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ADHFE1 (NM_144650) Human Tagged ORF Clone
CAT＃: RG223298
- TrueORF®
ADHFE1 (tGFP-tagged) - Human alcohol dehydrogenase, iron containing, 1 (ADHFE1), nuclear gene encoding mitochondrial protein
ORF Plasmid: DDK
"NM_144650" in other vectors (5)
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CNY 7,088.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | ADH8; HMFT2263; HOT |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG223298 representing NM_144650
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGCTGCCGCCCGAGCCCGGGTCGCGTACTTGCTGAGGCAACTGCAACGCGCAGCGTGCCAGTGCC CAACTCATTCTCATACTTACTCCCAAGCCCCTGGACTTTCACCTTCTGGGAAAACAACAGATTATGCCTT TGAGATGGCTGTTTCAAATATTAGATATGGAGCAGCAGTTACAAAGGAAGTAGGAATGGACCTAAAAAAC ATGGGTGCTAAAAATGTGTGCTTGATGACAGACAAGAACCTCTCCAAGCTCCCTCCTGTGCAAGTAGCTA TGGATTCCCTAGTGAAGAATGGCATCCCCTTTACGGTTTATGATAATGTGAGAGTGGAACCAACGGATTC AAGCTTCATGGAAGCTATTGAGTTTGCCCAAAAGGGAGCTTTTGATGCCTATGTTGCTGTCGGTGGTGGC TCTACCATGGACACCTGTAAGGCTGCTAATCTGTATGCATCCAGCCCTCATTCTGATTTCCTAGATTATG TCAGTGCCCCCATTGGCAAGGGAAAGCCTGTGTCTGTGCCTCTTAAGCCTCTGATTGCAGTGCCAACTAC CTCAGGAACCGGGAGTGAAACTACTGGGGTTGCCATTTTTGACTATGAACACTTGAAAGTAAAAATTGGC ATCACTTCGAGAGCCATCAAACCCACACTGGGACTGATTGATCCTCTGCACACCCTCCACATGCCTGCCC GAGTGGTCGCCAACAGTGGCTTTGATGTGCTTTGCCATGCCCTGGAGTCATACACCACCCTGCCCTACCA CCTGCGGAGCCCCTGCCCTTCAAATCCCATCACACGGCCTGCGTACCAGGGCAGCAACCCAATCAGTGAC ATTTGGGCTATCCACGCGCTGCGGATCGTGGCTAAGTATCTGAAGAGGGCTGTCAGAAATCCCGATGATC TTGAAGCAAGGTCTCATATGCACTTGGCAAGTGCTTTTGCTGGCATCGGCTTTGGAAATGCTGGTGTTCA TCTGTGCCATGGAATGTCTTACCCAATTTCAGGTTTAGTGAAGATGTATAAAGCAAAGGATTACAATGTG GATCACCCACTGGTGCCCCATGGCCTTTCTGTGGTGCTCACGTCCCCAGCGGTGTTCACTTTCACGGCCC AGATGTTTCCAGAGCGACACCTGGAGATGGCAGAAATACTGGGAGCCGACACCCGCACTGCCAGGATCCA AGATGCAGGGCTGGTGTTGGCAGACACGCTCCGGAAATTCTTATTCGATCTGGATGTTGATGATGGCCTA GCAGCTGTTGGTTACTCCAAAGCTGATATCCCCGCACTAGTGAAAGGAACGCTGCCCCAGGAAAGGGTCA CCAAGCTTGCACCCTGTCCCCAGTCAGAAGAGGATCTGGCTGCTCTGTTTGAAGCTTCAATGAAACTGTA T ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG223298 representing NM_144650
Red=Cloning site Green=Tags(s) MAAAARARVAYLLRQLQRAACQCPTHSHTYSQAPGLSPSGKTTDYAFEMAVSNIRYGAAVTKEVGMDLKN MGAKNVCLMTDKNLSKLPPVQVAMDSLVKNGIPFTVYDNVRVEPTDSSFMEAIEFAQKGAFDAYVAVGGG STMDTCKAANLYASSPHSDFLDYVSAPIGKGKPVSVPLKPLIAVPTTSGTGSETTGVAIFDYEHLKVKIG ITSRAIKPTLGLIDPLHTLHMPARVVANSGFDVLCHALESYTTLPYHLRSPCPSNPITRPAYQGSNPISD IWAIHALRIVAKYLKRAVRNPDDLEARSHMHLASAFAGIGFGNAGVHLCHGMSYPISGLVKMYKAKDYNV DHPLVPHGLSVVLTSPAVFTFTAQMFPERHLEMAEILGADTRTARIQDAGLVLADTLRKFLFDLDVDDGL AAVGYSKADIPALVKGTLPQERVTKLAPCPQSEEDLAALFEASMKLY TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_144650 |
| ORF Size | 1401 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_144650.3 |
| RefSeq Size | 1907 bp |
| RefSeq ORF | 1404 bp |
| Locus ID | 137872 |
| UniProt ID | Q8IWW8 |
| Domains | Fe-ADH |
| Gene Summary | The ADHFE1 gene encodes hydroxyacid-oxoacid transhydrogenase (EC 1.1.99.24), which is responsible for the oxidation of 4-hydroxybutyrate in mammalian tissues (Kardon et al., 2006 [PubMed 16616524]).[supplied by OMIM, Mar 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223298 | ADHFE1 (Myc-DDK-tagged)-Human alcohol dehydrogenase, iron containing, 1 (ADHFE1), nuclear gene encoding mitochondrial protein |
CNY 5,488.00 |
|
| RC223298L3 | Lenti-ORF clone of ADHFE1 (Myc-DDK-tagged)-Human alcohol dehydrogenase, iron containing, 1 (ADHFE1), nuclear gene encoding mitochondrial protein |
CNY 7,888.00 |
|
| RC223298L4 | Lenti-ORF clone of ADHFE1 (mGFP-tagged)-Human alcohol dehydrogenase, iron containing, 1 (ADHFE1), nuclear gene encoding mitochondrial protein |
CNY 5,990.00 |
|
| SC120771 | ADHFE1 (untagged)-Human alcohol dehydrogenase, iron containing, 1 (ADHFE1), nuclear gene encoding mitochondrial protein |
CNY 5,488.00 |
|
| SC317769 | ADHFE1 (untagged)-Human alcohol dehydrogenase, iron containing, 1 (ADHFE1), nuclear gene encoding mitochondrial protein |
CNY 5,488.00 |
