TRPT1 (NM_001160389) Human Tagged ORF Clone
CAT#: RG228190
- TrueORF®
TRPT1 (tGFP-tagged) - Human tRNA phosphotransferase 1 (TRPT1), transcript variant 3
ORF Plasmid: DDK
"NM_001160389" in other vectors (4)
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CNY 4,370.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG228190 representing NM_001160389
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACTTCTCTGGAGGAGGGAGGCAGGAAGCAGCAGGGTCCAGGGGTAGAAGGGCTCCCAGACCCCGAG AACAGGACCGAGACGTGCAGCTGTCCAAGGCTCTGTCCTATGCCCTGCGCCATGGGGCCTTGAAGCTGGG GCTTCCCATGGGAGCTGATGGCTTCGTGCCCCTGGGCACCCTCCTGCAGTTGCCCCAGTTCCGCGGCTTC TCTGCTGAAGATGTGCAGCGCGTGGTGGACACCAATAGGAAGCAGCGGTTCGCCCTGCAGCTGGGGGATC CCAGCACTGGCCTTCTCATCCGGGCCAACCAGGGCCATTCCCTGCAGGTCGGGGTACCTAAGTTGGAGCT GATGCCCCTGGAGACACCGCAGGCCCTGCCCCCGATGCTAGTCCATGGTACATTCTGGAAGCACTGGCCA TCCATCCTACTCAAAGGCCTGTCCTGCCAGGGAAGGACGCACATTCACCTGGCCCCAGGACTGCCTGGAG ACCCCGGTATCATCAGTGGCATGCGGTCCCATTGTGAAATAGCTGTGTTCATCGATGGACCCCTGGCTCT GGCAGATGGAATACCCTTCTTCCGCTCTGCCAATGGGGTGATTCTGACTCCAGGGAATACTGATGGCTTC CTCCTTCCCAAGTACTTCAAGGAGGCCCTGCAGCTACGCCCTACCCGAAAGCCCCTTTCCTTGGCTGGTG ATGAAGAGACAGAGTGTCAGAGTAGCCCCAAGCACAGCTCCAGAGAAAGGAGGAGGATCCAACAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG228190 representing NM_001160389
Red=Cloning site Green=Tags(s) MNFSGGGRQEAAGSRGRRAPRPREQDRDVQLSKALSYALRHGALKLGLPMGADGFVPLGTLLQLPQFRGF SAEDVQRVVDTNRKQRFALQLGDPSTGLLIRANQGHSLQVGVPKLELMPLETPQALPPMLVHGTFWKHWP SILLKGLSCQGRTHIHLAPGLPGDPGIISGMRSHCEIAVFIDGPLALADGIPFFRSANGVILTPGNTDGF LLPKYFKEALQLRPTRKPLSLAGDEETECQSSPKHSSRERRRIQQ TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001160389 |
ORF Size | 765 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001160389.1, NP_001153861.1 |
RefSeq Size | 1077 bp |
RefSeq ORF | 768 bp |
Locus ID | 83707 |
UniProt ID | Q86TN4 |
Gene Summary | Catalyzes the last step of tRNA splicing, the transfer of the splice junction 2'-phosphate from ligated tRNA to NAD to produce ADP-ribose 1''-2'' cyclic phosphate.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC228190 | TRPT1 (Myc-DDK-tagged)-Human tRNA phosphotransferase 1 (TRPT1), transcript variant 3 |
CNY 3,990.00 |
|
RC228190L3 | Lenti-ORF clone of TRPT1 (Myc-DDK-tagged)-Human tRNA phosphotransferase 1 (TRPT1), transcript variant 3 |
CNY 5,890.00 |
|
RC228190L4 | Lenti-ORF clone of TRPT1 (mGFP-tagged)-Human tRNA phosphotransferase 1 (TRPT1), transcript variant 3 |
CNY 5,890.00 |
|
SC326825 | TRPT1 (untagged)-Human tRNA phosphotransferase 1 (TRPT1) transcript variant 3 |
CNY 3,990.00 |