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RIM1 (RIMS1) (NM_001168409) Human Tagged ORF Clone
CAT＃: RG230481
- TrueORF®
RIMS1 (tGFP-tagged) - Human regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant 4
ORF Plasmid: DDK
"NM_001168409" in other vectors (4)
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CNY 8,930.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CORD7; RAB3IP2; RIM; RIM1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG230481 representing NM_001168409
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCAAAGACTCAGGTGCATTGCTGGGTCTGAAAGTTGTTGGAGGAAAAATGACTGACTTAGGACGAC TTGGTGCTTTCATCACCAAAGTAAAGAAGGGTAGCCTAGCAGATGTAGTTGGACACCTAAGAGCAGGGGA TGAAGTTCTAGAATGGAATGGTAAACCCCTGCCGGGAGCTACAAATGAAGAAGTTTACAACATTATTTTA GAATCAAAATCAGAACCTCAAGTTGAAATTATTGTTTCAAGGCCTATTGGTGACATTCCCCGGATTCCTG AGAGCTCCCACCCTCCACTGGAGTCCAGTTCAAGTTCCTTTGAATCTCAGAAGATGGAAAGGCCTTCCAT TTCTGTTATTTCTCCAACAAGTCCTGGAGCTCTAAAAGATGCCCCACAAGTCTTACCAGGGCAACTTTCT GTGAAGTTGTGGTATGATAAAGTGGGACACCAGCTGATTGTAAATGTTCTGCAAGCAACAGATCTACCTG CTAGAGTAGATGGACGTCCTCGAAATCCCTATGTAAAAATGTATTTTCTTCCAGATAGAAGTGATAAAAG TAAAAGGAGGACCAAAACAGTAAAGAAAATACTAGAACCAAAATGGAATCAAACTTTTGTCTATTCACAT GTACATCGTAGAGATTTTAGAGAACGAATGTTAGAAATAACTGTGTGGGACCAACCAAGAGTGCAAGAAG AAGAAAGTGAATTTCTTGGAGAGATCCTCATAGAATTGGAGACAGCGCTTTTAGATGATGAACCGCATTG GTATAAACTTCAGACACATGATGAGTCTTCACTACCTCTGCCTCAGCCATCACCTTTCATGCCAAGGCGA CATATTCATGGAGAAAGCTCTAGCAAAAAGCTACAAAGATCTCAGCGAATCAGTGATAGTGACATCTCAG ATTATGAGGTTGATGATGGTATTGGCGTAGTTCCTCCAGTAGGCTATAGGTCTAGTGCTAGAGAAAGTAA ATCTACAACATTAACTGTGCCAGAACAGCAAAGAACAACTCATCACCGCTCACGTTCAGTATCTCCTCAT CGCGGCAATGATCAGGGAAAGCCGCGTTCACGTTTACCAAATGTGCCATTACAGAGGAGTTTAGATGAAA TTCATCCAACAAGAAGGTCACGTTCTCCAACCAGACACCATGATGCCTCCCGAAGTCCAGTTGATCATAG AACCAGAGATGTGGATAGTCAGTATTTATCAGAACAAGACAGTGAGCTTCTTATGCTGCCCAGAGCAAAA CGAGGACGAAGTGCAGAATGCCTACATACTACCAGTGAACTGCAGCCCTTTCTTGACAGGGCTAGGAGTG CTAGTACCAACTGCTTGAGACCAGATACTAGTTTGCATTCACCAGAACGAGAAAGGCACTCCAGAAAGTC TGAAAGATCTAGCATCCAAAAACAGACTAGGAAAGGCACTGCCTCTGATGCAGAAAGAATGCACCGACAG AGAAGTCCAACACAATCTCCTCCAGCAGACACATCGTTCAGCAGTCGCAGGGGAAGACAGCTCCCACAAG TGCCAGTGAGAAGCGGCAGTATAGAACAAGAGTCGGGCCACAAAAAGTTAAAAAGTACCATCCAGAGAAG CACAGAAACAGGCATGGCAGCTGAAATGAGAAAGATGGTAAGGCAGCCGAGCCGAGAGTCTACTGATGGC AGCATCAACAGTTACAGCTCTGAGGGCAATTTAATATTTCCTGGAGTGCGACTGGGAGCTGACAGTCAAT TCAGTGATTTTCTTGATGGATTGGGACCAGCCCAGCTTGTTGGCCGCCAAACCCTTGCCACCCCTGCAAT GGGTGATATACAAATAGGAATGGAGGACAAAAAGGGCCAATTAGAAGTGGAAGTCATTAGAGCACGAAGC CTCACACAAAAGCCTGGTTCCAAATCTACACCTGCTCCATATGTCAAAGTATATCTTTTGGAAAATGGGG