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TAF172 (BTAF1) (NM_003972) Human Tagged ORF Clone
CAT＃: RG224324
- TrueORF®
BTAF1 (tGFP-tagged) - Human BTAF1 RNA polymerase II, B-TFIID transcription factor-associated, 170kDa (Mot1 homolog, S. cerevisiae) (BTAF1)
ORF Plasmid: DDK
"NM_003972" in other vectors (2)
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CNY 20,240.00
货期*
8周
规格
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经常一起买 (5)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | MOT1; TAF(II)170; TAF172; TAFII170 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG224324 representing NM_003972
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGTCTCCAGGCTAGATCGCCTTTTTATTTTACTGGATACTGGCACTACTCCTGTTACAAGAAAAG CTGCTGCACAGCAACTTGGAGAAGTGGTGAAGCTTCATCCCCATGAACTAAATAATCTCCTGTCTAAAGT GTTGATATATTTAAGGAGTGCAAATTGGGATACCCGGATTGCAGCAGGACAAGCTGTTGAAGCTATAGTG AAAAATGTACCTGAGTGGAATCCAGTGCCGAGAACCAGACAAGAACCTACTTCCGAAAGTTCTATGGAAG ATTCACCTACTACAGAGCGATTGAATTTTGACAGATTTGATATATGTAGATTGTTACAACATGGTGCATC ACTCCTGGGATCTGCTGGTGCCGAATTTGAAGTCCAAGATGAAAAATCAGGTGAAGTGGATCCTAAAGAG AGGATAGCACGCCAACGAAAATTATTACAGAAGAAACTTGGCCTTAATATGGGAGAAGCAATTGGAATGA GTACTGAAGAACTTTTCAATGATGAGGATTTGGATTATACCCCAACTTCAGCATCCTTTGTTAACAAACA ACCTACTCTTCAGGCAGCTGAATTGATTGACTCAGAGTTTCGAGCAGGAATGAGCAATAGACAAAAGAAC AAAGCTAAAAGAATGGCCAAGTTATTTGCAAAACAGAGATCCAGGGATGCAGTGGAAACTAATGAGAAGA GCAATGATAGCACTGATGGGGAGCCAGAAGAAAAGAGACGGAAAATAGCAAATGTTGTTATTAATCAGTC TGCAAATGATTCCAAAGTCTTGATTGATAATATTCCAGACAGCTCTTCCTTAATTGAAGAGACAAATGAA TGGCCTTTGGAAAGCTTTTGTGAAGAACTTTGCAATGACCTTTTTAATCCCTCCTGGGAGGTTCGACATG GTGCAGGCACTGGACTTAGGGAAATTCTTAAAGCTCATGGGAAAAGTGGTGGTAAAATGGGTGACAGCAC TTTAGAAGAGATGATTCAGCAGCATCAAGAGTGGTTGGAAGACTTGGTTATTAGACTCCTTTGTGTTTTT GCATTAGACAGATTTGGAGACTTTGTTTCTGATGAAGTTGTGGCACCAGTTCGTGAAACTTGTGCTCAAA CATTAGGTGTGGTTTTAAAACACATGAACGAAACAGGAGTTCATAAGACTGTGGATGTGCTGCTAAAATT ACTTACACAAGAACAATGGGAAGTTAGACATGGTGGTCTGCTGGGAATAAAATATGCTTTGGCAGTCCGT CAGGATGTAATTAATACTTTATTGCCTAAAGTTTTAACTAGAATAATTGAAGGACTCCAGGATCTTGATG ATGATGTCAGAGCTGTTGCTGCAGCATCATTAGTGCCTGTAGTAGAAAGCCTTGTCTATCTTCAGACACA AAAGGTGCCCTTCATTATAAATACATTGTGGGATGCTCTTCTGGAATTAGATGATCTAACAGCTTCAACA AATAGTATTATGACTCTCCTTTCATCCTTGTTAACTTACCCTCAGGTCCAACAATGCAGTATTCAGCAGT CACTGACAGTTTTAGTTCCACGTGTCTGGCCTTTTTTGCATCACACTATATCATCAGTTCGAAGAGCAGC ATTGGAAACTCTGTTTACGTTATTATCAACACAGGACCAGAACTCTTCATCTTGGCTTATACCTATACTG