Selenophosphate synthetase 1 (SEPHS1) (NM_001195602) Human Tagged ORF Clone
CAT#: RG231163
- TrueORF®
SEPHS1 (tGFP-tagged) - Human selenophosphate synthetase 1 (SEPHS1), transcript variant 2
ORF Plasmid: DDK
"NM_001195602" in other vectors (3)
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CNY 4,370.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | SELD; SPS; SPS1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG231163 representing NM_001195602
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATACTTGTGTCATTCCTTTGAGGCACGGTGGGCTTTCCTTGGTTCAAACCACAGATTACATTTACC CGATCGTAGACGACCCTTACATGATGGGCAGGATAGCGTGTGCCAATGTCCTCAGTGACCTCTATGCAAT GGGGGTCACGGAATGTGACAATATGCTGATGCTCCTTGGAGTCAGTAATAAAATGACCGACAGGGAAAGG GATAAAGTGATGCCTCTGATTATCCAAGGTTTTAAAGACGCAGCTGAGGAAGCAGGAACATCTGTAACAG GCGGCCAAACAGTACTAAACCCCTGGATTGTCCTGGGAGGAGTGGCTACCACTGTCTGCCAACCCAATGA ATTTATCATGCCAGACAATGCAGTGCCAGGGGACGTGCTGGTGCTGACAAAACCCCTGGGGACACAGGTG GCAGTGGCTGTGCACCAGTGGCTGGATATCCCTGAGAAATGGAATAAGATTAAACTAGTGGTCACCCAAG AAGATGTAGAGCTGGCCTACCAGGAGGCGATGATGAACATGGCGAGGCTCAACAGGACAGCTGCAGGACT CATGCACACGTTCAATGCCCACGCCGCCACTGACATCACGGGCTTCGGGATTTTGGGCCATGCGCAGAAC CTGGCCAAGCAGCAGAGGAACGAGGTGTCGTTTGTAATTCACAACCTCCCGGTGCTGGCCAAGATGGCTG CGGTGAGCAAGGCCTGCGGAAACATGTTCGGCCTCATGCACGGGACCTGCCCGGAGACTTCAGGCGGCCT TCTGATCTGTTTACCACGTGAGCAAGCAGCTCGGTTCTGTGCAGAGATAAAGTCCCCCAAATATGGTGAA GGCCACCAAGCATGGATTATTGGGATTGTAGAGAAGGGCAACCGCACAGCCAGAATCATAGACAAACCCC GGATCATCGAGGTCGCACCACAAGTGGCCACTCAAAATGTGAATCCCACACCCGGGGCCACCTCT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG231163 representing NM_001195602
Red=Cloning site Green=Tags(s) MDTCVIPLRHGGLSLVQTTDYIYPIVDDPYMMGRIACANVLSDLYAMGVTECDNMLMLLGVSNKMTDRER DKVMPLIIQGFKDAAEEAGTSVTGGQTVLNPWIVLGGVATTVCQPNEFIMPDNAVPGDVLVLTKPLGTQV AVAVHQWLDIPEKWNKIKLVVTQEDVELAYQEAMMNMARLNRTAAGLMHTFNAHAATDITGFGILGHAQN LAKQQRNEVSFVIHNLPVLAKMAAVSKACGNMFGLMHGTCPETSGGLLICLPREQAARFCAEIKSPKYGE GHQAWIIGIVEKGNRTARIIDKPRIIEVAPQVATQNVNPTPGATS TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001195602 |
ORF Size | 975 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001195602.1, NP_001182531.1 |
RefSeq Size | 3004 bp |
RefSeq ORF | 978 bp |
Locus ID | 22929 |
UniProt ID | P49903 |
Protein Families | Stem cell - Pluripotency |
Protein Pathways | Metabolic pathways, Selenoamino acid metabolism |
Gene Summary | This gene encodes an enzyme that synthesizes selenophosphate from selenide and ATP. Selenophosphate is the selenium donor used to synthesize selenocysteine, which is co-translationally incorporated into selenoproteins at in-frame UGA codons. [provided by RefSeq, Sep 2010] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC231163 | SEPHS1 (Myc-DDK-tagged)-Human selenophosphate synthetase 1 (SEPHS1), transcript variant 2 |
CNY 2,400.00 |
|
RC231163L3 | Lenti-ORF clone of SEPHS1 (Myc-DDK-tagged)-Human selenophosphate synthetase 1 (SEPHS1), transcript variant 2 |
CNY 5,890.00 |
|
RC231163L4 | Lenti-ORF clone of SEPHS1 (mGFP-tagged)-Human selenophosphate synthetase 1 (SEPHS1), transcript variant 2 |
CNY 5,890.00 |
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