SPART (NM_001142294) Human Tagged ORF Clone
CAT#: RG227155
- TrueORF®
SPG20 (tGFP-tagged) - Human spastic paraplegia 20 (Troyer syndrome) (SPG20), transcript variant 4
ORF Plasmid: DDK
"NM_001142294" in other vectors (4)
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CNY 4,370.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | SPG20; TAHCCP1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG227155 representing NM_001142294
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCAAGAGCCACAAAATGGAGAACCTGCTGAAATTAAGATCATCAGAGAAGCATATAAGAAGGCCT TTTTATTTGTTAACAAAGGTCTGAATACAGATGAATTAGGTCAGAAGGAAGAAGCAAAGAACTACTATAA GCAAGGAATAGGACACCTGCTCAGAGGGATCAGCATTTCATCAAAAGAGTCTGAACACACAGGTACTGGG TGGGAATCTGCTAGACAGATGCAACAGAAAATGAAAGAAACTCTACAGAATGTACGCACCAGGCTGGAAA TTCTAGAGAAGGGTCTTGCCACTTCTCTGCAGAATGATCTTCAGGAGGTGCCCAAGTTATATCCAGAATT TCCACCTAAAGACATGTGTGAAAAATTACCAGAGCCTCAGTCTTTTAGTTCAGCTCCTCAGCATGCTGAA GTAAATGGAAACACCTCAACTCCAAGTGCAGGGGCAGTTGCTGCACCTGCTTCTCTGTCTTTACCATCAC AAAGTTGTCCAGCAGAAGCTCCTCCTGCTTATACTCCTCAAGCTGCTGAAGGTCACTACACTGTATCCTA TGGAACAGATTCTGGGGAGTTTTCATCAGTTGGAGAGGAGTTTTATAGGAATCATTCTCAGCCACCGCCT CTTGAGACCTTAGGGCTGGATGCAGATGAATTGATTTTGATACCAAATGGAGTACAGATTTTTTTTGTAA ATCCTGCAGGGGAGGTTAGTGCACCTTCGTATCCTGGGTACCTTCGAATTGTGAGGTTTTTGGATAATTC TCTCGATACGGTTCTAAACCGTCCTCCCGGGTTTCTTCAGGTTTGTGACTGGTTATATCCTCTAGTTCCT GATAGATCTCCGGTTCTGAAATGTACTGCGGGAGCCTACATGTTTCCTGATACAATGCTACAAGCAGCAG GATGCTTTGTGGGGGTCGTCCTGTCCTCTGAGTTACCAGAGGATGATAGAGAGCTCTTTGAGGATCTGTT AAGGCAAATGTCTGACCTTCGGCTCCAGGCCAACTGGAACAGAGCAGAAGAAGAAAATGAATTCCAAATC CCTGGAAGAACTAGACCCTCCTCTGACCAACTAAAAGAAGCCTCTGGCACTGATGTGAAACAGTTGGACC AAGGCAATAAGGATGTACGTCATAAAGGAAAACGTGGAAAAAGGGCTAAAGATACTTCAAGTGAAGAAGT TAACCTGAGTCACATTGTACCATGTGAGCCAGTTCCAGAAGAAAAGCCAAAAGAATTACATGAATGGAGT GAAAAAGTGGCTCACAACATTTTGTCAGGTGCTTCCTGGGTGAGTTGGGGTTTAGTCAAAGGTGCTGAGA TTACTGGTAAGGCAATCCAGAAAGGTGCTTCTAAACTCCGAGAGCGGATTCAACCAGAAGAAAAACCCGT GGAAGTTAGTCCAGCTGTCACCAAGGGACTTTATATAGCGAAGCAAGCTACAGGAGGAGCAGCAAAAGTC AGTCAGTTCCTGGTTGATGGAGTTTGCACTGTAGCAAATTGCGTTGGAAAAGAACTAGCTCCACATGTCA AGAAGCATGGAAGCAAACTTGTTCCAGAATCTCTTAAAAAAGACAAAGATGGGAAATCTCCTCTGGATGG TGCTATGGTTGTAGCAGCGAGTAGTGTTCAAGGATTTTCAACTGTCTGGCAAGGATTGGAATGTGCAGCT AAATGCATCGTTAACAATGTTTCAGCAGAAACTGTACAAACTGTCAGATACAAATACGGATATAATGCAG GAGAAGCTACCCACCATGCGGTGGATTCTGCGGTCAATGTTGGCGTAACTGCCTACAATATTAACAACAT TGGTATCAAAGCAATGGTGAAGAAAACTGCAACACAAACAGGACACACTCTCCTTGAGGACTATCAGATA GTTGATAATTCTCAGAGGGAAAATCAAGAAGGAGCAGCAAATGTCAACGTGAGAGGGGAGAAGGATGAGC AGACGAAGGAAGTAAAGGAGGCAAAGAAGAAAGATAAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG227155 representing NM_001142294