CCTGATATGCTCCGACACATTTTTCAGTTCTGTGTTTTGGAAAGCAGCCAGGAAATTCTGGACCTTATTC ACAAGGTTTGGATGGAACTGTTGAGTAAGGCTTCAGTTCAGTATGTGGTAGCAGCTGCTTGCCCATGGAT GGGTGCTTGGCTTTGCTTGATGATGCAGCCTTCTCATTTACCTATCGATTTAAATATGTTGCTAGAAGTA AAAGCTAGAGCCAAGGAAAAAACAGGTGGTAAGGTGCGCCAAGGCCAAAGCCAGAATAAAGAAGTACTTC AGGAGTATATTGCAGGTGCCGACACCATCATGGAAGACCCAGCCACCAGGGATTTTGTAGTTATGCGGGC CAGAATGATGGCAGCCAAGTTGTTAGGAGCACTTTGTTGCTGTATTTGTGATCCAGGTGTAAATGTGGTA ACTCAAGAAATTAAACCAGCTGAATCCCTGGGCCAGTTACTACTCTTCCATTTGAACTCCAAGTCTGCTT TACAGAGGATTAGTGTAGCTTTGGTAATCTGTGAATGGGCTGCTTTACAAAAGGAGTGTAAAGCTGTTAC CTTAGCTGTGCAGCCGCGTTTACTTGATATCCTTTCAGAACATTTATATTATGACGAAATTGCCGTTCCA TTCACACGAATGCAGAATGAATGTAAACAACTTATATCATCATTAGCTGATGTACATATTGAAGTTGGTA ATCGAGTAAACAACAATGTTTTAACAATAGATCAAGCTAGTGACTTGGTCACTACTGTTTTTAATGAAGC CACATCATCTTTCGATTTAAATCCTCAAGTGTTACAACAGTTAGATAGTAAACGACAGCAGGTCCAAATG ACAGTTACAGAGACCAACCAGGAGTGGCAAGTGTTGCAGTTGAGAGTGCATACTTTTGCTGCCTGTGCAG TTGTGAGCTTGCAGCAGCTTCCGGAGAAATTAAATCCTATCATAAAACCATTAATGGAGACAATTAAAAA AGAAGAGAATACACTAGTGCAAAACTATGCAGCTCAGTGCATAGCTAAACTCCTTCAGCAGTGCACAACA AGGACGCCCTGTCCCAATTCAAAAATTATTAAAAACCTCTGTAGCTCACTTTGTGTGGACCCATATCTAA CTCCTTGTGTCACATGTCCAGTACCAACACAAAGTGGCCAGGAAAATTCTAAAGGATCCACCTCAGAAAA AGATGGAATGCACCATACTGTCACCAAGCACAGAGGTATAATTACACTCTACAGGCACCAGAAAGCTGCC TTTGCTATCACAAGTAGGCGAGGTCCTACCCCCAAAGCAGTAAAAGCTCAAATAGCAGATCTTCCTGCAG GAAGTAGTGGAAATATTCTTGTTGAACTTGATGAGGCCCAGAAGCCTTACCTGGTACAACGGAGAGGAGC TGAATTTGCTTTGACAACTATAGTAAAGCATTTTGGTGGTGAAATGGCAGTGAAGTTGCCACATCTCTGG GATGCTATGGTTGGCCCATTGAGGAATACAATCGACATAAATAATTTTGATGGAAAATCCCTCCTGGATA AGGGAGATAGCCCTGCTCAAGAATTGGTGAATTCTCTGCAGGTTTTTGAAACAGCAGCAGCTTCAATGGA TTCTGAGCTTCATCCCTTGTTGGTCCAGCATTTGCCACATCTTTATATGTGCCTTCAATACCCCAGTACT GCAGTGAGGCACATGGCTGCTCGTTGTGTAGGTGTCATGAGCAAAATAGCTACCATGGAAACAATGAATA TTTTTTTGGAGAAGGTTCTTCCGTGGCTGGGAGCAATTGATGACAGTGTCAAACAAGAGGGTGCAATTGA AGCACTTGCCTGTGTAATGGAACAACTAGATGTTGGTATTGTTCCATATATTGTCCTTTTAGTTGTTCCT GTATTAGGAAGAATGAGTGATCAGACAGACAGTGTGAGATTCATGGCCACGCAGTGCTTTGCAACGCTAA TTAGACTCATGCCACTTGAGGCAGGCATTCCAGACCCTCCAAACATGTCAGCAGAATTAATCCAATTGAA AGCCAAGGAGCGACACTTTTTGGAGCAATTGTTAGATGGGAAAAAATTGGAAAATTATAAAATTCCAGTA CCAATCAATGCTGAACTCAGAAAATATCAGCAGGATGGTGTGAACTGGTTAGCATTTCTTAATAAGTATA AACTTCATGGAATTCTGTGTGATGACATGGGTTTAGGAAAAACTTTACAGTCCATCTGCATTCTAGCAGG AGATCATTGTCATAGGGCCCAGGAATATGCAAGATCAAAATTAGCAGAATGTATGCCACTTCCTTCCTTA