SP5 (NM_001003845) Human Tagged ORF Clone

CAT#: RG222663

  • TrueORF®

SP5 (tGFP-tagged) - Human Sp5 transcription factor (SP5)

ORF Plasmid: DDK tGFP



  "NM_001003845" in other vectors (6)

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CNY 4,370.00


货期*
2周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag TurboGFP
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RG222663 representing NM_001003845
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGCGGTGGCCGTCCTCCGGAACGACTCGCTGCAGGCCTTTCTCCAGGACCGCACCCCCAGCGCCT
CCCCGGACCTGGGCAAGCACTCGCCCCTGGCATTGCTGGCCGCCACCTGTAGCCGCATCGGCCAGCCGGG
CGCGGCGGCGCCCCCGGACTTCCTGCAGGTGCCCTACGACCCCGCGCTGGGCTCACCCTCCAGGCTCTTC
CACCCGTGGACCGCCGACATGCCGGCGCACTCGCCAGGCGCACTGCCGCCCCCGCATCCCAGCTTGGGGC
TGACGCCGCAGAAGACGCACCTGCAGCCGTCCTTCGGGGCTGCGCACGAGCTTCCCCTTACACCCCCCGC
CGACCCCTCGTACCCCTACGAGTTCTCGCCGGTCAAGATGCTGCCCTCGAGCATGGCGGCTCTGCCCGCC
AGCTGCGCGCCCGCCTACGTGCCCTACGCGGCGCAGGCCGCGCTGCCGCCAGGCTACTCCAACCTGCTGC
CTCCGCCGCCGCCACCGCCCCCGCCGCCCACCTGCCGCCAGTTGTCACCCAACCCGGCCCCCGACGACCT
CCCGTGGTGGAGCATCCCGCAGGCGGGCGCCGGGCCGGGGGCCTCCGGGGTTCCGGGAAGCGGCCTCTCC
GGCGCCTGTGCCGGGGCCCCCCACGCGCCCCGCTTCCCCGCCTCTGCGGCCGCTGCTGCTGCGGCCGCCG
CCGCCCTACAAAGAGGCCTGGTGTTGGGCCCGTCGGACTTTGCGCAGTACCAGAGCCAGATCGCCGCGCT
GCTGCAGACCAAGGCCCCCCTGGCGGCCACGGCCAGGAGGTGCCGCCGCTGCCGCTGTCCCAACTGCCAG
GCGGCGGGCGGCGCCCCCGAGGCGGAGCCGGGGAAGAAGAAGCAGCACGTGTGCCACGTGCCGGGCTGCG
GCAAGGTGTACGGGAAGACGTCGCACCTGAAGGCGCACCTGCGCTGGCACACGGGCGAGCGACCCTTCGT
GTGCAACTGGCTCTTCTGCGGGAAGAGCTTCACGCGCTCGGACGAGCTGCAGCGGCACCTGCGGACTCAC
ACGGGCGAGAAGCGCTTTGCCTGTCCCGAGTGCGGCAAGCGCTTCATGCGCAGCGACCACCTCGCGAAGC
ACGTCAAGACTCACCAGAATAAGAAGCTCAAAGTCGCTGAGGCCGGGGTTAAGCGGGAGGACGCGCGGGA
CCTG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG222663 representing NM_001003845
Red=Cloning site Green=Tags(s)

MAAVAVLRNDSLQAFLQDRTPSASPDLGKHSPLALLAATCSRIGQPGAAAPPDFLQVPYDPALGSPSRLF
HPWTADMPAHSPGALPPPHPSLGLTPQKTHLQPSFGAAHELPLTPPADPSYPYEFSPVKMLPSSMAALPA
SCAPAYVPYAAQAALPPGYSNLLPPPPPPPPPPTCRQLSPNPAPDDLPWWSIPQAGAGPGASGVPGSGLS
GACAGAPHAPRFPASAAAAAAAAAALQRGLVLGPSDFAQYQSQIAALLQTKAPLAATARRCRRCRCPNCQ
AAGGAPEAEPGKKKQHVCHVPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFCGKSFTRSDELQRHLRTH
TGEKRFACPECGKRFMRSDHLAKHVKTHQNKKLKVAEAGVKREDARDL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001003845
ORF Size 1194 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001003845.3
RefSeq Size 1941 bp
RefSeq ORF 1197 bp
Locus ID 389058
UniProt ID Q6BEB4
Protein Families Transcription Factors
Gene Summary Binds to GC boxes promoters elements. Probable transcriptional activator that has a role in the coordination of changes in transcription required to generate pattern in the developing embryo (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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