CCTGTATAGCCAAGAAGAAGACAAGAATTGCACGAAAAACCCTTGATCCTTTGTATCAGCAGTCTCTGGT TTTTGATGAAAGTCCACAGGGTAAAGTTCTTCAGGTGATTGTCTGGGGAGACTATGGCAGAATGGACCAC AAATGCTTTATGGGTGTGGCTCAGATCTTGTTGGAAGAACTCGACCTGTCCAGCATGGTGATCGGATGGT ACAAATTGTTCCCACCGTCCTCACTGGTGGATCCCACACTCACTCCCCTCACCCGGCGGGCTTCCCAGTC ATCTCTGGAAAGTTCAACTGGGCCTCCCTGTATTCGATCA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG230481 representing NM_001168409
Red=Cloning site Green=Tags(s) MPKDSGALLGLKVVGGKMTDLGRLGAFITKVKKGSLADVVGHLRAGDEVLEWNGKPLPGATNEEVYNIIL ESKSEPQVEIIVSRPIGDIPRIPESSHPPLESSSSSFESQKMERPSISVISPTSPGALKDAPQVLPGQLS VKLWYDKVGHQLIVNVLQATDLPARVDGRPRNPYVKMYFLPDRSDKSKRRTKTVKKILEPKWNQTFVYSH VHRRDFRERMLEITVWDQPRVQEEESEFLGEILIELETALLDDEPHWYKLQTHDESSLPLPQPSPFMPRR HIHGESSSKKLQRSQRISDSDISDYEVDDGIGVVPPVGYRSSARESKSTTLTVPEQQRTTHHRSRSVSPH RGNDQGKPRSRLPNVPLQRSLDEIHPTRRSRSPTRHHDASRSPVDHRTRDVDSQYLSEQDSELLMLPRAK RGRSAECLHTTSELQPFLDRARSASTNCLRPDTSLHSPERERHSRKSERSSIQKQTRKGTASDAERMHRQ RSPTQSPPADTSFSSRRGRQLPQVPVRSGSIEQESGHKKLKSTIQRSTETGMAAEMRKMVRQPSRESTDG SINSYSSEGNLIFPGVRLGADSQFSDFLDGLGPAQLVGRQTLATPAMGDIQIGMEDKKGQLEVEVIRARS LTQKPGSKSTPAPYVKVYLLENGACIAKKKTRIARKTLDPLYQQSLVFDESPQGKVLQVIVWGDYGRMDH KCFMGVAQILLEELDLSSMVIGWYKLFPPSSLVDPTLTPLTRRASQSSLESSTGPPCIRS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001168409 |
| ORF Size | 2280 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001168409.2 |
| RefSeq Size | 5059 bp |
| RefSeq ORF | 2283 bp |
| Locus ID | 22999 |
| UniProt ID | Q86UR5 |
| Gene Summary | The protein encoded by this gene is a RAS gene superfamily member that regulates synaptic vesicle exocytosis. This gene also plays a role in the regulation of voltage-gated calcium channels during neurotransmitter and insulin release. Mutations have suggested a role cognition and have been identified as the cause of cone-rod dystrophy type 7. Multiple transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Mar 2012] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC230481 | RIMS1 (Myc-DDK-tagged)-Human regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant 4 |
CNY 8,080.00 |
|
| RC230481L3 | Lenti-ORF clone of RIMS1 (Myc-DDK-tagged)-Human regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant 4 |
CNY 9,980.00 |
|
| RC230481L4 | Lenti-ORF clone of RIMS1 (mGFP-tagged)-Human regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant 4 |
CNY 9,980.00 |
|
| SC329119 | RIMS1 (untagged)-Human regulating synaptic membrane exocytosis 1 (RIMS1) transcript variant 4 |
CNY 14,730.00 |
