MRRF (NM_001173512) Human Tagged ORF Clone
CAT#: RG229688
- TrueORF®
MRRF (tGFP-tagged) - Human mitochondrial ribosome recycling factor (MRRF), nuclear gene encoding mitochondrial protein, transcript variant 3
ORF Plasmid: DDK
"NM_001173512" in other vectors (4)
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CNY 4,370.00
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | MRFF; MTRRF; RRF |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG229688 representing NM_001173512
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCTTGGGATTAAAGTGCTTCCGCATGGTCCACCCTACCTTTCGCAATTATCTTGCAGCCTCTATCA GACCCGTTTCAGAAGTTACACTGAAGACAGTGCATGAAAGACAACATGGCCATAGGCAATACATGGCCTA TTCAGCTGTACCAGTCCGCCATTTTGCTACCAAGAAAGCCAAAGGATCCCTTGACAAGATTGCTGTGGTA ACTGCTGACGGGAAGCTTGCTTTAAACCAGATTAGCCAGATCTCCATGAAGTCGCCACAGCTGATTTTGG TGAATATGGCCAGCTTCCCAGAGTGTACAGCTGCAGCTATCAAGGCTATAAGAGAAAGTGGAATGAATCT GAACCCAGAAGTGGAAGGGACGCTAATTCGGGTACCCATTCCCCAAGTAACCAGAGAGCACAGAGAAATG CTGGTGAAACTGGCCAAACAGAACACCAACAAGGCCAAAGACTCTTTACGGAAGGTTCGCACCAACTCAA TGAACAAGCTGAAGAAATCCAAGGATACAGTCTCAGAGGACACCATTAGGCTAATAGAGAAACAGATCAG CCAAATGGCCGATGACACAGTGGCAGAACTGGACAGGCATCTGGCAGTGAAGACCAAAGAACTCCTTGGA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG229688 representing NM_001173512
Red=Cloning site Green=Tags(s) MALGLKCFRMVHPTFRNYLAASIRPVSEVTLKTVHERQHGHRQYMAYSAVPVRHFATKKAKGSLDKIAVV TADGKLALNQISQISMKSPQLILVNMASFPECTAAAIKAIRESGMNLNPEVEGTLIRVPIPQVTREHREM LVKLAKQNTNKAKDSLRKVRTNSMNKLKKSKDTVSEDTIRLIEKQISQMADDTVAELDRHLAVKTKELLG TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001173512 |
ORF Size | 630 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001173512.2 |
RefSeq Size | 1880 bp |
RefSeq ORF | 633 bp |
Locus ID | 92399 |
UniProt ID | Q96E11 |
Protein Families | Transmembrane |
Gene Summary | This gene encodes a ribosome recycling factor, which is a component of the mitochondrial translational machinery. The encoded protein, along with mitochondrial elongation factor 2, functions in ribosomal recycling at the termination of mitochondrial translation by mediating the disassembly of ribosomes from messenger RNA. A pseudogene of this gene has been identified on chromosome X. [provided by RefSeq, Oct 2016] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC229688 | MRRF (Myc-DDK-tagged)-Human mitochondrial ribosome recycling factor (MRRF), nuclear gene encoding mitochondrial protein, transcript variant 3 |
CNY 3,990.00 |
|
RC229688L3 | Lenti-ORF clone of MRRF (Myc-DDK-tagged)-Human mitochondrial ribosome recycling factor (MRRF), nuclear gene encoding mitochondrial protein, transcript variant 3 |
CNY 5,890.00 |
|
RC229688L4 | Lenti-ORF clone of MRRF (mGFP-tagged)-Human mitochondrial ribosome recycling factor (MRRF), nuclear gene encoding mitochondrial protein, transcript variant 3 |
CNY 5,890.00 |
|
SC328326 | MRRF (untagged)-Human mitochondrial ribosome recycling factor (MRRF) nuclear gene encoding mitochondrial protein transcript variant 3 |
CNY 3,990.00 |