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Apc6 (CDC16) (NM_001078645) Human Tagged ORF Clone
CAT＃: RG224869
- TrueORF®
CDC16 (tGFP-tagged) - Human cell division cycle 16 homolog (S. cerevisiae) (CDC16), transcript variant 2
ORF Plasmid: DDK
"NM_001078645" in other vectors (4)
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CNY 6,216.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | ANAPC6; APC6; CDC16Hs; CUT9 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG224869 representing NM_001078645
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACCTAGAGCGGCTGCGGAAGCGCGTCCGGCAGTACCTCGACCAGCAACAGTATCAAAGTGCTCTAT TTTGGGCAGATAAAGTAGCTTCACTCTCTCGTGAAGAACCCCAGGACATCTATTGGTTGGCTCAGTGTCT TTACCTGACAGCACAATATCACAGAGCCGCCCATGCACTTCGGTCACGAAAACTGGACAAATTGTATGAA GCATGTCGTTACCTTGCAGCTAGGTGCCATTATGCTGCAAAAGAGCACCAGCAGGCCCTTGATGTTCTTG ACATGGAAGAGCCCATCAATAAAAGATTATTTGAAAAATACTTGAAGGACGAAAGTGGCTTCAAAGATCC TTCCAGCGACTGGGAAATGTCACAGTCTTCAATAAAGAGTTCTATCTGTCTTCTACGCGGGAAAATCTAT GATGCTCTAGATAACCGAACCCTGGCTACCTACAGCTACAAAGAAGCTTTGAAGCTTGATGTCTACTGTT TTGAAGCGTTCGATCTTTTAACATCACATCACATGCTGACAGCACAAGAAGAAAAAGAACTTCTTGAATC ACTACCCCTTAGCAAGCTGTGTAATGAAGAACAGGAATTGCTGCGTTTTCTATTTGAGAACAAATTGAAA AAATATAATAAGCCTAGTGAAACGGTCATCCCTGAATCTGTAGATGGCTTGCAAGAGAATCTGGATGTGG TAGTGTCTTTAGCTGAGAGACATTATTATAACTGTGATTTTAAAATGTGCTACAAGCTTACTTCTGTAGT AATGGAGAAAGATCCTTTCCATGCAAGTTGTTTACCTGTACATATAGGGACGCTTGTAGAGCTGAATAAA GCCAATGAACTTTTCTATCTTTCTCATAAACTGGTGGATTTATATCCTAGTAATCCTGTGTCTTGGTTTG CAGTGGGATGTTACTATCTCATGGTCGGTCATAAAAATGAACATGCCAGAAGATATCTCAGCAAAGCCAC AACACTTGAGAAAACCTATGGACCTGCATGGATAGCCTATGGACATTCATTTGCGGTGGAGAGTGAGCAC GACCAAGCGATGGCTGCTTACTTCACAGCAGCACAGCTGATGAAAGGGTGTCATTTGCCTATGCTGTATA TTGGATTAGAATATGGTTTGACCAATAACTCAAAACTAGCTGAAAGGTTCTTCAGCCAAGCTCTGAGCAT TGCACCGGAAGACCCTTTTGTTATGCATGAGGTCGGCGTGGTTGCATTTCAGAATGGAGAATGGAAAACA GCCGAAAAATGGTTTCTTGATGCTTTGGAAAAAATTAAAGCAATTGGGAACGAGGTAACAGTTGACAAAT GGGAACCTTTGTTGAACAACTTGGGGCATGTCTGCAGAAAACTTAAAAAGTATGCTGAGGCCTTGGATTA CCACCGTCAGGCACTGGTGTTGATTCCTCAGAACGCATCCACCTACTCTGCTATTGGATATATCCACAGT CTGATGGGCAACTTTGAAAATGCTGTGGACTACTTCCACACAGCCCTTGGTCTTAGGCGAGATGATACAT TTTCTGTTACAATGCTTGGTCATTGCATCGAAATGTACATTGGTGATTCTGAAGCTTATATTGGAGCAGA CATTAAAGACAAATTAAAATGTTATGACTTTGATGTGCATACAATGAAGACACTAAAAAACATTATTTCA CCTCCGTGGGATTTCAGGGAATTTGAAGTAGAAAAACAGACTGCAGAAGAAACGGGGCTTACGCCATTGG AAACCTCAAGGAAAACTCCAGATTCCAGACCTTCCTTGGAAGAAACCTTTGAAATTGAAATGAATGAAAG TGACATGATGTTAGAGACATCTATGTCAGACCACAGCACG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG224869 representing NM_001078645
Red=Cloning site Green=Tags(s) MNLERLRKRVRQYLDQQQYQSALFWADKVASLSREEPQDIYWLAQCLYLTAQYHRAAHALRSRKLDKLYE ACRYLAARCHYAAKEHQQALDVLDMEEPINKRLFEKYLKDESGFKDPSSDWEMSQSSIKSSICLLRGKIY DALDNRTLATYSYKEALKLDVYCFEAFDLLTSHHMLTAQEEKELLESLPLSKLCNEEQELLRFLFENKLK KYNKPSETVIPESVDGLQENLDVVVSLAERHYYNCDFKMCYKLTSVVMEKDPFHASCLPVHIGTLVELNK ANELFYLSHKLVDLYPSNPVSWFAVGCYYLMVGHKNEHARRYLSKATTLEKTYGPAWIAYGHSFAVESEH DQAMAAYFTAAQLMKGCHLPMLYIGLEYGLTNNSKLAERFFSQALSIAPEDPFVMHEVGVVAFQNGEWKT AEKWFLDALEKIKAIGNEVTVDKWEPLLNNLGHVCRKLKKYAEALDYHRQALVLIPQNASTYSAIGYIHS LMGNFENAVDYFHTALGLRRDDTFSVTMLGHCIEMYIGDSEAYIGADIKDKLKCYDFDVHTMKTLKNIIS PPWDFREFEVEKQTAEETGLTPLETSRKTPDSRPSLEETFEIEMNESDMMLETSMSDHST TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001078645 |
| ORF Size | 1860 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001078645.3 |
| RefSeq Size | 2317 bp |
| RefSeq ORF | 1863 bp |
| Locus ID | 8881 |
| UniProt ID | Q13042 |
| Protein Families | Druggable Genome |
| Protein Pathways | Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation, Ubiquitin mediated proteolysis |
| Gene Summary | The protein encoded by this gene functions as a protein ubiquitin ligase and is a component of the multiprotein APC complex. The APC complex is a cyclin degradation system that governs exit from mitosis by targeting cell cycle proteins for degredation by the 26S proteasome. Each component protein of the APC complex is highly conserved among eukaryotic organisms. This protein, and other APC complex proteins, contain a tetratricopeptide repeat (TPR) domain; a protein domain that is often involved in protein-protein interactions and the assembly of multiprotein complexes. Multiple alternatively spliced transcript variants, encoding distinct proteins, have been identified. [provided by RefSeq, Jan 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC224869 | CDC16 (Myc-DDK-tagged)-Human cell division cycle 16 homolog (S. cerevisiae) (CDC16), transcript variant 2 |
CNY 4,616.00 |
|
| RC224869L3 | Lenti ORF clone of Human cell division cycle 16 homolog (S. cerevisiae) (CDC16), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC224869L4 | Lenti ORF clone of Human cell division cycle 16 homolog (S. cerevisiae) (CDC16), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| SC315597 | CDC16 (untagged)-Human cell division cycle 16 homolog (S. cerevisiae) (CDC16), transcript variant 2 |
CNY 7,220.00 |