GTGGTTTGTCCGCCAACATTAACAGGCCATTGGGTGGATGAAGTAGGTAAATTTTGCTCTAGAGAATATC TCAACCCGTTGCATTACACTGGACCTCCCACTGAAAGAATAAGGTTACAGCACCAAGTAAAAAGGCACAA TCTAATAGTGGCTTCATATGATGTTGTGAGGAATGACATAGATTTCTTTAGAAATATTAAATTTAACTAC TGCATTCTTGATGAAGGCCATGTCATCAAAAATGGAAAAACAAAGTTGTCAAAAGCAGTAAAACAACTGA CTGCTAATTATAGGATTATTCTTTCTGGAACACCAATCCAGAACAACGTTTTGGAGCTGTGGTCATTATT TGATTTCCTCATGCCAGGATTTTTGGGTACTGAACGCCAGTTTGCTGCTCGATATGGTAAACCTATATTA GCAAGTAGGGATGCTCGAAGCTCCAGTCGAGAGCAAGAAGCAGGTGTTCTTGCTATGGATGCGCTGCACC GCCAAGTACTACCGTTTCTTTTGAGAAGAATGAAAGAAGATGTTTTGCAGGATCTTCCACCTAAAATTAT TCAAGACTATTATTGTACTCTTAGTCCTCTCCAGGTTCAGCTCTATGAAGATTTTGCTAAGTCTCGTGCC AAGTGTGATGTTGATGAAACAGTTTCTTCAGCTACACTTTCTGAAGAAACTGAAAAACCAAAGCTTAAAG CTACAGGCCACGTATTCCAGGCATTACAGTACTTACGTAAACTGTGCAACCATCCAGCATTAGTCTTAAC ACCTCAACATCCAGAATTCAAGACCACTGCCGAAAAACTGGCAGTTCAGAATTCTTCTCTACATGATATT CAACATGCCCCTAAGCTCTCAGCTTTGAAACAATTGCTGTTGGACTGCGGTTTGGGAAATGGCAGCACTT CCGAGAGTGGCACAGAGTCTGTTGTGGCCCAGCACAGGATACTGATATTCTGTCAGCTGAAAAGCATGCT TGATATAGTAGAGCATGATCTCCTCAAACCTCACTTGCCCTCTGTCACTTATTTGAGATTAGATGGCAGC ATACCTCCTGGTCAGAGGCATTCCATTGTTTCCCGGTTTAATAATGATCCATCTATAGACGTTCTGTTAC TTACCACTCACGTTGGTGGCCTGGGACTTAATTTGACAGGCGCTGACACAGTAGTATTTGTGGAGCATGA CTGGAATCCTATGCGAGATCTACAAGCCATGGACCGGGCCCATCGCATTGGGCAGAAACGTGTGGTTAAC GTATACCGATTGATAACCAGAGGAACATTGGAAGAAAAAATAATGGGGTTGCAGAAATTCAAGATGAACA TAGCGAATACTGTTATTAGCCAAGAGAATTCTAGTTTGCAGAGCATGGGGACTGATCAGCTTCTTGATCT GTTTACTCTTGATAAGGATGGCAAAGCAGAAAAAGCTGACACCTCTACTTCTGGGAAAGCAAGTATGAAA TCAATTCTTGAAAACCTGAGTGATCTTTGGGATCAAGAGCAGTATGATTCAGAGTACAGCCTGGAAAATT TTATGCATTCTCTCAAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG224324 representing NM_003972
Red=Cloning site Green=Tags(s) MAVSRLDRLFILLDTGTTPVTRKAAAQQLGEVVKLHPHELNNLLSKVLIYLRSANWDTRIAAGQAVEAIV KNVPEWNPVPRTRQEPTSESSMEDSPTTERLNFDRFDICRLLQHGASLLGSAGAEFEVQDEKSGEVDPKE RIARQRKLLQKKLGLNMGEAIGMSTEELFNDEDLDYTPTSASFVNKQPTLQAAELIDSEFRAGMSNRQKN KAKRMAKLFAKQRSRDAVETNEKSNDSTDGEPEEKRRKIANVVINQSANDSKVLIDNIPDSSSLIEETNE WPLESFCEELCNDLFNPSWEVRHGAGTGLREILKAHGKSGGKMGDSTLEEMIQQHQEWLEDLVIRLLCVF ALDRFGDFVSDEVVAPVRETCAQTLGVVLKHMNETGVHKTVDVLLKLLTQEQWEVRHGGLLGIKYALAVR QDVINTLLPKVLTRIIEGLQDLDDDVRAVAAASLVPVVESLVYLQTQKVPFIINTLWDALLELDDLTAST NSIMTLLSSLLTYPQVQQCSIQQSLTVLVPRVWPFLHHTISSVRRAALETLFTLLSTQDQNSSSWLIPIL PDMLRHIFQFCVLESSQEILDLIHKVWMELLSKASVQYVVAAACPWMGAWLCLMMQPSHLPIDLNMLLEV KARAKEKTGGKVRQGQSQNKEVLQEYIAGADTIMEDPATRDFVVMRARMMAAKLLGALCCCICDPGVNVV