CD57 (B3GAT1) (NM_018644) Human Tagged ORF Clone
CAT#: RG222914
- TrueORF®
B3GAT1 (tGFP-tagged) - Human beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P) (B3GAT1), transcript variant 1
ORF Plasmid: DDK
"NM_018644" in other vectors (4)
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CNY 5,256.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | CD57; GLCATP; GLCUATP; HNK1; LEU7; NK-1; NK1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG222914 representing NM_018644
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGAAGAGACGGGACATCCTAGCGATCGTCCTCATCGTGCTGCCCTGGACTCTGCTCATCACTGTCT GGCACCAGAGCACCCTCGCACCCCTGCTCGCGGTACATAAGGATGAGGGCAGTGACCCCCGACGCGAAAC GCCGCCCGGCGCCGACCCCAGGGAGTACTGCACGTCTGACCGCGACATCGTGGAGGTGGTGCGCACCGAG TACGTGTACACGCGGCCCCCGCCATGGTCCGACACGCTGCCCACCATCCACGTGGTGACGCCCACCTACA GCCGCCCGGTGCAGAAGGCCGAGCTGACGCGCATGGCCAACACGCTGCTGCACGTGCCCAACCTCCACTG GCTGGTGGTGGAGGATGCGCCGCGCCGGACGCCGCTGACCGCGCGCCTGCTGCGCGACACCGGCCTCAAC TACACGCACCTGCACGTGGAGACGCCCCGCAACTACAAGCTGCGCGGAGACGCCCGCGACCCACGCATCC CGCGGGGCACCATGCAGCGCAACCTGGCCCTGCGCTGGCTGCGCGAGACCTTCCCGCGCAACTCCAGCCA GCCTGGCGTGGTCTACTTCGCCGACGACGACAACACCTACAGCCTGGAGCTCTTCGAAGAGATGCGCAGC ACCAGGAGGGTGTCCGTGTGGCCCGTCGCCTTCGTGGGTGGCCTGCGGTACGAGGCCCCACGGGTGAACG GGGCAGGGAAGGTGGTCGGCTGGAAGACGGTGTTTGACCCCCACCGGCCATTTGCAATAGACATGGCTGG ATTTGCCGTCAACCTGCGGCTCATTCTGCAGCGAAGCCAGGCCTACTTCAAGCTGCGAGGTGTGAAGGGA GGCTACCAGGAAAGCAGCCTCCTTCGAGAACTTGTCACCCTCAACGACCTGGAGCCCAAGGCAGCCAACT GCACCAAGATCCTGGTGTGGCACACACGGACAGAGAAGCCAGTGCTGGTGAATGAGGGCAAGAAGGGCTT CACTGACCCCTCGGTGGAGATC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG222914 representing NM_018644
Red=Cloning site Green=Tags(s) MPKRRDILAIVLIVLPWTLLITVWHQSTLAPLLAVHKDEGSDPRRETPPGADPREYCTSDRDIVEVVRTE YVYTRPPPWSDTLPTIHVVTPTYSRPVQKAELTRMANTLLHVPNLHWLVVEDAPRRTPLTARLLRDTGLN YTHLHVETPRNYKLRGDARDPRIPRGTMQRNLALRWLRETFPRNSSQPGVVYFADDDNTYSLELFEEMRS TRRVSVWPVAFVGGLRYEAPRVNGAGKVVGWKTVFDPHRPFAIDMAGFAVNLRLILQRSQAYFKLRGVKG GYQESSLLRELVTLNDLEPKAANCTKILVWHTRTEKPVLVNEGKKGFTDPSVEI TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_018644 |
ORF Size | 1002 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_018644.3 |
RefSeq Size | 3546 bp |
RefSeq ORF | 1005 bp |
Locus ID | 27087 |
UniProt ID | Q9P2W7 |
Domains | Glyco_transf_43 |
Protein Families | Transmembrane |
Protein Pathways | Chondroitin sulfate biosynthesis, Heparan sulfate biosynthesis, Metabolic pathways |
Gene Summary | The protein encoded by this gene is a member of the glucuronyltransferase gene family. These enzymes exhibit strict acceptor specificity, recognizing nonreducing terminal sugars and their anomeric linkages. This gene product functions as the key enzyme in a glucuronyl transfer reaction during the biosynthesis of the carbohydrate epitope HNK-1 (human natural killer-1, also known as CD57 and LEU7). Alternate transcriptional splice variants have been characterized. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC222914 | B3GAT1 (Myc-DDK-tagged)-Human beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P) (B3GAT1), transcript variant 1 |
CNY 3,656.00 |
|
RC222914L3 | Lenti ORF clone of Human beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P) (B3GAT1), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC222914L4 | Lenti ORF clone of Human beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P) (B3GAT1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
SC110387 | B3GAT1 (untagged)-Human beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P) (B3GAT1), transcript variant 1 |
CNY 3,656.00 |