Red=Cloning site Green=Tags(s) MEQEPQNGEPAEIKIIREAYKKAFLFVNKGLNTDELGQKEEAKNYYKQGIGHLLRGISISSKESEHTGTG WESARQMQQKMKETLQNVRTRLEILEKGLATSLQNDLQEVPKLYPEFPPKDMCEKLPEPQSFSSAPQHAE VNGNTSTPSAGAVAAPASLSLPSQSCPAEAPPAYTPQAAEGHYTVSYGTDSGEFSSVGEEFYRNHSQPPP LETLGLDADELILIPNGVQIFFVNPAGEVSAPSYPGYLRIVRFLDNSLDTVLNRPPGFLQVCDWLYPLVP DRSPVLKCTAGAYMFPDTMLQAAGCFVGVVLSSELPEDDRELFEDLLRQMSDLRLQANWNRAEEENEFQI PGRTRPSSDQLKEASGTDVKQLDQGNKDVRHKGKRGKRAKDTSSEEVNLSHIVPCEPVPEEKPKELHEWS EKVAHNILSGASWVSWGLVKGAEITGKAIQKGASKLRERIQPEEKPVEVSPAVTKGLYIAKQATGGAAKV SQFLVDGVCTVANCVGKELAPHVKKHGSKLVPESLKKDKDGKSPLDGAMVVAASSVQGFSTVWQGLECAA KCIVNNVSAETVQTVRYKYGYNAGEATHHAVDSAVNVGVTAYNINNIGIKAMVKKTATQTGHTLLEDYQI VDNSQRENQEGAANVNVRGEKDEQTKEVKEAKKKDK TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001142294 |
ORF Size | 1998 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001142294.1, NP_001135766.1 |
RefSeq Size | 4838 bp |
RefSeq ORF | 2001 bp |
Locus ID | 23111 |
UniProt ID | Q8N0X7 |
Gene Summary | This gene encodes a protein containing a MIT (Microtubule Interacting and Trafficking molecule) domain, and is implicated in regulating endosomal trafficking and mitochondria function. The protein localizes to mitochondria and partially co-localizes with microtubules. Stimulation with epidermal growth factor (EGF) results in protein translocation to the plasma membrane, and the protein functions in the degradation and intracellular trafficking of EGF receptor. Multiple alternatively spliced variants, encoding the same protein, have been identified. Mutations associated with this gene cause autosomal recessive spastic paraplegia 20 (Troyer syndrome). [provided by RefSeq, Nov 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC227155 | SPG20 (Myc-DDK-tagged)-Human spastic paraplegia 20 (Troyer syndrome) (SPG20), transcript variant 4 |
CNY 4,960.00 |
|
RC227155L3 | Lenti-ORF clone of SPG20 (Myc-DDK-tagged)-Human spastic paraplegia 20 (Troyer syndrome) (SPG20), transcript variant 4 |
CNY 5,890.00 |
|
RC227155L4 | Lenti-ORF clone of SPG20 (mGFP-tagged)-Human spastic paraplegia 20 (Troyer syndrome) (SPG20), transcript variant 4 |
CNY 5,890.00 |
|
SC325265 | SPG20 (untagged)-Human spastic paraplegia 20 (Troyer syndrome) (SPG20), transcript variant 4 |
CNY 4,888.00 |