CDC20B (NM_001145734) Human Tagged ORF Clone
CAT#: RG227528
- TrueORF®
CDC20B (tGFP-tagged) - Human cell division cycle 20 homolog B (S. cerevisiae) (CDC20B), transcript variant 2
ORF Plasmid: DDK
"NM_001145734" in other vectors (4)
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CNY 4,370.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | G6VTS76519 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG227528 representing NM_001145734
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTGGAAACTGGAGCGCACCGCGCCTCGGAGGGTCCGCACGGAAGAGGAGATGCTGTGGGAAAGTA TCATGCGTGTGCTCTCCAAAGACTTGAAGCAGAAGAGAAGTCAAGATTCCGCCAACGTACTCGATTCAGT TAATGCTACGTATTCTGACTTTAAGAGCAACTTTGCGAAGAGGCTGTCCGCAGAGGTTCCTGTTGCGAGT AGCCCCATTACCACAAGGTGGCAGCAAAGTCAAACTAGGGCTCTGTCCTCTGATTCCTTTGGGGAAGAGC AGTCAACCACCTACCTCCCAGAAGCTTCCGGATCAGTGCTGAAGACACCGCCTGAGAAAGAGACCTTGAC TCTAGGATCCCGCAAAGAACAACTGAAGACCCCCAGCAAAGGAATTTCTGAAACAAGTAACTCTGCTCTC CATTTTTGCAAGGCACCTCATGCAATGGACAGAGACTGGAAAGAAAGTGTTGCCTCAAAAGGGCAGAAAT GCCTAAAACAACTCTTTGTAACCCAGAACGTGGTTCAGCAGGCTAATGGAAAAATGCAGCTCTGTGAGCA ATCCGAATGTGTTTGGAAAGGCTGCAAAGATGGAGTCAGAGATGAATCCTTCCATCTTAAAAGTTCTGGT GATATAAACGATTCCATACTCCAACCAGAGGTGAAGATTCATATTACTGGTCTTCGAAATGACTACTATC TGAATATCCTAGATTGGAGTTTTCAGAATCTTGTTGCCATAGCCCTGGGCTCTGCTGTATACATCTGGAA TGGGGAGAACCACAATGGGATTGAAAACATAGACTTAAGTCTCACTTGTAACTATATCTCTTCTGTGTCC TGGATAAAAGAGGGAACTTGCCTGGCAGTTGGCACCAGCGAGGGAGAAGTGCAATTATGGGATGTGGTAA CTAAAAAGCGGCTGAGAAATATGCTTGGTCATTTGTCAGTAGTTGGGGCTCTGAGCTGGAATCACTTTAT CCTCAGCAGTGGGTCAAGACTGGGGCGTGTTTATCATCACGATGTTCGGGTAGCCCAGCATCATGTTGGA ACACTTCGCCACAAGCAAGCTGTGTGTGCTCTGAAGTGGTCACCGGATGGCAGGCTGCTTTCCAGCGGCT GCAGTGATGGACTGCTGACAATATGGCCCCACGATCCAGGTGCCAGTGCACAGGGCCAACCGCTGAAAGT CATAACCCAGTCTACGGCAGTCAAGATTTGTTCCTTAATCTGGCTACCTAAGACAAAGGAGATTGCAACT GGTCAAGGTACTCCCAAGAATGATGTGACTGTGTGGACCTGTCCCACTGTGTCCAGGTCAGGTGGGTTTT TTGGCCACAGGGGCAGAGTGCTGCACCTGGCTTTGAGTCCAGACCAGACCCGGGTGTTTTCTGCTGCAGC TGATGGGACGGCCTCTGTATGGAATTGCTAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG227528 representing NM_001145734
Red=Cloning site Green=Tags(s) MEWKLERTAPRRVRTEEEMLWESIMRVLSKDLKQKRSQDSANVLDSVNATYSDFKSNFAKRLSAEVPVAS SPITTRWQQSQTRALSSDSFGEEQSTTYLPEASGSVLKTPPEKETLTLGSRKEQLKTPSKGISETSNSAL HFCKAPHAMDRDWKESVASKGQKCLKQLFVTQNVVQQANGKMQLCEQSECVWKGCKDGVRDESFHLKSSG DINDSILQPEVKIHITGLRNDYYLNILDWSFQNLVAIALGSAVYIWNGENHNGIENIDLSLTCNYISSVS WIKEGTCLAVGTSEGEVQLWDVVTKKRLRNMLGHLSVVGALSWNHFILSSGSRLGRVYHHDVRVAQHHVG TLRHKQAVCALKWSPDGRLLSSGCSDGLLTIWPHDPGASAQGQPLKVITQSTAVKICSLIWLPKTKEIAT GQGTPKNDVTVWTCPTVSRSGGFFGHRGRVLHLALSPDQTRVFSAAADGTASVWNCY TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001145734 |
ORF Size | 1431 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001145734.1, NP_001139206.1 |
RefSeq Size | 2832 bp |
RefSeq ORF | 1434 bp |
Locus ID | 166979 |
UniProt ID | Q86Y33 |
Protein Families | Druggable Genome |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC227528 | CDC20B (Myc-DDK-tagged)-Human cell division cycle 20 homolog B (S. cerevisiae) (CDC20B), transcript variant 2 |
CNY 3,656.00 |
|
RC227528L3 | Lenti ORF clone of Human cell division cycle 20 homolog B (S. cerevisiae) (CDC20B), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC227528L4 | Lenti ORF clone of Human cell division cycle 20 homolog B (S. cerevisiae) (CDC20B), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
SC326580 | CDC20B (untagged)-Human cell division cycle 20 homolog B (S. cerevisiae) (CDC20B), transcript variant 2, mRNA |
CNY 7,700.00 |