RASSF8 (NM_001164747) Human Tagged ORF Clone
CAT#: RG228332
- TrueORF®
RASSF8 (tGFP-tagged) - Human Ras association (RalGDS/AF-6) domain family (N-terminal) member 8 (RASSF8), transcript variant 2
ORF Plasmid: DDK
"NM_001164747" in other vectors (4)
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CNY 4,370.00
CNY 1,000.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | C12orf2; HOJ1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG228332 representing NM_001164747
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAACTTAAAGTATGGGTGGATGGAGTTCAGAGGATTGTTTGTGGAGTCACTGAAGTCACAACTTGCC AGGAGGTTGTCATAGCCTTAGCTCAAGCAATAGGTCGAACTGGAAGGTACACCCTTATAGAGAAATGGAG AGATACTGAAAGACACTTAGCACCTCATGAAAATCCTATCATATCCTTAAACAAATGGGGGCAGTATGCT AGTGATGTGCAGCTCATTCTACGACGAACTGGGCCGTCTCTCAGTGAGCGACCCACTTCAGACAGTGTGG CTCGAATTCCTGAAAGAACTTTATACAGGCAGAGTCTGCCCCCCTTAGCTAAACTGAGGCCTCAGATTGA CAAATCAATCAAAAGGAGGGAACCGAAAAGGAAATCACTGACATTTACAGGAGGTGCCAAAGGATTAATG GACATTTTTGGAAAAGGTAAAGAAACTGAGTTTAAGCAAAAGGTGCTGAATAACTGCAAAACAACAGCAG ATGAGTTGAAGAAGCTAATCCGTCTGCAGACAGAGAAGCTTCAATCCATTGAGAAACAGCTGGAATCTAA TGAAATAGAAATAAGATTTTGGGAGCAAAAGTATAATTCCAACCTTGAAGAGGAAATTGTCCGTCTAGAG CAAAAGATCAAAAGAAACGATGTAGAAATTGAGGAGGAAGAATTCTGGGAAAATGAATTACAGATTGAAC AGGAAAATGAAAAACAGCTGAAGGATCAACTTCAAGAAATAAGACAGAAAATAACAGAATGTGAAAACAA ATTAAAGGACTATTTGGCACAGATCCGGACTATGGAAAGTGGTCTTGAAGCAGAAAAATTGCAACGGGAA GTTCAAGAGGCACAGGTCAATGAGGAAGAGGTTAAAGGAAAGATCGGTAAGGTCAAAGGGGAGATTGACA TTCAAGGCCAGCAGAGTCTGAGGTTGGAAAATGGCATCAAAGCTGTGGAAAGATCTCTTGGACAAGCCAC CAAACGCTTACAGGACAAAGAACAGGAACTGGAGCAGTTGACTAAGGAGTTGCGGCAAGTCAATCTCCAG CAGTTCATCCAGCAGACAGGGACAAAAGTTACCGTTTTGCCAGCGGAGCCCATTGAAATAGAGGCCTCAC ATGCAGACATTGAAAGGGAGGCACCATTCCAGTCTGGGTCCCTGAAGCGACCTGGTTCATCTCGGCAGCT CCCCAGTAATCTCCGCATTCTGCAGAATCCTATCTCATCTGGTTTTAATCCTGAAGGCATATATGTA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG228332 representing NM_001164747
Red=Cloning site Green=Tags(s) MELKVWVDGVQRIVCGVTEVTTCQEVVIALAQAIGRTGRYTLIEKWRDTERHLAPHENPIISLNKWGQYA SDVQLILRRTGPSLSERPTSDSVARIPERTLYRQSLPPLAKLRPQIDKSIKRREPKRKSLTFTGGAKGLM DIFGKGKETEFKQKVLNNCKTTADELKKLIRLQTEKLQSIEKQLESNEIEIRFWEQKYNSNLEEEIVRLE QKIKRNDVEIEEEEFWENELQIEQENEKQLKDQLQEIRQKITECENKLKDYLAQIRTMESGLEAEKLQRE VQEAQVNEEEVKGKIGKVKGEIDIQGQQSLRLENGIKAVERSLGQATKRLQDKEQELEQLTKELRQVNLQ QFIQQTGTKVTVLPAEPIEIEASHADIEREAPFQSGSLKRPGSSRQLPSNLRILQNPISSGFNPEGIYV TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001164747 |
ORF Size | 1257 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_001164747.2 |
RefSeq Size | 5627 bp |
RefSeq ORF | 1260 bp |
Locus ID | 11228 |
UniProt ID | Q8NHQ8 |
Gene Summary | This gene encodes a member of the Ras-assocation domain family (RASSF) of tumor suppressor proteins. This gene is essential for maintaining adherens junction function in epithelial cells and has a role in epithelial cell migration. It is a lung tumor suppressor gene candidate. A chromosomal translocation t(12;22)(p11.2;q13.3) leading to the fusion of this gene and the FBLN1 gene is found in a complex type of synpolydactyly. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2011] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC228332 | RASSF8 (Myc-DDK-tagged)-Human Ras association (RalGDS/AF-6) domain family (N-terminal) member 8 (RASSF8), transcript variant 2 |
CNY 3,656.00 |
|
RC228332L3 | Lenti-ORF clone of RASSF8 (Myc-DDK-tagged)-Human Ras association (RalGDS/AF-6) domain family (N-terminal) member 8 (RASSF8), transcript variant 2 |
CNY 5,890.00 |
|
RC228332L4 | Lenti-ORF clone of RASSF8 (mGFP-tagged)-Human Ras association (RalGDS/AF-6) domain family (N-terminal) member 8 (RASSF8), transcript variant 2 |
CNY 5,890.00 |
|
SC326967 | RASSF8 (untagged)-Human Ras association (RalGDS/AF-6) domain family (N-terminal) member 8 (RASSF8) transcript variant 2 |
CNY 6,750.00 |