EHM2 (EPB41L4B) (NM_018424) Human Tagged ORF Clone
CAT#: RG223085
- TrueORF®
EPB41L4B (tGFP-tagged) - Human erythrocyte membrane protein band 4.1 like 4B (EPB41L4B), transcript variant 1
ORF Plasmid: DDK
"NM_018424" in other vectors (4)
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CNY 4,940.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | CG1; EHM2; LULU2 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG223085 representing NM_018424
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGCGGTTCCTGCGCCGGACCTTTGGCCGCCGCTCCATGCAGCGCTACGCGCGGGGCGCGGCGGGGC GCGGGGCCGCCGGGCTGGGGGACGAGCGCGATGGGGGGCCACGGGGGGGCCCGGCCGCCGCCGCCTCCTC CTCGGCGCTGCCCGCCGCGCCCGGGGGCAGCGTGTTCCCGGCGGGCGGCGGGCCCCTGCTCACCGGCGGC GCGGCCGTGCACATCTCCGCCGCCGGCGCCGCCAAGGCCACCCTCTACTGCCGCGTCTTCCTGCTCGACG GGACCGAAGTGAGCGTGGACCTGCCGAAACATGCCAAAGGCCAGGATTTGTTTGATCAGATTGTGTACCA CTTGGACCTTGTGGAAACAGATTACTTTGGCCTCCAGTTCCTCGACTCTGCCCAGGTTGCGCACTGGCTG GATCATGCCAAACCCATAAAAAAGCAGATGAAAATTGGACCTGCTTATGCTTTACACTTTCGAGTTAAAT ACTATTCTTCAGAACCAAACAACCTTCGTGAGGAGTTTACAAGGTACCTGTTTGTTTTACAACTCAGGCA TGACATTCTTTCTGGAAAATTGAAATGCCCTTATGAAACAGCTGTGGAATTAGCTGCTCTCTGTCTACAA GCGGAGCTTGGGGAGTGCGAGCTTCCAGAACACACACCAGAGCTTGTGTCTGAGTTTCGGTTCATTCCAA ATCAGACAGAAGCAATGGAATTTGATATCTTCCAGAGATGGAAAGAGTGCAGGGGAAAGAGCCCTGCCCA GGCGGAACTCTCCTATCTGAATAAAGCGAAGTGGCTGGAAATGTATGGGGTAGACATGCACGTTGTCAGG GGAAGAGATGGCTGTGAATATTCTCTTGGACTGACCCCGACAGGCATATTAATCTTTGAAGGAGCTAACA AAATAGGCTTATTCTTTTGGCCTAAAATTACCAAAATGGATTTTAAAAAGAGCAAATTGACACTCGTGGT GGTCGAGGATGATGATCAGGGACGTGAGCAAGAGCACACGTTTGTGTTCCGGTTAGACAGTGCCAGGACC TGCAAACACCTTTGGAAGTGTGCAGTTGAGCACCACGCATTCTTCCGACTGCGGACGCCAGGAAACAGCA AATCCAATAGATCCGACTTTATCAGGCTGGGCTCTCGCTTCAGATTCAGTGGGCGGACAGAATATCAAGC TACACATGGCTCCAGGTTACGAAGAACCAGCACCTTTGAGAGGAAGCCTAGTAAACGTTATCCATCCCGG AGACATTCAACGTTCAAAGCAAGCAACCCAGTGATAGCAGCCCAGCTCTGCTCTAAAACAAATCCAGAAG TCCATAATTACCAGCCTCAATATCATCCTAATATCCATCCCAGCCAGCCCCGGTGGCATCCTCACTCTCC AAATGTCAGGCCATCCTTTCAGGATGACAGGTCGCATTGGAAAGCATCGGCCAGTGGAGATGACAGCCAT TTTGATTATGTCCACGACCAGAACCAGAAGAACTTAGGAGGGATGCAAAGTATGATGTATCGAGATAAAC TCATGACTGCACTT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG223085 representing NM_018424
Red=Cloning site Green=Tags(s) MLRFLRRTFGRRSMQRYARGAAGRGAAGLGDERDGGPRGGPAAAASSSALPAAPGGSVFPAGGGPLLTGG AAVHISAAGAAKATLYCRVFLLDGTEVSVDLPKHAKGQDLFDQIVYHLDLVETDYFGLQFLDSAQVAHWL DHAKPIKKQMKIGPAYALHFRVKYYSSEPNNLREEFTRYLFVLQLRHDILSGKLKCPYETAVELAALCLQ AELGECELPEHTPELVSEFRFIPNQTEAMEFDIFQRWKECRGKSPAQAELSYLNKAKWLEMYGVDMHVVR GRDGCEYSLGLTPTGILIFEGANKIGLFFWPKITKMDFKKSKLTLVVVEDDDQGREQEHTFVFRLDSART CKHLWKCAVEHHAFFRLRTPGNSKSNRSDFIRLGSRFRFSGRTEYQATHGSRLRRTSTFERKPSKRYPSR RHSTFKASNPVIAAQLCSKTNPEVHNYQPQYHPNIHPSQPRWHPHSPNVRPSFQDDRSHWKASASGDDSH FDYVHDQNQKNLGGMQSMMYRDKLMTAL TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_018424 |
ORF Size | 1554 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_018424.3, NP_060894.2 |
RefSeq Size | 3783 bp |
RefSeq ORF | 1557 bp |
Locus ID | 54566 |
UniProt ID | Q9H329 |
Gene Summary | Up-regulates the activity of the Rho guanine nucleotide exchange factor ARHGEF18 (By similarity). Involved in the regulation of the circumferential actomyosin belt in epithelial cells (PubMed:22006950). Promotes cellular adhesion, migration and motility in vitro and may play a role in wound healing (PubMed:23664528). May have a role in mediating cytoskeletal changes associated with steroid-induced cell differentiation (PubMed:14521927).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC223085 | EPB41L4B (Myc-DDK-tagged)-Human erythrocyte membrane protein band 4.1 like 4B (EPB41L4B), transcript variant 1 |
CNY 3,856.00 |
|
RC223085L3 | Lenti-ORF clone of EPB41L4B (Myc-DDK-tagged)-Human erythrocyte membrane protein band 4.1 like 4B (EPB41L4B), transcript variant 1 |
CNY 6,370.00 |
|
RC223085L4 | Lenti-ORF clone of EPB41L4B (mGFP-tagged)-Human erythrocyte membrane protein band 4.1 like 4B (EPB41L4B), transcript variant 1 |
CNY 6,370.00 |
|
SC317872 | EPB41L4B (untagged)-Human erythrocyte membrane protein band 4.1 like 4B (EPB41L4B), transcript variant 1 |
CNY 8,360.00 |