COPG2 (NM_012133) Human Tagged ORF Clone
CAT#: RG223372
- TrueORF®
COPG2 (tGFP-tagged) - Human coatomer protein complex, subunit gamma 2 (COPG2)
ORF Plasmid: DDK
"NM_012133" in other vectors (6)
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CNY 10,170.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | 2-COP; gamma-2-COP |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG223372 representing NM_012133
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATTAAAAAATTCGACAAGAAGGACGAGGAGTCTGGTAGTGGCTCCAATCCTTTCCAGCATCTGGAGA AGAGTGCTGTTTTACAGGAGGCTCGTATATTCAATGAAACTCCAATCAATCCAAGAAGATGTTTGCATAT TCTTACAAAGATTCTTTACTTACTGAACCAGGGTGAACACTTTGGAACAACGGAAGCTACAGAAGCCTTC TTTGCAATGACGCGATTGTTTCAATCTAATGATCAAACATTGAGGAGAATGTGCTACCTTACCATCAAAG AAATGGCTACCATCTCTGAGGATGTGATAATTGTCACAAGCAGTCTGACTAAAGACATGACTGGAAAAGA AGATGTATACCGAGGCCCGGCCATCAGAGCTCTCTGCAGGATCACCGATGGAACAATGTTGCAAGCCATT GAAAGATACATGAAGCAGGCCATTGTGGATAAAGTTTCCAGTGTATCCAGTTCAGCACTGGTATCTTCCC TGCACATGATGAAGATAAGCTATGATGTGGTTAAGCGCTGGATCAATGAAGCCCAAGAAGCTGCATCAAG TGATAATATTATGGTCCAGTACCATGCATTGGGAGTCCTGTATCACCTTAGAAAGAATGATCGACTTGCT GTTTCCAAGATGTTGAATAAGTTTACTAAATCTGGTCTCAAGTCACAGTTTGCTTACTGCATGCTGATCC GAATTGCCAGTCGCTTACTAAAAGAAACTGAGGATGGGCATGAAAGTCCACTGTTTGATTTCATTGAGAG CTGCTTGCGAAATAAACATGAAATGGTTATTTATGAAGCTGCTTCAGCTATCATCCATCTTCCTAACTGC ACTGCAAGAGAGTTGGCACCTGCTGTTTCAGTTCTTCAACTTTTCTGTAGTTCTCCTAAGCCAGCCTTGA GATATGCAGCTGTGAGGACCTTGAACAAGGTGGCAATGAAGCACCCCTCTGCTGTTACTGCCTGCAATCT GGACTTAGAAAACTTAATCACAGACTCAAACAGAAGCATTGCTACCTTAGCCATTACTACACTCCTCAAA ACAGGAAGTGAGAGCAGTGTGGACCGGCTCATGAAGCAGATATCTTCTTTTGTGTCTGAAATCTCAGATG AGTTCAAGGTGGTGGTTGTACAGGCAATTAGTGCTCTCTGTCAGAAATACCCTCGAAAGCACAGTGTCAT GATGACTTTCCTCTCCAACATGCTCCGAGATGATGGAGGCTTTGAGTACAAGCGGGCCATTGTGGACTGT ATAATCAGCATTGTGGAAGAGAACCCTGAGAGTAAAGAAGCAGGCCTAGCCCACCTTTGTGAATTCATTG AGGACTGTGAACACACTGTTCTGGCTACTAAGATTCTACACTTGTTGGGCAAAGAGGGCCCTAGAACGCC TGTCCCCTCCAAATATATCCGTTTTATTTTTAATAGGGTTGTCCTGGAGAATGAGGCTGTCAGAGCTGCT GCTGTGAGTGCTTTGGCTAAATTTGGGGCTCAGAATGAGAGTCTTCTCCCAAGCATCCTTGTACTCTTAC AGAGGTGTATGATGGATACTGATGACGAGGTACGAGACAGAGCTACCTTCTATCTGAATGTGCTGCAGCA GAGGCAGATGGCACTAAATGCCACATATATCTTTAATGGTTTGACGGTCTCTGTACCAGGGATGGAAAAA GCCTTACACCAGTACACGTTGGAGCCTTCAGAAAAACCGTTTGACATGAAATCAATTCCTCTTGCTATGG CTCCTGTCTTTGAACAGAAAGCAGAAATCACACTTGTGGCTACTAAGCCAGAGAAGTTGGCTCCTTCCAG GCAAGACATTTTCCAAGAACAATTGGCTGCCATTCCTGAGTTTCTGAATATAGGACCCTTGTTCAAGTCT TCTGAGCCTGTTCAACTTACAGAAGCAGAGACAGAATATTTTGTTCGATGTATCAAGCACATGTTTACCA ATCACATCGTGTTCCAGTTTGACTGCACCAACACTCTCAATGACCAGCTGCTGGAAAAAGTGACAGTGCA GATGGAGCCATCAGATTCCTATGAAGTGCTGTCTTGTATCCCAGCCCCCAGCCTTCCTTATAACCAACCA