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Epsin 1 (EPN1) (NM_001130071) Human Tagged ORF Clone
CAT＃: RG226076
- TrueORF®
EPN1 (tGFP-tagged) - Human epsin 1 (EPN1), transcript variant 1
ORF Plasmid: DDK
"NM_001130071" in other vectors (4)
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CNY 5,040.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG226076 representing NM_001130071
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTGATCAGAGCTGGCTCTGGAACCAGGCTGCTCCAGGAGTAAGGAGCCCAGTCTTTGCATGCAGTG TTGAAAAAGGTAATGTCCCTCTTGTGCTGAGCGAGCACCTGGCACACAGCAGGGACCCAGGCAGTGGGGC TGTTAGGTTCCTTATCTCTCCTGAGCCTTGGGCTTCCGCTATCCTTGGGACGTCTGGTCTTCTGGCTTCC CCGGTTCTTCCTGCCGCCCTGGATGCGGTGACCTGCCAGCACCTGCCGCAGCCTTCGTCCGGGAGTCGCC CCATCTCTCCACGCATCGGGGCCCTGTGCCCCTTGCTGCTGCAGCCGGGCACCATGTCGACCTCGTCCTT GAGGCGCCAGATGAAGAACATCGTCCACAACTACTCAGAGGCGGAGATCAAGGTTCGAGAGGCCACGAGC AATGACCCCTGGGGCCCATCCAGCTCCCTCATGTCAGAGATTGCCGACCTCACCTACAACGTTGTCGCCT TCTCGGAGATCATGAGCATGATCTGGAAGCGGCTCAATGACCATGGCAAGAACTGGCGTCACGTTTACAA GGCCATGACGCTGATGGAGTACCTCATCAAGACCGGCTCGGAGCGCGTGTCGCAGCAGTGCAAGGAGAAC ATGTACGCCGTGCAGACGCTGAAGGACTTCCAGTACGTGGACCGCGACGGCAAGGACCAGGGCGTGAACG TGCGTGAGAAAGCTAAGCAGCTGGTGGCCCTGCTGCGCGACGAGGACCGGCTGCGGGAAGAGCGGGCGCA CGCGCTCAAGACCAAGGAAAAGCTGGCACAGACCGCCACGGCCTCATCAGCAGCTGTGGGCTCAGGCCCC CCTCCCGAGGCGGAGCAGGCGTGGCCGCAGAGCAGCGGGGAGGAGGAGCTGCAGCTCCAGCTGGCCCTGG CCATGAGCAAGGAGGAGGCCGACCAGGAGGAGCGGATCCGTCGCGGGGATGACCTGCGGCTGCAGATGGC AATCGAGGAGAGCAAGAGGGAGACTGGGGGCAAGGAGGAGTCGTCCCTCATGGACCTTGCTGACGTCTTC ACGGCCCCAGCTCCTGCCCCGACCACAGACCCCTGGGGGGGCCCAGCACCCATGGCTGCTGCCGTCCCCA CGGCTGCCCCCACCTCGGACCCCTGGGGCGGCCCCCCTGTCCCTCCAGCTGCTGATCCCTGGGGAGGTCC AGCCCCCACGCCGGCCTCTGGGGACCCCTGGAGGCCTGCTGCCCCTGCAGGACCCTCAGTTGACCCTTGG GGTGGGACCCCAGCCCCTGCAGCTGGGGAGGGGCCCACGCCTGATCCATGGGGAAGTTCCGATGGTGGGG TCCCGGTCAGTGGGCCCTCAGCCTCCGATCCCTGGACACCGGCCCCGGCCTTCTCAGATCCCTGGGGAGG GTCACCTGCCAAGCCCAGCACCAATGGCACAACAGCAGCCGGGGGATTCGACACGGAGCCCGACGAGTTC TCTGACTTTGACCGACTCCGCACGGCACTGCCGACCTCCGGGAGCAGCGCAGGAGAGCTGGAGCTGCTGG CAGGAGAGGTGCCGGCCCGAAGCCCTGGGGCGTTTGACATGAGTGGGGTCAGGGGATCTCTGGCTGAGGC TGTGGGCAGCCCCCCACCTGCAGCCACACCAACTCCCACGCCCCCCACCCGGAAGACGCCGGAGTCATTC CTGGGGCCCAATGCAGCCCTCGTCGACCTGGACTCGCTGGTGAGCCGGCCGGGCCCCACGCCGCCTGGAG