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RAE1 (NM_003610) Human Tagged ORF Clone
CAT＃: RG224655
- TrueORF®
RAE1 (tGFP-tagged) - Human RAE1 RNA export 1 homolog (S. pombe) (RAE1), transcript variant 1
ORF Plasmid: DDK
"NM_003610" in other vectors (6)
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CNY 5,256.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | dJ481F12.3; dJ800J21.1; Gle2; MIG14; Mnrp41; MRNP41 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG224655 representing NM_003610
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCCTGTTTGGAACAACCTCAGGTTTTGGAACCAGTGGGACCAGCATGTTTGGCAGTGCAACTACAG ACAATCACAATCCCATGAAGGATATTGAAGTAACATCATCTCCTGATGATAGCATTGGTTGTCTGTCTTT TAGCCCACCAACCTTGCCGGGGAACTTTCTTATTGCAGGATCATGGGCTAATGATGTTCGCTGCTGGGAA GTTCAAGACAGTGGACAGACCATTCCAAAAGCCCAGCAGATGCACACTGGGCCTGTGCTTGATGTCTGCT GGAGTGACGATGGGAGCAAAGTGTTTACGGCATCGTGTGATAAAACTGCCAAAATGTGGGACCTCAGCAG TAACCAAGCGATACAGATCGCACAGCATGATGCTCCTGTTAAAACCATCCATTGGATCAAAGCTCCAAAC TACAGCTGTGTGATGACTGGGAGCTGGGATAAGACTTTAAAGTTTTGGGATACTCGATCGTCAAATCCTA TGATGGTTTTGCAACTCCCTGAAAGGTGTTACTGTGCTGACGTGATATACCCCATGGCTGTGGTGGCAAC TGCAGAGAGGGGCCTGATTGTCTATCAGCTAGAGAATCAACCTTCTGAATTCAGGAGGATAGAATCTCCA CTGAAACATCAGCATCGGTGTGTGGCTATTTTTAAAGACAAACAGAACAAGCCTACTGGTTTTGCCCTGG GAAGTATCGAGGGGAGAGTTGCTATTCACTATATCAACCCCCCGAACCCCGCCAAAGATAACTTCACCTT TAAATGTCATCGATCTAATGGAACCAACACTTCAGCTCCTCAGGACATTTATGCGGTAAATGGAATCGCG TTCCATCCTGTTCATGGCACCCTTGCAACTGTGGGATCTGATGGTAGATTCAGCTTCTGGGACAAAGATG CCAGAACAAAACTAAAAACTTCGGAACAGTTAGATCAGCCCATCTCAGCTTGCTGTTTCAATCACAATGG AAACATATTTGCATACGCTTCCAGCTACGACTGGTCAAAGGGACATGAATTTTATAATCCCCAGAAAAAA AATTACATTTTCCTGCGTAATGCAGCCGAAGAGCTAAAGCCCAGGAATAAGAAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG224655 representing NM_003610
Red=Cloning site Green=Tags(s) MSLFGTTSGFGTSGTSMFGSATTDNHNPMKDIEVTSSPDDSIGCLSFSPPTLPGNFLIAGSWANDVRCWE VQDSGQTIPKAQQMHTGPVLDVCWSDDGSKVFTASCDKTAKMWDLSSNQAIQIAQHDAPVKTIHWIKAPN YSCVMTGSWDKTLKFWDTRSSNPMMVLQLPERCYCADVIYPMAVVATAERGLIVYQLENQPSEFRRIESP LKHQHRCVAIFKDKQNKPTGFALGSIEGRVAIHYINPPNPAKDNFTFKCHRSNGTNTSAPQDIYAVNGIA FHPVHGTLATVGSDGRFSFWDKDARTKLKTSEQLDQPISACCFNHNGNIFAYASSYDWSKGHEFYNPQKK NYIFLRNAAEELKPRNKK TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003610 |
| ORF Size | 1104 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_003610.4 |
| RefSeq Size | 1815 bp |
| RefSeq ORF | 1107 bp |
| Locus ID | 8480 |
| UniProt ID | P78406 |
| Domains | WD40 |
| Gene Summary | Mutations in the Schizosaccharomyces pombe Rae1 and Saccharomyces cerevisiae Gle2 genes have been shown to result in accumulation of poly(A)-containing mRNA in the nucleus, suggesting that the encoded proteins are involved in RNA export. The protein encoded by this gene is a homolog of yeast Rae1. It contains four WD40 motifs, and has been shown to localize to distinct foci in the nucleoplasm, to the nuclear rim, and to meshwork-like structures throughout the cytoplasm. This gene is thought to be involved in nucleocytoplasmic transport, and in directly or indirectly attaching cytoplasmic mRNPs to the cytoskeleton. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC224655 | RAE1 (Myc-DDK-tagged)-Human RAE1 RNA export 1 homolog (S. pombe) (RAE1), transcript variant 1 |
CNY 3,656.00 |
|
| RC224655L1 | Lenti ORF clone of Human RAE1 RNA export 1 homolog (S. pombe) (RAE1), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC224655L2 | Lenti ORF clone of Human RAE1 RNA export 1 homolog (S. pombe) (RAE1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC224655L3 | Lenti ORF clone of Human RAE1 RNA export 1 homolog (S. pombe) (RAE1), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC224655L4 | Lenti ORF clone of Human RAE1 RNA export 1 homolog (S. pombe) (RAE1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| SC117882 | RAE1 (untagged)-Human RAE1 RNA export 1 homolog (S. pombe) (RAE1), transcript variant 1 |
CNY 3,656.00 |
