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MMEL1 (NM_033467) Human Tagged ORF Clone
CAT＃: RG229371
- TrueORF®
MMEL1 (tGFP-tagged) - Human membrane metallo-endopeptidase-like 1 (MMEL1)
ORF Plasmid: DDK
"NM_033467" in other vectors (5)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | MMEL2; NEP2; NEPII; NL1; NL2; SEP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG229371 representing NM_033467
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGAAGTCCGAAGGCCCAGTGGGGATGGTGGAGAGCGCCGGCCGTGCAGGGCAGAAGCGCCCGGGGT TCCTGGAGGGGGGGCTGCTGCTGCTGCTGCTGCTGGTGACCGCTGCCCTGGTGGCCTTGGGTGTCCTCTA CGCCGACCGCAGAGGGAAGCAGCTGCCACGCCTTGCTAGCCGGCTGTGCTTCTTACAGGAGGAGAGGACC TTTGTAAAACGAAAACCCCGAGGGATCCCAGAGGCCCAAGAGGTGAGCGAGGTCTGCACCACCCCTGGCT GCGTGATAGCAGCTGCCAGGATCCTCCAGAACATGGACCCGACCACGGAACCGTGTGACGACTTCTACCA GTTTGCATGCGGAGGCTGGCTGCGGCGCCACGTGATCCCTGAGACCAACTCAAGATACAGCATCTTTGAC GTCCTCCGCGACGAGCTGGAGGTCATCCTCAAAGCGGTGCTGGAGAATTCGACTGCCAAGGACCGGCCGG CTGTGGAGAAGGCCAGGACGCTGTACCGCTCCTGCATGAACCAGAGTGTGATAGAGAAGCGAGGCTCTCA GCCCCTGCTGGACATCTTGGAGGTGGTGGGAGGCTGGCCGGTGGCGATGGACAGGTGGAACGAGACCGTA GGACTCGAGTGGGAGCTGGAGCGGCAGCTGGCGCTGATGAACTCACAGTTCAACAGGCGCGTCCTCATCG ACCTCTTCATCTGGAACGACGACCAGAACTCCAGCCGGCACATCATCTACATAGACCAGCCCACCTTGGG CATGCCCTCCCGAGAGTACTACTTCAACGGCGGCAGCAACCGGAAGGTGCGGGAAGCCTACCTGCAGTTC ATGGTGTCAGTGGCCACGTTGCTGCGGGAGGATGCAAACCTGCCCAGGGACAGCTGCCTGGTGCAGGAGG ACATGGTGCAGGTGCTGGAGCTGGAGACACAGCTGGCCAAGGCCACGGTACCCCAGGAGGAGAGACACGA CGTCATCGCCTTGTACCACCGGATGGGACTGGAGGAGCTGCAAAGCCAGTTTGGCCTGAAGGGATTTAAC TGGACTCTGTTCATACAAACTGTGCTATCCTCTGTCAAAATCAAGCTGCTGCCAGATGAGGAAGTGGTGG TCTATGGCATCCCCTACCTGCAGAACCTTGAAAACATCATCGACACCTACTCAGCCAGGACCATACAGAA CTACCTGGTCTGGCGCCTGGTGCTGGACCGCATTGGTAGCCTAAGCCAGAGATTCAAGGACACACGAGTG AACTACCGCAAGGCGCTGTTTGGCACAATGGTGGAGGAGGTGCGCTGGCGTGAATGTGTGGGCTACGTCA ACAGCAACATGGAGAACGCCGTGGGCTCCCTCTACGTCAGGGAGGCGTTCCCTGGAGACAGCAAGAGCAT GGTCAGAGAACTCATTGACAAGGTGCGGACAGTGTTTGTGGAGACGCTGGACGAGCTGGGCTGGATGGAC GAGGAGTCCAAGAAGAAGGCGCAGGAGAAGGCCATGAGCATCCGGGAGCAGATCGGGCACCCTGACTACA TCCTGGAGGAGATGAACAGGCGCCTGGACGAGGAGTACTCCAATCTGAACTTCTCAGAGGACCTGTACTT TGAGAACAGTCTGCAGAACCTCAAGGTGGGCGCCCAGCGGAGCCTCAGGAAGCTTCGGGAAAAGGTGGAC CCAAATCTCTGGATCATCGGGGCGGCGGTGGTCAATGCGTTCTACTCCCCAAACCGAAACCAGATTGTAT TCCCTGCCGGGATCCTCCAGCCCCCCTTCTTCAGCAAGGAGCAGCCACAGGCCTTGAACTTTGGAGGCAT TGGGATGGTGATCGGGCACGAGATCACGCACGGCTTTGACGACAATGGCCGGAACTTCGACAAGAATGGC AACATGATGGATTGGTGGAGTAACTTCTCCACCCAGCACTTCCGGGAGCAGTCAGAGTGCATGATCTACC AGTACGGCAACTACTCCTGGGACCTGGCAGACGAACAGAACGTGAACGGATTCAACACCCTTGGGGAAAA CATTGCTGACAACGGAGGGGTGCGGCAAGCCTATAAGGCCTACCTCAAGTGGATGGCAGAGGGTGGCAAG GACCAGCAGCTGCCCGGCCTGGATCTCACCCATGAGCAGCTCTTCTTCATCAACTATGCCCAGGTGTGGT GCGGGTCCTACCGGCCCGAGTTCGCCATCCAATCCATCAAGACAGACGTCCACAGTCCCCTGAAGTACAG GGTACTGGGGTCGCTGCAGAACCTGGCCGCCTTCGCAGACACGTTCCACTGTGCCCGGGGCACCCCCATG CACCCCAAGGAGCGATGCCGCGTGTGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG229371 representing NM_033467
