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USP2 (NM_001243759) Human Tagged ORF Clone
CAT＃: RG232579
- TrueORF®
USP2 (tGFP-tagged) - Homo sapiens ubiquitin specific peptidase 2 (USP2), transcript variant 3
ORF Plasmid: DDK
"NM_001243759" in other vectors (2)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | UBP41; USP9 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG232579 representing NM_001243759
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTTGTGCCCGGTTCGACTCGTCCATACTCCAAGAAGAGGCAGAATTCTAAGAGTGCCCAGGGTCTGG CTGGTCTTCGAAACCTTGGGAACACGTGCTTCATGAACTCAATTCTGCAGTGCCTGAGCAACACTCGGGA GTTGAGAGATTACTGCCTCCAGAGGCTCTACATGCGGGACCTGCACCACGGCAGCAATGCACACACAGCC CTCGTGGAAGAGTTTGCAAAACTAATTCAGACCATATGGACTTCATCCCCCAATGATGTGGTGAGCCCAT CTGAGTTCAAGACCCAGATCCAGAGATACGCACCGCGCTTTGTTGGCTATAATCAGCAGGATGCTCAGGA GTTCCTTCGCTTTCTTCTGGATGGGCTCCATAACGAGGTGAACCGAGTGACACTGAGACCTAAGTCCAAC CCTGAGAACCTCGATCATCTTCCTGATGACGAGAAAGGCCGACAGATGTGGAGAAAATATCTAGAACGGG AAGACAGTAGGATCGGGGATCTCTTTGTTGGGCAGCTAAAGAGCTCGCTGACGTGTACAGATTGTGGTTA CTGTTCTACGGTCTTCGACCCCTTCTGGGACCTCTCACTGCCCATTGCTAAGCGAGGTTATCCTGAGGTG ACATTAATGGACTGCATGAGGCTCTTCACCAAAGAGGATGTGCTTGATGGAGATGAAAAGCCAACATGCT GTCGCTGCCGAGGCAGAAAACGGTGTATAAAGAAGTTCTCCATCCAGAGGTTCCCAAAGATCTTGGTGCT CCATCTGAAGCGGTTCTCAGAATCCAGGATCCGAACCAGCAAGCTCACAACATTTGTGAACTTCCCCCTA AGAGACCTGGACTTAAGAGAATTTGCCTCAGAAAACACCAACCATGCTGTTTACAACCTGTACGCTGTGT CCAATCACTCCGGAACCACCATGGGTGGCCACTATACAGCCTACTGTCGCAGTCCAGGGACAGGAGAATG GCACACTTTCAACGACTCCAGCGTCACTCCCATGTCCTCCAGCCAAGTGCGCACCAGCGACGCCTACCTG CTCTTCTACGAACTGGCCAGCCCGCCCTCCCGAATG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG232579 representing NM_001243759
Red=Cloning site Green=Tags(s) MLVPGSTRPYSKKRQNSKSAQGLAGLRNLGNTCFMNSILQCLSNTRELRDYCLQRLYMRDLHHGSNAHTA LVEEFAKLIQTIWTSSPNDVVSPSEFKTQIQRYAPRFVGYNQQDAQEFLRFLLDGLHNEVNRVTLRPKSN PENLDHLPDDEKGRQMWRKYLEREDSRIGDLFVGQLKSSLTCTDCGYCSTVFDPFWDLSLPIAKRGYPEV TLMDCMRLFTKEDVLDGDEKPTCCRCRGRKRCIKKFSIQRFPKILVLHLKRFSESRIRTSKLTTFVNFPL RDLDLREFASENTNHAVYNLYAVSNHSGTTMGGHYTAYCRSPGTGEWHTFNDSSVTPMSSSQVRTSDAYL LFYELASPPSRM TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001243759 |
| ORF Size | 1086 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001243759.2 |
| RefSeq Size | 2933 bp |
| RefSeq ORF | 1089 bp |
| Locus ID | 9099 |
| UniProt ID | O75604 |
| Protein Families | Protease |
| Gene Summary | This gene encodes a member of the family of de-ubiquitinating enzymes, which belongs to the peptidase C19 superfamily. The encoded protein is a ubiquitin-specific protease which is required for TNF-alpha (tumor necrosis factor alpha) -induced NF-kB (nuclear factor kB) signaling. This protein deubiquitinates polyubiquitinated target proteins such as fatty acid synthase, murine double minute 2 (MDM2), MDM4/MDMX and cyclin D1. MDM2 and MDM4 are negative regulators of the p53 tumor suppressor and cyclin D1 is required for cell cycle G1/S transition. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011] |
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