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LHX6 (NM_001242334) Human Tagged ORF Clone
CAT＃: RG232583
- TrueORF®
LHX6 (tGFP-tagged) - Homo sapiens LIM homeobox 6 (LHX6), transcript variant 4
ORF Plasmid: DDK
"NM_001242334" in other vectors (2)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | LHX6.1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG232583 representing NM_001242334
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCAGCCAGGGTCCGGCTGCAAAGCGACCACCCGCTGTCTTGAAGGGACCGCGCCGCCCGCCATGG CTCAGTCTGACGCCGAGGCCCTGGCAGGAGCTCTGGACAAGGACGAGGGTCAGGCCTCCCCATGTACGCC CAGCACGCCATCTGTCTGCTCACCGCCCTCTGCCGCCTCCTCCGTGCCGTCTGCAGGCAAGAACATCTGC TCCAGCTGCGGCCTCGAGATCCTGGACCGATATCTGCTCAAGGTCAACAACCTCATCTGGCACGTGCGGT GCCTCGAGTGCTCCGTGTGTCGCACGTCGCTGAGGCAGCAGAACAGCTGCTACATCAAGAACAAGGAGAT CTTCTGCAAGATGGACTACTTCAGCCGATTCGGGACCAAGTGTGCCCGGTGCGGCCGACAGATCTACGCC AGCGACTGGGTGCGGAGAGCTCGCGGCAACGCCTACCACCTGGCCTGCTTCGCCTGCTTCTCGTGCAAGC GCCAGCTGTCCACTGGTGAGGAGTTCGGCCTGGTCGAGGAGAAGGTGCTCTGCCGCATCCACTACGACAC CATGATTGAGAACCTCAAGAGGGCCGCCGAGAACGGGAACGGCCTCACGTTGGAGGGGGCAGTGCCCTCG GAACAGGACAGTCAACCCAAGCCGGCCAAGCGCGCGCGGACGTCCTTCACCGCGGAACAGCTGCAGGTTA TGCAGGCGCAGTTCGCGCAGGACAACAACCCCGACGCTCAGACGCTGCAGAAGCTGGCGGACATGACGGG CCTCAGCCGGAGAGTCATCCAGGTGTGGTTTCAAAACTGCCGGGCGCGTCATAAAAAGCACACGCCGCAA CACCCAGTGCCGCCCTCGGGGGCGCCCCCGTCCCGCCTTCCCTCCGCCCTGTCCGACGACATCCACTACA CCCCGTTCAGCAGCCCCGAGCGGGCGCGCATGGTCACCCTGCACGGCTACATTGAGAGTCAGGTACAGTG CGGGCAGGTGCACTGCCGGCTGCCTTACACCGCACCCCCCGTCCACCTCAAAGCCGATATGGATGGGCCG CTCTCCAACCGGGGTGAGAAGGTCATCCTTTTTCAGTAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG232583 representing NM_001242334
Red=Cloning site Green=Tags(s) MAQPGSGCKATTRCLEGTAPPAMAQSDAEALAGALDKDEGQASPCTPSTPSVCSPPSAASSVPSAGKNIC SSCGLEILDRYLLKVNNLIWHVRCLECSVCRTSLRQQNSCYIKNKEIFCKMDYFSRFGTKCARCGRQIYA SDWVRRARGNAYHLACFACFSCKRQLSTGEEFGLVEEKVLCRIHYDTMIENLKRAAENGNGLTLEGAVPS EQDSQPKPAKRARTSFTAEQLQVMQAQFAQDNNPDAQTLQKLADMTGLSRRVIQVWFQNCRARHKKHTPQ HPVPPSGAPPSRLPSALSDDIHYTPFSSPERARMVTLHGYIESQVQCGQVHCRLPYTAPPVHLKADMDGP LSNRGEKVILFQY TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001242334 |
| ORF Size | 1089 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001242334.2 |
| RefSeq Size | 3394 bp |
| RefSeq ORF | 1092 bp |
| Locus ID | 26468 |
| UniProt ID | Q9UPM6 |
| Protein Families | Transcription Factors |
| Gene Summary | This gene encodes a member of a large protein family that contains the LIM domain, a unique cysteine-rich zinc-binding domain. The encoded protein has tandem LIM domains as well as a DNA-binding homeodomain. The protein functions as a transcription factor involved in embryogenesis and head development and is highly expressed in neural crest derived mesenchyme cells. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jan 2017] |
Documents
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