTTTCCACCCCTGAGTACACAGTGGAGCTGAGGGGGGCCACTCTCTCCTGGGCCCCCAAAGACAAATCCA GTAGGAAGAATGTGCTGGAGCTACGGAGCCGAGATGGCTCTGAGTACCTGATCCAGCACGACTCGGAGGC CATCATCAGCACCTGGCATAAGGCCATTGCTCAGGGCATCCAGGAGCTGTCCGCAGAGCTGCCCCCAGAG GAGAGCGAGAGCAGCAGAGTGGACTTCGGGTCGAGCGAGCGCTTGGGAAGCTGGCAGGAGAAAGAGGAGG ACGCGCGACCGAATGCAGCCGCGCCCGCCCTGGGCCCCGTGGGCCTGGAGAGCGACTTGAGCAAGGTCCG GCACAAGCTCCGCAAGTTCCTCCAGAGGCGGCCCACACTGCAGTCGCTGCGGGAGAAGGGCTACATCAAA GACCAGGTGTTCGGCTGCGCGCTGGCCGCGCTGTGTGAGCGCGAGAGGAGCCGGGTGCCACGCTTCGTGC AGCAGTGCATCCGCGCCGTCGAGGCCCGCGGGCTGGACATCGACGGGCTGTACCGCATCAGTGGAAACCT GGCCACCATCCAGAAGCTACGCTATAAGGTGGACCACGATGAGCGCCTTGACCTGGATGACGGGCGCTGG GAGGACGTCCACGTTATCACCGGAGCCCTGAAGCTCTTCTTTCGGGAGCTGCCCGAGCCCCTCTTCCCCT TCTCGCACTTCCGCCAGTTCATTGCGGCCATCAAGTTGCAGGACCAGGCCCGGCGCAGCCGCTGTGTGCG TGACTTGGTGCGCTCGCTGCCCGCTCCCAACCACGACACTCTGCGGATGCTCTTCCAGCACCTCTGCCGG GTGATCGAGCACGGCGAGCAGAACCGCATGTCGGTGCAGAGCGTGGCCATTGTGTTCGGGCCCACGCTGC TGCGGCCCGAGGTGGAAGAGACCAGCATGCCCATGACCATGGTGTTCCAGAACCAGGTGGTGGAGCTCAT CCTGCAGCAGTGCGCGGACATCTTCCCGCCGCAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG222412 representing NM_199282
Red=Cloning site Green=Tags(s) MQPGLSPGSPGDPRPPTPETDYPESLTSYPEEDYSPVGSFGEPGPTSPLTTPPGWSCHVSQDKQMLYTNH FTQEQWVRLEDPHGKPYFYNPEDSSVRWELPQVPVPAPRSIHKSSQDGDTPAQASPPEEKVPAELDEVGS WEEVSPATAAVRTKTLDKAGVLHRTKTADKGKRLRKKHWSASWTVLEGGVLTFFKDSKTSAAGGLRQPSK FSTPEYTVELRGATLSWAPKDKSSRKNVLELRSRDGSEYLIQHDSEAIISTWHKAIAQGIQELSAELPPE ESESSRVDFGSSERLGSWQEKEEDARPNAAAPALGPVGLESDLSKVRHKLRKFLQRRPTLQSLREKGYIK DQVFGCALAALCERERSRVPRFVQQCIRAVEARGLDIDGLYRISGNLATIQKLRYKVDHDERLDLDDGRW EDVHVITGALKLFFRELPEPLFPFSHFRQFIAAIKLQDQARRSRCVRDLVRSLPAPNHDTLRMLFQHLCR VIEHGEQNRMSVQSVAIVFGPTLLRPEVEETSMPMTMVFQNQVVELILQQCADIFPPH TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_199282 |
| ORF Size | 1644 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_199282.3 |
| RefSeq Size | 3628 bp |
| RefSeq ORF | 1647 bp |
| Locus ID | 201176 |
| UniProt ID | Q6ZUM4 |
| Gene Summary | This gene encodes a member of a large family of proteins that activate Rho-type guanosine triphosphate (GTP) metabolizing enzymes. The encoded protein may pay a role in clathrin-mediated endocytosis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2013] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222412 | ARHGAP27 (Myc-DDK-tagged)-Human Rho GTPase activating protein 27 (ARHGAP27), transcript variant 1 |
CNY 4,080.00 |
|
| RC222412L3 | Lenti ORF clone of Human Rho GTPase activating protein 27 (ARHGAP27), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC222412L4 | Lenti ORF clone of Human Rho GTPase activating protein 27 (ARHGAP27), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| SC310278 | ARHGAP27 (untagged)-Human Rho GTPase activating protein 27 (ARHGAP27), transcript variant 1 |
CNY 7,220.00 |