GGAATATGTTACACTCTTGTTCGTTTGCCTGATGATGACCCTACAGCAGTTGCAGGCTCCTTTAGCTGCA CCATGAAGTTTACAGTCCGGGACTGTGACCCTAACACTGGAGTTCCAGATGAGGATGGGTATGATGATGA GTATGTGCTGGAAGATCTCGAAGTGACTGTGTCTGACCATATTCAGAAAGTACTGAAGCCTAACTTTGCT GCTGCTTGGGAAGAGGTGGGAGATACCTTTGAGAAAGAGGAAACCTTTGCCCTCAGTTCTACCAAAACCC TTGAAGAGGCTGTCAACAATATCATCACATTTCTGGGCATGCAGCCATGTGAGAGGTCCGATAAAGTACC TGAGAACAAGAATTCCCATTCGCTCTATCTGGCAGGTATATTCAGAGGTGGCTATGATTTATTGGTGAGG TCCAGGCTGGCCTTAGCCGATGGAGTGACCATGCAGGTGACTGTCAGAAGTAAAGAGAGAACACCTGTAG ATGTTATCTTAGCTTCTGTTGGA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG223372 representing NM_012133
Red=Cloning site Green=Tags(s) MIKKFDKKDEESGSGSNPFQHLEKSAVLQEARIFNETPINPRRCLHILTKILYLLNQGEHFGTTEATEAF FAMTRLFQSNDQTLRRMCYLTIKEMATISEDVIIVTSSLTKDMTGKEDVYRGPAIRALCRITDGTMLQAI ERYMKQAIVDKVSSVSSSALVSSLHMMKISYDVVKRWINEAQEAASSDNIMVQYHALGVLYHLRKNDRLA VSKMLNKFTKSGLKSQFAYCMLIRIASRLLKETEDGHESPLFDFIESCLRNKHEMVIYEAASAIIHLPNC TARELAPAVSVLQLFCSSPKPALRYAAVRTLNKVAMKHPSAVTACNLDLENLITDSNRSIATLAITTLLK TGSESSVDRLMKQISSFVSEISDEFKVVVVQAISALCQKYPRKHSVMMTFLSNMLRDDGGFEYKRAIVDC IISIVEENPESKEAGLAHLCEFIEDCEHTVLATKILHLLGKEGPRTPVPSKYIRFIFNRVVLENEAVRAA AVSALAKFGAQNESLLPSILVLLQRCMMDTDDEVRDRATFYLNVLQQRQMALNATYIFNGLTVSVPGMEK ALHQYTLEPSEKPFDMKSIPLAMAPVFEQKAEITLVATKPEKLAPSRQDIFQEQLAAIPEFLNIGPLFKS SEPVQLTEAETEYFVRCIKHMFTNHIVFQFDCTNTLNDQLLEKVTVQMEPSDSYEVLSCIPAPSLPYNQP GICYTLVRLPDDDPTAVAGSFSCTMKFTVRDCDPNTGVPDEDGYDDEYVLEDLEVTVSDHIQKVLKPNFA AAWEEVGDTFEKEETFALSSTKTLEEAVNNIITFLGMQPCERSDKVPENKNSHSLYLAGIFRGGYDLLVR SRLALADGVTMQVTVRSKERTPVDVILASVG TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_012133 |
ORF Size | 2613 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_012133.3, NP_036265.3 |
RefSeq Size | 3143 bp |
RefSeq ORF | 2616 bp |
Locus ID | 26958 |
UniProt ID | Q9UBF2 |
Domains | Adaptin_N |
Gene Summary | The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC223372 | COPG2 (Myc-DDK-tagged)-Human coatomer protein complex, subunit gamma 2 (COPG2) |
CNY 9,568.00 |
|
RC223372L1 | Lenti ORF clone of Human coatomer protein complex, subunit gamma 2 (COPG2), Myc-DDK-tagged |
CNY 11,120.00 |
|
RC223372L2 | Lenti ORF clone of Human coatomer protein complex, subunit gamma 2 (COPG2), mGFP tagged |
CNY 11,120.00 |
|
RC223372L3 | Lenti ORF clone of Human coatomer protein complex, subunit gamma 2 (COPG2), Myc-DDK-tagged |
CNY 11,120.00 |
|
RC223372L4 | Lenti ORF clone of Human coatomer protein complex, subunit gamma 2 (COPG2), mGFP tagged |
CNY 11,120.00 |
|
SC318347 | COPG2 (untagged)-Human coatomer protein complex, subunit gamma 2 (COPG2) |
CNY 16,820.00 |