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LMX1A (NM_001174069) Human Tagged ORF Clone
CAT＃: RG229968
- TrueORF®
LMX1A (tGFP-tagged) - Human LIM homeobox transcription factor 1, alpha (LMX1A), transcript variant 4
ORF Plasmid: DDK
"NM_001174069" in other vectors (6)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | DFNA7; LMX1; LMX1.1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG229968 representing NM_001174069
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGGACGGCCTAAAGATGGAGGAGAACTTCCAAAGCGCGATCGACACCTCGGCCTCCTTCTCCTCGC TGCTGGGCAGAGCGGTGAGCCCCAAGTCTGTCTGCGAGGGCTGTCAGCGGGTCATCTTGGACAGGTTTCT GCTGCGGCTCAACGACAGCTTCTGGCATGAGCAGTGCGTGCAGTGCGCCTCCTGCAAAGAGCCCCTGGAG ACCACCTGCTTCTACCGGGACAAGAAGCTGTACTGCAAGTATGACTACGAGAAGCTGTTTGCTGTTAAAT GTGGGGGCTGCTTCGAGGCCATCGCTCCCAATGAGTTTGTTATGCGGGCCCAGAAGAGTGTATACCACCT GAGCTGCTTCTGCTGCTGTGTCTGCGAGCGACAGCTTCAGAAGGGTGATGAGTTTGTCCTGAAGGAGGGG CAGCTGCTCTGCAAAGGGGACTATGAGAAGGAGCGGGAGCTGCTCAGCCTGGTGAGCCCAGCAGCCTCAG ACTCAGGTAAAAGTGATGATGAAGAAAGTCTCTGCAAGTCAGCCCATGGGGCAGGGAAAGGAACTGCTGA GGAAGGCAAGGACCATAAGCGCCCCAAACGTCCGAGAACCATCTTGACAACTCAACAGAGGCGAGCATTC AAGGCCTCATTTGAAGTATCCTCCAAGCCCTGCAGGAAGGTGAGAGAGACTCTGGCTGCAGAGACAGGGC TGAGTGTCCGTGTCGTCCAGGTGTGGTTCCAAAACCAGAGAGCGAAGATGAAGAAGCTGGCCAGGCGACA GCAGCAGCAGCAGCAAGATCAGCAGAACACCCAGAGGCTGAGCTCTGCTCAGACAAACGGTGGTGGGAGT GCTGGGATGGAAGGAATCATGAACCCCTACACGGCTCTGCCCACCCCACAGCAGCTCCTGGCCATCGAGC AGAGTGTCTACAGCTCAGATCCCTTCCGACAGGGTCTCACCCCACCCCAGATGCCTGGAGACCACATGCA CCCTTATGGTGCCGAGCCCCTTTTCCATGACCTGGATAGCGACGACACCTCCCTCAGTAACCTGGGTGAT TGTTTCCTAGCAACCTCAGAAGCTGGGCCTCTGCAGTCCAGAGTGGGAAACCCCATTGACCATCTGTACT CCATGCAGAATTCTTACTTCACATCT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG229968 representing NM_001174069
Red=Cloning site Green=Tags(s) MLDGLKMEENFQSAIDTSASFSSLLGRAVSPKSVCEGCQRVILDRFLLRLNDSFWHEQCVQCASCKEPLE TTCFYRDKKLYCKYDYEKLFAVKCGGCFEAIAPNEFVMRAQKSVYHLSCFCCCVCERQLQKGDEFVLKEG QLLCKGDYEKERELLSLVSPAASDSGKSDDEESLCKSAHGAGKGTAEEGKDHKRPKRPRTILTTQQRRAF KASFEVSSKPCRKVRETLAAETGLSVRVVQVWFQNQRAKMKKLARRQQQQQQDQQNTQRLSSAQTNGGGS AGMEGIMNPYTALPTPQQLLAIEQSVYSSDPFRQGLTPPQMPGDHMHPYGAEPLFHDLDSDDTSLSNLGD CFLATSEAGPLQSRVGNPIDHLYSMQNSYFTS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001174069 |
| ORF Size | 1146 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001174069.1, NP_001167540.1 |
| RefSeq Size | 3366 bp |
| RefSeq ORF | 1149 bp |
| Locus ID | 4009 |
| UniProt ID | Q8TE12 |
| Protein Families | ES Cell Differentiation/IPS, Transcription Factors |
| Gene Summary | This gene encodes a homeodomain and LIM-domain containing protein. The encoded protein is a transcription factor that acts as a positive regulator of insulin gene transcription. This gene also plays a role in the development of dopamine producing neurons during embryogenesis. Mutations in this gene are associated with an increased risk of developing Parkinson's disease. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Feb 2012] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC229968 | LMX1A (Myc-DDK-tagged)-Human LIM homeobox transcription factor 1, alpha (LMX1A), transcript variant 4 |
CNY 3,656.00 |
|
| RC229968L1 | Lenti ORF clone of Human LIM homeobox transcription factor 1, alpha (LMX1A), transcript variant 4, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC229968L2 | Lenti ORF clone of Human LIM homeobox transcription factor 1, alpha (LMX1A), transcript variant 4, mGFP tagged |
CNY 5,890.00 |
|
| RC229968L3 | Lenti ORF clone of Human LIM homeobox transcription factor 1, alpha (LMX1A), transcript variant 4, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC229968L4 | Lenti ORF clone of Human LIM homeobox transcription factor 1, alpha (LMX1A), transcript variant 4, mGFP tagged |
CNY 5,890.00 |
|
| SC328606 | LMX1A (untagged)-Human LIM homeobox transcription factor 1 alpha (LMX1A) transcript variant 4 |
CNY 3,656.00 |