Red=Cloning site Green=Tags(s) MGKSEGPVGMVESAGRAGQKRPGFLEGGLLLLLLLVTAALVALGVLYADRRGKQLPRLASRLCFLQEERT FVKRKPRGIPEAQEVSEVCTTPGCVIAAARILQNMDPTTEPCDDFYQFACGGWLRRHVIPETNSRYSIFD VLRDELEVILKAVLENSTAKDRPAVEKARTLYRSCMNQSVIEKRGSQPLLDILEVVGGWPVAMDRWNETV GLEWELERQLALMNSQFNRRVLIDLFIWNDDQNSSRHIIYIDQPTLGMPSREYYFNGGSNRKVREAYLQF MVSVATLLREDANLPRDSCLVQEDMVQVLELETQLAKATVPQEERHDVIALYHRMGLEELQSQFGLKGFN WTLFIQTVLSSVKIKLLPDEEVVVYGIPYLQNLENIIDTYSARTIQNYLVWRLVLDRIGSLSQRFKDTRV NYRKALFGTMVEEVRWRECVGYVNSNMENAVGSLYVREAFPGDSKSMVRELIDKVRTVFVETLDELGWMD EESKKKAQEKAMSIREQIGHPDYILEEMNRRLDEEYSNLNFSEDLYFENSLQNLKVGAQRSLRKLREKVD PNLWIIGAAVVNAFYSPNRNQIVFPAGILQPPFFSKEQPQALNFGGIGMVIGHEITHGFDDNGRNFDKNG NMMDWWSNFSTQHFREQSECMIYQYGNYSWDLADEQNVNGFNTLGENIADNGGVRQAYKAYLKWMAEGGK DQQLPGLDLTHEQLFFINYAQVWCGSYRPEFAIQSIKTDVHSPLKYRVLGSLQNLAAFADTFHCARGTPM HPKERCRVW TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_033467 |
| ORF Size | 2337 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_033467.4 |
| RefSeq Size | 2902 bp |
| RefSeq ORF | 2340 bp |
| Locus ID | 79258 |
| UniProt ID | Q495T6 |
| Protein Families | Druggable Genome, Protease, Secreted Protein, Transmembrane |
| Gene Summary | The protein encoded by this gene is a member of the neutral endopeptidase (NEP) or membrane metallo-endopeptidase (MME) family. Family members play important roles in pain perception, arterial pressure regulation, phosphate metabolism and homeostasis. This protein is a type II transmembrane protein and is thought to be expressed as a secreted protein. This gene is expressed mainly in testis with weak expression in the brain, kidney, and heart. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC229371 | MMEL1 (Myc-DDK-tagged)-Human membrane metallo-endopeptidase-like 1 (MMEL1) |
CNY 5,704.00 |
|
| RC229371L3 | Lenti-ORF clone of MMEL1 (Myc-DDK-tagged)-Human membrane metallo-endopeptidase-like 1 (MMEL1) |
CNY 5,890.00 |
|
| RC229371L4 | Lenti-ORF clone of MMEL1 (mGFP-tagged)-Human membrane metallo-endopeptidase-like 1 (MMEL1) |
CNY 5,890.00 |
|
| SC123914 | MMEL1 (untagged)-Human membrane metallo-endopeptidase-like 1 (MMEL1) |
CNY 7,220.00 |
|
| SC328006 | MMEL1 (untagged)-Human membrane metallo-endopeptidase-like 1 (MMEL1) |
CNY 7,220.00 |
