GDNF Receptor alpha 1 (GFRA1) (NM_001145453) Human Tagged ORF Clone
CAT#: RG226704
- TrueORF®
GFRA1 (tGFP-tagged) - Human GDNF family receptor alpha 1 (GFRA1), transcript variant 3
ORF Plasmid: DDK
"NM_001145453" in other vectors (4)
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CNY 4,370.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | GDNFR; GDNFRA; GFR-ALPHA-1; GFRalpha-1; RET1L; RETL1; TRNR1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG226704 representing NM_001145453
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCCTGGCGACCCTGTACTTCGCGCTGCCGCTCTTGGACTTGCTCCTGTCGGCCGAAGTGAGCGGCG GAGACCGCCTGGATTGCGTGAAAGCCAGTGATCAGTGCCTGAAGGAGCAGAGCTGCAGCACCAAGTACCG CACGCTAAGGCAGTGCGTGGCGGGCAAGGAGACCAACTTCAGCCTGGCATCCGGCCTGGAGGCCAAGGAT GAGTGCCGCAGCGCCATGGAGGCCCTGAAGCAGAAGTCGCTCTACAACTGCCGCTGCAAGCGGGGTATGA AGAAGGAGAAGAACTGCCTGCGCATTTACTGGAGCATGTACCAGAGCCTGCAGGGAAATGATCTGCTGGA GGATTCCCCATATGAACCAGTTAACAGCAGATTGTCAGATATATTCCGGGTGGTCCCATTCATATCAGTG GAGCACATTCCCAAAGGGAACAACTGCCTGGATGCAGCGAAGGCCTGCAACCTCGACGACATTTGCAAGA AGTACAGGTCGGCGTACATCACCCCGTGCACCACCAGCGTGTCCAATGATGTCTGCAACCGCCGCAAGTG CCACAAGGCCCTCCGGCAGTTCTTTGACAAGGTCCCGGCCAAGCACAGCTACGGAATGCTCTTCTGCTCC TGCCGGGACATCGCCTGCACAGAGCGGAGGCGACAGACCATCGTGCCTGTGTGCTCCTATGAAGAGAGGG AGAAGCCCAACTGTTTGAATTTGCAGGACTCCTGCAAGACGAATTACATCTGCAGATCTCGCCTTGCGGA TTTTTTTACCAACTGCCAGCCAGAGTCAAGGTCTGTCAGCAGCTGTCTAAAGGAAAACTACGCTGACTGC CTCCTCGCCTACTCGGGGCTTATTGGCACAGTCATGACCCCCAACTACATAGACTCCAGTAGCCTCAGTG TGGCCCCATGGTGTGACTGCAGCAACAGTGGGAACGACCTAGAAGAGTGCTTGAAATTTTTGAATTTCTT CAAGGACAATACATGTCTTAAAAATGCAATTCAAGCCTTTGGCAATGGCTCCGATGTGACCGTGTGGCAG CCAGCCTTCCCAGTACAGACCACCACTGCCACTACCACCACTGCCCTCCGGGTTAAGAACAAGCCCCTGG GGCCAGCAGGGTCTGAGAATGAAATTCCCACTCATGTTTTGCCACCGTGTGCAAATTTACAGGCACAGAA GCTGAAATCCAATGTGTCGGGCAATACACACCTCTGTATTTCCAATGGTAATTATGAAAAAGAAGGTCTC GGTGCTTCCAGCCACATAACCACAAAATCAATGGCTGCTCCTCCAAGCTGTGGTCTGAGCCCACTGCTGG TCCTGGTGGTAACCGCTCTGTCCACCCTATTATCTTTAACAGAAACATCA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG226704 representing NM_001145453
Red=Cloning site Green=Tags(s) MFLATLYFALPLLDLLLSAEVSGGDRLDCVKASDQCLKEQSCSTKYRTLRQCVAGKETNFSLASGLEAKD ECRSAMEALKQKSLYNCRCKRGMKKEKNCLRIYWSMYQSLQGNDLLEDSPYEPVNSRLSDIFRVVPFISV EHIPKGNNCLDAAKACNLDDICKKYRSAYITPCTTSVSNDVCNRRKCHKALRQFFDKVPAKHSYGMLFCS CRDIACTERRRQTIVPVCSYEEREKPNCLNLQDSCKTNYICRSRLADFFTNCQPESRSVSSCLKENYADC LLAYSGLIGTVMTPNYIDSSSLSVAPWCDCSNSGNDLEECLKFLNFFKDNTCLKNAIQAFGNGSDVTVWQ PAFPVQTTTATTTTALRVKNKPLGPAGSENEIPTHVLPPCANLQAQKLKSNVSGNTHLCISNGNYEKEGL GASSHITTKSMAAPPSCGLSPLLVLVVTALSTLLSLTETS TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001145453 |
ORF Size | 1380 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001145453.1, NP_001138925.1 |
RefSeq Size | 9235 bp |
RefSeq ORF | 1383 bp |
Locus ID | 2674 |
UniProt ID | P56159 |
Protein Families | Druggable Genome |
Gene Summary | This gene encodes a member of the glial cell line-derived neurotrophic factor receptor (GDNFR) family of proteins. The encoded preproprotein is proteolytically processed to generate the mature receptor. Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. This receptor is a glycosylphosphatidylinositol (GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. This gene is a candidate gene for Hirschsprung disease. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed. [provided by RefSeq, Jan 2016] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC226704 | GFRA1 (Myc-DDK-tagged)-Human GDNF family receptor alpha 1 (GFRA1), transcript variant 3 |
CNY 3,656.00 |
|
RC226704L3 | Lenti ORF clone of Human GDNF family receptor alpha 1 (GFRA1), transcript variant 3, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC226704L4 | Lenti ORF clone of Human GDNF family receptor alpha 1 (GFRA1), transcript variant 3, mGFP tagged |
CNY 5,890.00 |
|
SC326479 | GFRA1 (untagged)-Human GDNF family receptor alpha 1 (GFRA1), transcript variant 3, mRNA |
CNY 7,220.00 |
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