ECSIT (NM_001142465) Human Tagged ORF Clone
CAT#: RG227595
- TrueORF®
ECSIT (tGFP-tagged) - Human ECSIT homolog (Drosophila) (ECSIT), nuclear gene encoding mitochondrial protein, transcript variant 3
ORF Plasmid: DDK
"NM_001142465" in other vectors (4)
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CNY 4,370.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | SITPEC |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG227595 representing NM_001142465
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCTGGGTCCAGGCCACCCTACTGGCCCGAGGCCTCTGTAGGGCCTGGGGAGGCACCTGCGGGGCCG CCCTCACAGGAACCTCCATCTCTCAGGTTCCTTTGCCCAAAGACTCAACAGGTGCAGCAGATCCCCCCCA GCCCCACATCGTAGGAATCCAGAGTCCCGATCAGCAGGCCGCCCTGGCCCGCCACAATCCAGCCCGGCCT GTCTTTGTTGAGGGCCCCTTCTCCCTGTGGCTCCGCAACAAGTGTGTGTATTACCACATCCTCAGAGCTG ACTTGCTGCCCCCGGAGGAGAGGGAAGTGGAAGAGACGCCGGAGGAGTGGAACCTCTACTACCCGATGCA GCTGGACCTGGAGTATGTGAGGAGTGGCTGGGACAACTACGAGTTTGACATCAATGAAGTGGAGGAAGGC CCTGTCTTCGCCATGTGCATGGCGGGTGCTCATGACCAGGCGACGATGGCTAAGTGGATCCAGGGCCTGC AGGAGACCAACCCAACCCTGGCCCAGATCCCCGTGGTCTTCCGCCTCGCCGGGTCCACCCGGGAGCTCCA GACATCCTCTGCAGGGCTGGAGGAGCCGCCCCTGCCCGAGGACCACCAGGAAGAAGACGACAACCTGCAG CGACAGCAGCAGGGCCAGAGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG227595 representing NM_001142465
Red=Cloning site Green=Tags(s) MSWVQATLLARGLCRAWGGTCGAALTGTSISQVPLPKDSTGAADPPQPHIVGIQSPDQQAALARHNPARP VFVEGPFSLWLRNKCVYYHILRADLLPPEEREVEETPEEWNLYYPMQLDLEYVRSGWDNYEFDINEVEEG PVFAMCMAGAHDQATMAKWIQGLQETNPTLAQIPVVFRLAGSTRELQTSSAGLEEPPLPEDHQEEDDNLQ RQQQGQS TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001142465 |
ORF Size | 651 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001142465.3 |
RefSeq Size | 1061 bp |
RefSeq ORF | 654 bp |
Locus ID | 51295 |
UniProt ID | Q9BQ95 |
Protein Pathways | MAPK signaling pathway |
Gene Summary | Adapter protein of the Toll-like and IL-1 receptor signaling pathway that is involved in the activation of NF-kappa-B via MAP3K1. Promotes proteolytic activation of MAP3K1. Involved in the BMP signaling pathway. Required for normal embryonic development (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC227595 | ECSIT (Myc-DDK-tagged)-Human ECSIT homolog (Drosophila) (ECSIT), nuclear gene encoding mitochondrial protein, transcript variant 3 |
CNY 2,400.00 |
|
RC227595L3 | Lenti ORF clone of Human ECSIT homolog (Drosophila) (ECSIT), nuclear gene encoding mitochondrial protein, transcript variant 3, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC227595L4 | Lenti ORF clone of Human ECSIT homolog (Drosophila) (ECSIT), nuclear gene encoding mitochondrial protein, transcript variant 3, mGFP tagged |
CNY 5,890.00 |
|
SC325617 | ECSIT (untagged)-Human ECSIT homolog (Drosophila) (ECSIT), nuclear gene encoding mitochondrial protein, transcript variant 3 |
CNY 3,990.00 |