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TEA domain family member 2 (TEAD2) (NM_001256660) Human Tagged ORF Clone
CAT＃: RG232761
- TrueORF®
TEAD2 (tGFP-tagged) - Homo sapiens TEA domain family member 2 (TEAD2), transcript variant 3
ORF Plasmid: DDK
"NM_001256660" in other vectors (2)
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CNY 4,370.00
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Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | ETF; TEAD-2; TEF-4; TEF4 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG232761 representing NM_001256660
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGGAACCCCGGGCTGGGGCCGCCCTGGACGATGGCAGCGGCTGGACGGGCAGTGAGGAAGGCAGTG AGGAGGGTACCGGCGGCAGTGAGGGGGCTGGGGGTGACGGGGGCCCGGATGCAGAGGGGGTGTGGAGCCC AGACATTGAGCAGAGCTTCCAGGAGGCCCTGGCCATCTATCCACCCTGCGGCCGCCGGAAAATAATTTTG TCTGATGAAGGCAAGATGTATGGTCGGAATGAACTGATCGCCCGCTACATCAAGCTGAGAACGGGGAAGA CCCGAACTCGAAAACAGGTTTCTAGTCACATCCAGGTTTTGGCCCGAAGGAAATCAAGGGAAATCCAGTC CAAGTTGAAGGCTCTGAACGTGGACCAGGTTTCCAAGGACAAGGCTTTCCAGACAATGGCAACCATGTCC TCTGCCCAGCTCATCTCCGCGCCTTCTCTGCAGGCCAAACTGGGTCCCACTGGTCCTCAGGCCTCTGAGC TTTTCCAGTTTTGGTCTGGAGGATCTGGGCCCCCCTGGAATGTTCCAGATGTGAAGCCATTCTCACAGAC ACCGTTCACCTTGTCACTGACTCCCCCATCTACTGACCTCCCAGGGTACGAGCCCCCCCAAGCCCTCTCA CCCCTGCCCCCACCTACCCCATCGCCCCCAGCCTGGCAGGCTCGGGGCCTGGGCACCGCCCGGTTGCAGC TGGTAGAGTTCTCAGCCTTCGTGGAACCGCCAGATGCAGTTGATTCTTACCAGAGGCACCTGTTCGTGCA CATCAGCCAGCACTGCCCCAGCCCCGGAGCGCCGCCGCTCGAGAGTGTGGACGTCCGGCAGATCTACGAC AAATTCCCTGAGAAAAAGGGTGGCCTCCGAGAGCTATATGATCGTGGCCCCCCCCATGCCTTCTTCCTGG TCAAGTTCTGGGCGGACCTGAACTGGGGCCCAAGTGGTGAGGAGGCAGGGGCCGGTGGCAGCATCAGCAG TGGTGGCTTCTACGGAGTGAGCAGCCAGTATGAGAGCCTGGAACACATGACCCTCACCTGTTCCTCCAAG GTCTGCTCTTTTGGCAAGCAGGTGGTGGAGAAGGTGGAGACGGAACGGGCCCAGCTGGAGGACGGCAGAT TTGTGTACCGCCTGCTGCGCTCGCCCATGTGCGAGTACCTGGTGAATTTCTTGCACAAGTTGCGGCAGCT GCCTGAGCGATACATGATGAACAGCGTCCTGGAAAACTTCACCATCCTCCAGGTGGTGACAAACAGAGAC ACCCAGGAACTGCTGCTCTGCACCGCCTATGTCTTCGAGGTCTCCACCAGCGAGCGTGGGGCCCAGCATC ACATTTACCGCCTGGTCAGGGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG232761 representing NM_001256660
Red=Cloning site Green=Tags(s) MGEPRAGAALDDGSGWTGSEEGSEEGTGGSEGAGGDGGPDAEGVWSPDIEQSFQEALAIYPPCGRRKIIL SDEGKMYGRNELIARYIKLRTGKTRTRKQVSSHIQVLARRKSREIQSKLKALNVDQVSKDKAFQTMATMS SAQLISAPSLQAKLGPTGPQASELFQFWSGGSGPPWNVPDVKPFSQTPFTLSLTPPSTDLPGYEPPQALS PLPPPTPSPPAWQARGLGTARLQLVEFSAFVEPPDAVDSYQRHLFVHISQHCPSPGAPPLESVDVRQIYD KFPEKKGGLRELYDRGPPHAFFLVKFWADLNWGPSGEEAGAGGSISSGGFYGVSSQYESLEHMTLTCSSK VCSFGKQVVEKVETERAQLEDGRFVYRLLRSPMCEYLVNFLHKLRQLPERYMMNSVLENFTILQVVTNRD TQELLLCTAYVFEVSTSERGAQHHIYRLVRD TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001256660 |
| ORF Size | 1353 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001256660.2 |
| RefSeq Size | 2236 bp |
| RefSeq ORF | 1356 bp |
| Locus ID | 8463 |
| UniProt ID | Q15562 |
| Protein Families | Transcription Factors |
| Gene Summary | Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds to the SPH and GT-IIC 'enhansons' (5'-GTGGAATGT-3'). May be involved in the gene regulation of neural development. Binds to the M-CAT motif.[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
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