CD299 (CLEC4M) (NM_001144904) Human Tagged ORF Clone
CAT#: RG226638
- TrueORF®
CLEC4M (tGFP-tagged) - Human C-type lectin domain family 4, member M (CLEC4M), transcript variant 2. Note: ORF is codon optimized
ORF Plasmid: DDK
"NM_001144904" in other vectors (2)
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CNY 4,370.00
CNY 1,000.00
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | CD209L; CD299; DC-SIGN2; DC-SIGNR; DCSIGNR; HP10347; L-SIGN; LSIGN |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG226638 ORF sequence, codon optimized.
Due to the complexity of NM_001144904, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCGATTCAAAAGAACCCCGGGTGCAGCAACTGGGGCTGCTCGGGTGCCTCGGGCATGGCGCATTGG TCCTGCAACTCCTGAGTTTTATGTTGCTGGCTGGTGTGCTGGTGGCTATCCTCGTACAGGTAAGCAAAGT CCCGTCTTCTCTCTCCCAGGAGCAGTCAGAGCAGGATGCTATCTACCAAAATTTGACCCAGCTGAAGGCG GCCGTCGGAGAGCTTAGCGAGAAGTCTAAACTTCAGGAGATTTATCAAGAACTGACCCAACTCAAAGCCG CCGTGGGAGAGCTGCCCGAGAAAAGCAAGCTCCAAGAAATTTACCAGGAACTGACGAGGCTTAAGGCTGC AGTCGGCGAGCTGCCTGAAAAGAGCAAGCTGCAGGAGATCTATCAAGAGTTGACAAGGCTGAAGGCGGCT GTAGGGGAGCTTCCCGAAAAGTCTAAGCTCCAGGAGATCTATCAGGAACTCACTGAACTCAAAGCCGCTG TAGGGGAACTCCCCGAGAAAAGTAAGCTTCAGGAAATCTACCAAGAACTCACACAGCTGAAAGCAGCAGT TGGCGAACTGCCGGACCAGTCCAAACAGCAGCAGATCTACCAGGAATTGACTGACCTCAAAACAGCCTTC GAACGCCTGTGCCGGCACTGCCCCAAAGATTGGACTTTCTTCCAAGGCAACTGCTATTTTATGTCTAATA GCCAGCGCAACTGGCACGACTCCGTGACCGCATGTCAGGAAGTGAGGGCGCAGCTCGTGGTGATCAAAAC CGCCGAGGAACAGAACTTCCTCCAACTGCAAACTTCAAGAAGCAATCGCTTCAGCTGGATGGGGCTGTCC GATTTGAATCAGGAGGGGACCTGGCAGTGGGTCGACGGCTCTCCGCTGTCTCCATCTTTCCAGCGCTATT GGAACTCTGGAGAACCCAACAACTCCGGCAATGAGGACTGTGCTGAATTTAGTGGGAGTGGTTGGAATGA TAATAGGTGTGACGTGGATAACTACTGGATTTGCAAAAAACCGGCAGCCTGCTTCAGGGACGAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG226638 representing NM_001144904
Red=Cloning site Green=Tags(s) MSDSKEPRVQQLGLLGCLGHGALVLQLLSFMLLAGVLVAILVQVSKVPSSLSQEQSEQDAIYQNLTQLKA AVGELSEKSKLQEIYQELTQLKAAVGELPEKSKLQEIYQELTRLKAAVGELPEKSKLQEIYQELTRLKAA VGELPEKSKLQEIYQELTELKAAVGELPEKSKLQEIYQELTQLKAAVGELPDQSKQQQIYQELTDLKTAF ERLCRHCPKDWTFFQGNCYFMSNSQRNWHDSVTACQEVRAQLVVIKTAEEQNFLQLQTSRSNRFSWMGLS DLNQEGTWQWVDGSPLSPSFQRYWNSGEPNNSGNEDCAEFSGSGWNDNRCDVDNYWICKKPAACFRDE TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001144904 |
ORF Size | 1044 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001144904.1, NP_001138376.1 |
RefSeq Size | 1799 bp |
RefSeq ORF | 1047 bp |
Locus ID | 10332 |
Protein Families | Druggable Genome, Transmembrane |
Gene Summary | This gene encodes a C-type lectin that functions in cell adhesion and pathogen recognition. This receptor recognizes a wide range of evolutionarily divergent pathogens with a large impact on public health, including tuberculosis mycobacteria, and viruses including Ebola, hepatitis C, HIV-1, influenza A, West Nile virus and the SARS-CoV acute respiratory syndrome coronavirus. The protein is organized into four distinct domains: a C-terminal carbohydrate recognition domain, a flexible tandem-repeat neck domain of variable length, a transmembrane region and an N-terminal cytoplasmic domain involved in internalization. This gene is closely related in terms of both sequence and function to a neighboring gene, CD209 (Gene ID: 30835), also known as DC-SIGN. The two genes differ in viral recognition and expression patterns, with this gene showing high expression in endothelial cells of the liver, lymph node and placenta. Polymorphisms in the tandem repeat neck domain are associated with resistance to SARS infection. [provided by RefSeq, May 2020] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC226638 | CLEC4M (Myc-DDK-tagged)-Human C-type lectin domain family 4, member M (CLEC4M), transcript variant 2. Note: ORF is codon optimized |
CNY 3,656.00 |
|
SC325801 | CLEC4M (untagged)-Human C-type lectin domain family 4, member M (CLEC4M), transcript variant 2 |
CNY 5,610.00 |