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PGM3 (NM_001199917) Human Tagged ORF Clone
CAT＃: RG232979
- TrueORF®
PGM3 (tGFP-tagged) - Homo sapiens phosphoglucomutase 3 (PGM3), transcript variant 1
ORF Plasmid: DDK
"NM_001199917" in other vectors (2)
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CNY 6,270.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AGM1; IMD23; PAGM; PGM 3 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG232979 representing NM_001199917
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAGGAGAGTGGGGACAATCTGCTATCTGCCCTGAGTCTGCCCAGGAGTGGACATACCAAGTTGGAC AACATCTTGTAGACATGGATTTAGGTGCTATTACAAAATACTCAGCATTACACGCCAAGCCCAATGGACT GATCCTTCAATACGGGACTGCTGGATTTCGAACGAAGGCAGAACATCTTGATCATGTCATGTTTCGCATG GGATTATTAGCTGTCCTGAGGTCAAAACAGACAAAATCCACTATAGGAGTCATGGTAACAGCGTCCCACA ATCCTGAGGAAGACAATGGTGTAAAATTGGTTGATCCTTTGGGTGAAATGTTGGCACCATCCTGGGAGGA ACATGCCACCTGTTTAGCAAATGCTGAGGAACAAGATATGCAGAGAGTGCTTATTGACATCAGCGAGAAA GAAGCTGTGAATCTGCAACAAGATGCCTTTGTAGTTATTGGTAGAGATACCAGGCCCAGCAGTGAGAAAC TTTCACAATCTGTAATAGATGGTGTGACTGTTCTAGGAGGTCAATTCCATGATTATGGCTTGTTAACAAC ACCCCAGCTGCACTACATGGTGTATTGTCGAAACACGGGTGGCCGATATGGAAAGGCAACTATAGAAGGT TACTACCAGAAACTCTCTAAGGCTTTTGTGGAACTCACCAAACAGGCTTCTTGCAGTGGAGATGAATACA GATCACTTAAGGTTGACTGTGCAAATGGCATAGGGGCCCTGAAGCTAAGGGAAATGGAACACTACTTCTC ACAGGGCCTGTCAGTTCAGCTGTTTAATGATGGGTCCAAGGGCAAACTCAATCATTTATGTGGAGCTGAC TTTGTGAAAAGTCATCAGAAACCTCCACAGGGAATGGAAATTAAGTCCAATGAAAGATGCTGTTCTTTTG ATGGAGATGCAGACAGAATTGTTTATTACTACCATGATGCAGATGGCCACTTTCATCTCATAGATGGAGA CAAGATAGCAACGTTAATTAGCAGTTTCCTTAAAGAGCTCCTGGTGGAGATTGGAGAAAGTTTGAATATT GGTGTTGTACAAACTGCATATGCAAATGGAAGTTCAACACGGTATCTTGAAGAAGTTATGAAGGTACCTG TCTATTGCACTAAGACTGGTGTAAAACATTTGCACCACAAGGCTCAAGAGTTTGACATTGGAGTTTATTT TGAAGCAAATGGGCATGGCACTGCACTGTTTAGTACAGCTGTTGAAATGAAGATAAAACAATCAGCAGAA CAACTGGAAGATAAGAAAAGAAAAGCTGCTAAGATGCTTGAAAACATTATTGACTTGTTTAACCAGGCAG CTGGTGATGCTATTTCTGACATGCTGGTGATTGAAGCAATCTTGGCTCTGAAGGGCTTGACTGTACAACA GTGGGATGCTCTCTATACAGATCTTCCAAACAGACAACTTAAAGTTCAGGTTGCAGACAGGAGAGTTATT AGCACTACCGATGCTGAAAGACAAGCAGTTACACCCCCAGGATTACAGGAGGCAATCAATGACCTGGTGA AGAAGTACAAGCTTTCTCGAGCTTTTGTCCGGCCCTCTGGTACAGAAGATGTCGTCCGAGTATATGCAGA AGCAGACTCACAAGAAAGTGCAGATCACCTTGCACATGAAGTGAGCTTGGCAGTATTTCAGCTGGCTGGA GGAATTGGAGAAAGGCCCCAACCAGGTTTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG232979 representing NM_001199917
Red=Cloning site Green=Tags(s) MGGEWGQSAICPESAQEWTYQVGQHLVDMDLGAITKYSALHAKPNGLILQYGTAGFRTKAEHLDHVMFRM GLLAVLRSKQTKSTIGVMVTASHNPEEDNGVKLVDPLGEMLAPSWEEHATCLANAEEQDMQRVLIDISEK EAVNLQQDAFVVIGRDTRPSSEKLSQSVIDGVTVLGGQFHDYGLLTTPQLHYMVYCRNTGGRYGKATIEG YYQKLSKAFVELTKQASCSGDEYRSLKVDCANGIGALKLREMEHYFSQGLSVQLFNDGSKGKLNHLCGAD FVKSHQKPPQGMEIKSNERCCSFDGDADRIVYYYHDADGHFHLIDGDKIATLISSFLKELLVEIGESLNI GVVQTAYANGSSTRYLEEVMKVPVYCTKTGVKHLHHKAQEFDIGVYFEANGHGTALFSTAVEMKIKQSAE QLEDKKRKAAKMLENIIDLFNQAAGDAISDMLVIEAILALKGLTVQQWDALYTDLPNRQLKVQVADRRVI STTDAERQAVTPPGLQEAINDLVKKYKLSRAFVRPSGTEDVVRVYAEADSQESADHLAHEVSLAVFQLAG GIGERPQPGF TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001199917 |
| ORF Size | 1710 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001199917.2 |
| RefSeq Size | 6243 bp |
| RefSeq ORF | 1713 bp |
| Locus ID | 5238 |
| UniProt ID | O95394 |
| Protein Pathways | Amino sugar and nucleotide sugar metabolism |
| Gene Summary | This gene encodes a member of the phosphohexose mutase family. The encoded protein mediates both glycogen formation and utilization by catalyzing the interconversion of glucose-1-phosphate and glucose-6-phosphate. A non-synonymous single nucleotide polymorphism in this gene may play a role in resistance to diabetic nephropathy and neuropathy. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2010] |
Documents
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