TQEIKPAESLGQLLLFHLNSKSALQRISVALVICEWAALQKECKAVTLAVQPRLLDILSEHLYYDEIAVP FTRMQNECKQLISSLADVHIEVGNRVNNNVLTIDQASDLVTTVFNEATSSFDLNPQVLQQLDSKRQQVQM TVTETNQEWQVLQLRVHTFAACAVVSLQQLPEKLNPIIKPLMETIKKEENTLVQNYAAQCIAKLLQQCTT RTPCPNSKIIKNLCSSLCVDPYLTPCVTCPVPTQSGQENSKGSTSEKDGMHHTVTKHRGIITLYRHQKAA FAITSRRGPTPKAVKAQIADLPAGSSGNILVELDEAQKPYLVQRRGAEFALTTIVKHFGGEMAVKLPHLW DAMVGPLRNTIDINNFDGKSLLDKGDSPAQELVNSLQVFETAAASMDSELHPLLVQHLPHLYMCLQYPST AVRHMAARCVGVMSKIATMETMNIFLEKVLPWLGAIDDSVKQEGAIEALACVMEQLDVGIVPYIVLLVVP VLGRMSDQTDSVRFMATQCFATLIRLMPLEAGIPDPPNMSAELIQLKAKERHFLEQLLDGKKLENYKIPV PINAELRKYQQDGVNWLAFLNKYKLHGILCDDMGLGKTLQSICILAGDHCHRAQEYARSKLAECMPLPSL VVCPPTLTGHWVDEVGKFCSREYLNPLHYTGPPTERIRLQHQVKRHNLIVASYDVVRNDIDFFRNIKFNY CILDEGHVIKNGKTKLSKAVKQLTANYRIILSGTPIQNNVLELWSLFDFLMPGFLGTERQFAARYGKPIL ASRDARSSSREQEAGVLAMDALHRQVLPFLLRRMKEDVLQDLPPKIIQDYYCTLSPLQVQLYEDFAKSRA KCDVDETVSSATLSEETEKPKLKATGHVFQALQYLRKLCNHPALVLTPQHPEFKTTAEKLAVQNSSLHDI QHAPKLSALKQLLLDCGLGNGSTSESGTESVVAQHRILIFCQLKSMLDIVEHDLLKPHLPSVTYLRLDGS IPPGQRHSIVSRFNNDPSIDVLLLTTHVGGLGLNLTGADTVVFVEHDWNPMRDLQAMDRAHRIGQKRVVN VYRLITRGTLEEKIMGLQKFKMNIANTVISQENSSLQSMGTDQLLDLFTLDKDGKAEKADTSTSGKASMK SILENLSDLWDQEQYDSEYSLENFMHSLK TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003972 |
| ORF Size | 5547 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_003972.3 |
| RefSeq Size | 7054 bp |
| RefSeq ORF | 5550 bp |
| Locus ID | 9044 |
| UniProt ID | O14981 |
| Protein Families | Transcription Factors |
| Gene Summary | This gene encodes a TAF (TATA box-binding protein-associated factor), which associates with TBP (TATA box-binding protein) to form the B-TFIID complex that is required for transcription initiation of genes by RNA polymerase II. This TAF has DNA-dependent ATPase activity, which drives the dissociation of TBP from DNA, freeing the TBP to associate with other TATA boxes or TATA-less promoters. [provided by RefSeq, Sep 2011] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC224324 | BTAF1 (Myc-DDK-tagged)-Human BTAF1 RNA polymerase II, B-TFIID transcription factor-associated, 170kDa (Mot1 homolog, S. cerevisiae) (BTAF1) |
CNY 12,432.00 |
|
| SC315377 | BTAF1 (untagged)-Human BTAF1 RNA polymerase II, B-TFIID transcription factor-associated, 170kDa (Mot1 homolog, S. cerevisiae) (BTAF1) |
CNY 26,790.00 |
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