CCAAGGCCTCCAACCCCTTCCTGCCAGGCGGAGGCCCAGCCACTGGCCCTTCCGTCACCAACCCCTTCCA GCCCGCGCCTCCCGCGACGCTCACCCTGAACCAGCTCCGTCTCAGTCCTGTGCCTCCCGTCCCTGGAGCG CCACCCACGTACATCTCTCCCCTTGGCGGGGGCCCTGGCCTGCCCCCCATGATGCCCCCGGGCCCCCCGG CCCCCAACACTAATCCCTTCCTCCTA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG226076 representing NM_001130071
Red=Cloning site Green=Tags(s) MGDQSWLWNQAAPGVRSPVFACSVEKGNVPLVLSEHLAHSRDPGSGAVRFLISPEPWASAILGTSGLLAS PVLPAALDAVTCQHLPQPSSGSRPISPRIGALCPLLLQPGTMSTSSLRRQMKNIVHNYSEAEIKVREATS NDPWGPSSSLMSEIADLTYNVVAFSEIMSMIWKRLNDHGKNWRHVYKAMTLMEYLIKTGSERVSQQCKEN MYAVQTLKDFQYVDRDGKDQGVNVREKAKQLVALLRDEDRLREERAHALKTKEKLAQTATASSAAVGSGP PPEAEQAWPQSSGEEELQLQLALAMSKEEADQEERIRRGDDLRLQMAIEESKRETGGKEESSLMDLADVF TAPAPAPTTDPWGGPAPMAAAVPTAAPTSDPWGGPPVPPAADPWGGPAPTPASGDPWRPAAPAGPSVDPW GGTPAPAAGEGPTPDPWGSSDGGVPVSGPSASDPWTPAPAFSDPWGGSPAKPSTNGTTAAGGFDTEPDEF SDFDRLRTALPTSGSSAGELELLAGEVPARSPGAFDMSGVRGSLAEAVGSPPPAATPTPTPPTRKTPESF LGPNAALVDLDSLVSRPGPTPPGAKASNPFLPGGGPATGPSVTNPFQPAPPATLTLNQLRLSPVPPVPGA PPTYISPLGGGPGLPPMMPPGPPAPNTNPFLL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001130071 |
| ORF Size | 1986 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001130071.1, NP_001123543.1 |
| RefSeq Size | 2964 bp |
| RefSeq ORF | 1989 bp |
| Locus ID | 29924 |
| UniProt ID | Q9Y6I3 |
| Protein Pathways | Endocytosis |
| Gene Summary | This gene encodes a member of the epsin protein family. The encoded protein binds clathrin and is involved in the endocytosis of clathrin-coated vesicles. Loss of function of this gene is associated with reduced tumor growth and progression in certain cancer types. [provided by RefSeq, Mar 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC226076 | EPN1 (Myc-DDK-tagged)-Human epsin 1 (EPN1), transcript variant 1 |
CNY 4,928.00 |
|
| RC226076L3 | Lenti-ORF clone of EPN1 (Myc-DDK-tagged)-Human epsin 1 (EPN1), transcript variant 1 |
CNY 6,460.00 |
|
| RC226076L4 | Lenti-ORF clone of EPN1 (mGFP-tagged)-Human epsin 1 (EPN1), transcript variant 1 |
CNY 6,460.00 |
|
| SC326155 | EPN1 (untagged)-Human epsin 1 (EPN1), transcript variant 1 |
CNY 10,550.00 |
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