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TDRKH (NM_001083963) Human Tagged ORF Clone
CAT＃: RG223958
- TrueORF®
TDRKH (tGFP-tagged) - Human tudor and KH domain containing (TDRKH), transcript variant 2
ORF Plasmid: DDK
"NM_001083963" in other vectors (4)
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CNY 5,040.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | TDRD2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG223958 representing NM_001083963
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTACTGAACGGACTTCTTGGACAAGCCTGTCCACCATTCAGAAAATAGCCCTGGGCCTTGGGATCC CAGCCAGTGCAACAGTTGCCTATATCCTATACCGCAGGTATAGGGAAAGCAGAGAAGAGCGGCTGACATT TGTTGGGGAAGATGACATTGAGATAGAGATGCGGGTTCCCCAGGAGGCTGTGAAACTCATCATTGGCCGG CAAGGAGCCAATATTAAACAGCTGCGGAAACAGACAGGTGCTCGGATTGATGTGGACACAGAGGATGTAG GCGATGAGCGAGTGCTGCTTATCAGTGGTTTTCCTGTTCAGGTGTGCAAGGCCAAAGCAGCAATCCATCA GATCCTGACAGAGAATACCCCAGTGTCTGAGCAGCTTTCAGTTCCCCAGAGATCTGTGGGCAGAATCATA GGGAGAGGCGGCGAGACAATTCGTTCTATCTGTAAGGCATCTGGAGCCAAAATTACCTGTGACAAAGAAT CAGAAGGGACATTACTACTATCAAGACTTATAAAAATCTCAGGAACACAGAAGGAAGTGGCAGCAGCCAA GCATTTGATACTGGAGAAAGTTTCAGAAGATGAAGAACTTCGGAAGAGAATTGCTCATTCTGCAGAAACC AGGGTCCCACGCAAACAGCCAATCAGTGTGAGAAGAGAAGACATGACAGAGCCAGGTGGAGCTGGAGAGC CAGCATTATGGAAAAACACCAGTTCTAGCATGGAGCCGACTGCACCCCTGGTGACTCCTCCACCCAAAGG AGGAGGCGACATGGCTGTGGTAGTGTCAAAGGAAGGTTCCTGGGAGAAACCTAGTGATGACAGCTTTCAG AAGTCTGAAGCCCAGGCCATCCCAGAGATGCCCATGTTTGAAATCCCCAGTCCTGACTTCAGTTTTCATG CTGATGAGTACCTAGAAGTCTACGTTTCTGCTTCTGAGCACCCTAACCACTTCTGGATCCAGATCGTTGG CTCCCGCAGCCTGCAATTGGATAAGCTTGTCAATGAGATGACCCAGCACTATGAGAATAGTGTGCCTGAA GACTTGACTGTGCATGTAGGAGACATTGTAGCAGCACCTTTACCTACAAATGGTTCCTGGTATCGAGCCC GGGTCCTCGGCACCTTGGAGAATGGGAACTTGGACCTCTATTTTGTTGACTTTGGAGATAATGGAGATTG CCCACTGAAGGACCTCAGGGCTCTCAGGAGTGACTTCCTAAGCCTTCCATTTCAAGCAATAGAATGTAGT CTGGCACGGATTGCTCCCTCAGGTGACCAGTGGGAAGAGGAAGCTTTGGATGAGTTTGATAGACTCACTC ATTGTGCTGACTGGAAGCCTCTGGTAGCCAAGATCTCTAGCTATGTCCAGACTGGGATCTCAACTTGGCC AAAGATCTACTTATATGATACTAGCAATGGGAAGAAACTTGATATTGGGCTAGAATTAGTACACAAAGGA TACGCAATTGAGCTTCCTGAAGACATAGAAGAAAACAGAGCTGTCCCAGACATGTTGAAGGACATGGCCA CAGAAACAGATGCCTCTCTCAGCACGTTGCTCACTGAGACCAAAAAGAGCTCTGGAGAGATAACACATAC CCTGTCCTGCCTCAGCTTATCAGAAGCTGCTTCCATGTCTGGTGATGATAACCTTGAAGATGACTACTTA CTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG223958 representing NM_001083963
Red=Cloning site Green=Tags(s) MSTERTSWTSLSTIQKIALGLGIPASATVAYILYRRYRESREERLTFVGEDDIEIEMRVPQEAVKLIIGR QGANIKQLRKQTGARIDVDTEDVGDERVLLISGFPVQVCKAKAAIHQILTENTPVSEQLSVPQRSVGRII GRGGETIRSICKASGAKITCDKESEGTLLLSRLIKISGTQKEVAAAKHLILEKVSEDEELRKRIAHSAET RVPRKQPISVRREDMTEPGGAGEPALWKNTSSSMEPTAPLVTPPPKGGGDMAVVVSKEGSWEKPSDDSFQ KSEAQAIPEMPMFEIPSPDFSFHADEYLEVYVSASEHPNHFWIQIVGSRSLQLDKLVNEMTQHYENSVPE DLTVHVGDIVAAPLPTNGSWYRARVLGTLENGNLDLYFVDFGDNGDCPLKDLRALRSDFLSLPFQAIECS LARIAPSGDQWEEEALDEFDRLTHCADWKPLVAKISSYVQTGISTWPKIYLYDTSNGKKLDIGLELVHKG YAIELPEDIEENRAVPDMLKDMATETDASLSTLLTETKKSSGEITHTLSCLSLSEAASMSGDDNLEDDYL L TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001083963 |
| ORF Size | 1683 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001083963.1, NP_001077432.1 |
| RefSeq Size | 2829 bp |
| RefSeq ORF | 1686 bp |
| Locus ID | 11022 |
| UniProt ID | Q9Y2W6 |
| Protein Families | Transmembrane |
| Gene Summary | Participates in the primary piRNA biogenesis pathway and is required during spermatogenesis to repress transposable elements and prevent their mobilization, which is essential for the germline integrity. The piRNA metabolic process mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Required for the final steps of primary piRNA biogenesis by participating in the processing of 31-37 nt intermediates into mature piRNAs. May act in pi-bodies and piP-bodies by transferring piRNA precursors or intermediates to or between these granules.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223958 | TDRKH (Myc-DDK-tagged)-Human tudor and KH domain containing (TDRKH), transcript variant 2 |
CNY 4,176.00 |
|
| RC223958L3 | Lenti-ORF clone of TDRKH (Myc-DDK-tagged)-Human tudor and KH domain containing (TDRKH), transcript variant 2 |
CNY 6,460.00 |
|
| RC223958L4 | Lenti-ORF clone of TDRKH (mGFP-tagged)-Human tudor and KH domain containing (TDRKH), transcript variant 2 |
CNY 6,460.00 |
|
| SC316244 | TDRKH (untagged)-Human tudor and KH domain containing (TDRKH), transcript variant 2 |
CNY 9,030.00 |
