Aquaporin 9 (AQP9) (NM_020980) Human Tagged ORF Clone

CAT#: RG224567

  • TrueORF®

AQP9 (tGFP-tagged) - Human aquaporin 9 (AQP9)

ORF Plasmid: DDK tGFP



  "NM_020980" in other vectors (6)

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CNY 4,000.00

CNY 4,370.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


AQP9 rabbit polyclonal antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag TurboGFP
Synonyms AQP-9; HsT17287; SSC1; T17287
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RG224567 representing NM_020980
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAGCCTGAGGGAGCAGAAAAGGGAAAAAGCTTCAAGCAGAGACTGGTCTTGAAGAGCAGCTTAGCGA
AAGAAACCCTCTCTGAGTTCTTGGGCACGTTCATCTTGATTGTCCTTGGATGTGGCTGTGTTGCCCAAGC
TATTCTCAGTCGAGGACGTTTTGGAGGGGTCATCACTATCAATGTTGGATTTTCAATGGCAGTTGCAATG
GCCATTTATGTGGCTGGCGGTGTCTCTGGTGGTCACATCAACCCAGCTGTGTCTTTAGCAATGTGTCTCT
TTGGACGGATGAAATGGTTCAAATTGCCATTTTATGTGGGAGCCCAGTTCTTGGGAGCCTTTGTGGGGGC
TGCAACCGTCTTTGGCATTTACTATGATGGACTTATGTCCTTTGCTGGTGGAAAACTGCTGATCGTGGGA
GAAAATGCAACAGCACACATTTTTGCAACATACCCAGCTCCGTATCTATCTCTGGCGAACGCATTTGCAG
ATCAAGTGGTGGCCACCATGATACTCCTCATAATCGTCTTTGCCATTTTTGACTCCAGAAACTTGGGAGC
CCCCAGAGGCCTAGAGCCCATTGCCATCGGCCTCCTGATTATTGTCATTGCTTCCTCCCTGGGACTGAAC
AGTGGCTGTGCCATGAACCCAGCTCGAGACCTGAGTCCCAGACTTTTCACTGCCTTGGCAGGCTGGGGGT
TTGAAGTCTTCAGAGCTGGAAACAACTTCTGGTGGATTCCTGTAGTGGGCCCTTTGGTTGGTGCTGTCAT
TGGAGGCCTCATCTATGTTCTTGTCATTGAAATCCACCATCCAGAGCCTGACTCAGTCTTTAAGGCAGAA
CAATCTGAGGACAAACCAGAGAAATATGAACTCAGTGTCATCATG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG224567 representing NM_020980
Red=Cloning site Green=Tags(s)

MQPEGAEKGKSFKQRLVLKSSLAKETLSEFLGTFILIVLGCGCVAQAILSRGRFGGVITINVGFSMAVAM
AIYVAGGVSGGHINPAVSLAMCLFGRMKWFKLPFYVGAQFLGAFVGAATVFGIYYDGLMSFAGGKLLIVG
ENATAHIFATYPAPYLSLANAFADQVVATMILLIIVFAIFDSRNLGAPRGLEPIAIGLLIIVIASSLGLN
SGCAMNPARDLSPRLFTALAGWGFEVFRAGNNFWWIPVVGPLVGAVIGGLIYVLVIEIHHPEPDSVFKAE
QSEDKPEKYELSVIM

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_020980
ORF Size 885 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_020980.2, NP_066190.1
RefSeq Size 2948 bp
RefSeq ORF 888 bp
Locus ID 366
UniProt ID O43315
Domains MIP
Protein Families Druggable Genome, Transmembrane
Gene Summary The aquaporins are a family of water-selective membrane channels. This gene encodes a member of a subset of aquaporins called the aquaglyceroporins. This protein allows passage of a broad range of noncharged solutes and also stimulates urea transport and osmotic water permeability. This protein may also facilitate the uptake of glycerol in hepatic tissue . The encoded protein may also play a role in specialized leukocyte functions such as immunological response and bactericidal activity. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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