BDKRB1 (NM_000710) Human Tagged ORF Clone
CAT#: RG228017
- TrueORF®
BDKRB1 (tGFP-tagged) - Human bradykinin receptor B1 (BDKRB1)
ORF Plasmid: DDK
"NM_000710" in other vectors (9)
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CNY 4,370.00
CNY 1,000.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | B1BKR; B1R; BKB1R; BKR1; BRADYB1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG228017 representing NM_000710
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCATCATCCTGGCCCCCTCTAGAGCTCCAATCCTCCAACCAGAGCCAGCTCTTCCCTCAAAATGCTA CGGCCTGTGACAATGCTCCAGAAGCCTGGGACCTGCTGCACAGAGTGCTGCCAACATTTATCATCTCCAT CTGTTTCTTCGGCCTCCTAGGGAACCTTTTTGTCCTGTTGGTCTTCCTCCTGCCCCGGCGGCAACTGAAC GTGGCAGAAATCTACCTGGCCAACCTGGCAGCCTCTGATCTGGTGTTTGTCTTGGGCTTGCCCTTCTGGG CAGAGAATATCTGGAACCAGTTTAACTGGCCTTTCGGAGCCCTCCTCTGCCGTGTCATCAACGGGGTCAT CAAGGCCAATTTGTTCATCAGCATCTTCCTGGTGGTGGCCATCAGCCAGGACCGCTACCGCGTGCTGGTG CACCCTATGGCCAGCCGGAGGCAGCAGCGGCGGAGGCAGGCCCGGGTCACCTGCGTGCTCATCTGGGTTG TGGGGGGCCTCTTGAGCATCCCCACATTCCTGCTGCGATCCATCCAAGCCGTCCCAGATCTGAACATCAC CGCCTGCATCCTGCTCCTCCCCCATGAGGCCTGGCACTTTGCAAGGATTGTGGAGTTAAATATTCTGGGT TTCCTCCTACCACTGGCTGCGATCGTCTTCTTCAACTACCACATCCTGGCCTCCCTGCGAACGCGGGAGG AGGTCAGCAGGACAAGGTGCGGGGGCCGCAAGGATAGCAAGACCACAGCGCTGATCCTCACGCTCGTGGT TGCCTTCCTGGTCTGCTGGGCCCCTTACCACTTCTTTGCCTTCCTGGAATTCTTATTCCAGGTGCAAGCA GTCCGAGGCTGCTTTTGGGAGGACTTCATTGACCTGGGCCTGCAATTGGCCAACTTCTTTGCCTTCACTA ACAGCTCCCTGAATCCAGTAATTTATGTCTTTGTGGGCCGGCTCTTCAGGACCAAGGTCTGGGAACTTTA TAAACAATGCACCCCTAAAAGTCTTGCTCCAATATCTTCATCCCATAGGAAAGAAATCTTCCAACTTTTC TGGCGGAAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG228017 representing NM_000710
Red=Cloning site Green=Tags(s) MASSWPPLELQSSNQSQLFPQNATACDNAPEAWDLLHRVLPTFIISICFFGLLGNLFVLLVFLLPRRQLN VAEIYLANLAASDLVFVLGLPFWAENIWNQFNWPFGALLCRVINGVIKANLFISIFLVVAISQDRYRVLV HPMASRRQQRRRQARVTCVLIWVVGGLLSIPTFLLRSIQAVPDLNITACILLLPHEAWHFARIVELNILG FLLPLAAIVFFNYHILASLRTREEVSRTRCGGRKDSKTTALILTLVVAFLVCWAPYHFFAFLEFLFQVQA VRGCFWEDFIDLGLQLANFFAFTNSSLNPVIYVFVGRLFRTKVWELYKQCTPKSLAPISSSHRKEIFQLF WRN TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_000710 |
ORF Size | 1059 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_000710.4 |
RefSeq Size | 1307 bp |
RefSeq ORF | 1062 bp |
Locus ID | 623 |
UniProt ID | P46663 |
Protein Families | Druggable Genome, GPCR, Transmembrane |
Protein Pathways | Calcium signaling pathway, Complement and coagulation cascades, Neuroactive ligand-receptor interaction, Regulation of actin cytoskeleton |
Gene Summary | Bradykinin, a 9 aa peptide, is generated in pathophysiologic conditions such as inflammation, trauma, burns, shock, and allergy. The protein encoded by this gene belongs to the G-protein coupled receptor 1 family. Two types of G-protein coupled receptors have been found which bind bradykinin and mediate responses to these pathophysiologic conditions. The protein encoded by this gene is one of these receptors and is synthesized de novo following tissue injury. Receptor binding leads to an increase in the cytosolic calcium ion concentration, ultimately resulting in chronic and acute inflammatory responses. [provided by RefSeq, Aug 2020] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC204436 | BDKRB1 (Myc-DDK-tagged)-Human bradykinin receptor B1 (BDKRB1) |
CNY 3,656.00 |
|
RC204436L1 | Lenti ORF clone of Human bradykinin receptor B1 (BDKRB1), Myc-DDK-tagged |
CNY 6,056.00 |
|
RC204436L2 | Lenti ORF clone of Human bradykinin receptor B1 (BDKRB1), mGFP tagged |
CNY 5,890.00 |
|
RC204436L3 | Lenti ORF clone of Human bradykinin receptor B1 (BDKRB1), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC204436L4 | Lenti ORF clone of Human bradykinin receptor B1 (BDKRB1), mGFP tagged |
CNY 6,056.00 |
|
RC228017 | BDKRB1 (Myc-DDK-tagged)-Human bradykinin receptor B1 (BDKRB1) |
CNY 3,656.00 |
|
RC228017L3 | Lenti ORF clone of Human bradykinin receptor B1 (BDKRB1), Myc-DDK-tagged |
CNY 5,890.00 |
|
RG204436 | BDKRB1 (tGFP-tagged) - Human bradykinin receptor B1 (BDKRB1) |
CNY 5,256.00 |
|
SC122572 | BDKRB1 (untagged)-Human bradykinin receptor B1 (BDKRB1) |
CNY 3,656.00 |