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Cellular Apoptosis Susceptibility (CSE1L) (NM_001256135) Human Tagged ORF Clone
CAT＃: RG233172
- TrueORF®
CSE1L (tGFP-tagged) - Homo sapiens CSE1 chromosome segregation 1-like (yeast) (CSE1L), transcript variant 2
ORF Plasmid: DDK
"NM_001256135" in other vectors (2)
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CNY 7,890.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CAS; CSE1; XPO2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG233172 representing NM_001256135
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAACTCAGCGATGCAAATCTGCAAACACTAACAGAATATTTAAAGAAAACACTTGATCCTGATCCTG CCATCCGACGTCCAGCTGAGAAATTTCTTGAATCTGTTGAAGGAAATCAGAATTATCCACTGTTGCTTTT GACATTACTGGAGAAGTCCCAGGATAATGTTATCAAAGTATGTGCTTCAGTAACATTCAAAAACTATATT AAAAGGAACTGGAGAATTGTTGAAGATGAACCAAACAAAATTTGTGAAGCCGATCGAGTGGCCATTAAAG CCAACATAGTGCACTTGATGCTTAGCAGCCCAGAGCAAATTCAGAAGCAGTTAAGTGATGCAATTAGCAT TATTGGCAGAGAAGATTTTCCACAGAAATGGCCTGACTTGCTGACAGAAATGGTGAATCGCTTTCAGAGT GGAGATTTCCATGTTATTAATGGAGTCCTCCGTACAGCACATTCATTATTTAAAAGATACCGTCATGAAT TTAAGTCAAACGAGTTATGGACTGAAATTAAGCTTGTTCTGGATGCCTTTGCTTTGCCTTTGACTAATCT TTTTAAGGCCACTATTGAACTCTGCAGTACCCATGCAAATGATGCCTCTGCCCTGAGGATTCTGTTTTCT TCCCTGATCCTGATCTCAAAATTGTTCTATAGTTTAAACTTTCAGGATCTCCCTGAATTTTTTGAAGATA ATATGGAAACTTGGATGAATAATTTTCATACTCTCTTAACATTGGATAATAAGCTTTTACAAACTGATTT GGTAAGTAATGCAATTCAATTTCTGGCTTCAGTTTGTGAGAGACCTCATTATAAGAATCTATTTGAGGAC CAGAACACGCTGACAAGTATCTGTGAAAAGGTTATTGTGCCTAACATGGAATTTAGAGCTGCTGATGAAG AAGCATTTGAAGATAATTCTGAGGAGTACATAAGGAGAGATTTGGAAGGATCTGATATTGATACTAGACG CAGGGCTGCTTGTGATCTGGTACGAGGATTATGCAAGTTTTTTGAGGGACCTGTGACAGGAATCTTCTCT GGTTATGTTAATTCCATGCTGCAGGAATACGCAAAAAATCCATCTGTCAACTGGAAACACAAAGATGCAG CCATCTACCTAGTGACATCTTTGGCATCAAAAGCCCAAACACAGAAGCATGGAATTACACAAGCAAATGA ACTTGTAAACCTAACTGAGTTCTTTGTGAATCACATCCTCCCTGATTTAAAATCAGCTAATGTGAATGAA TTTCCTGTCCTTAAAGCTGACGGTATCAAATATATTATGATTTTTAGAAATCAAGTGCCAAAAGAACATC TTTTAGTCTCGATTCCTCTCTTGATTAATCATCTTCAAGCTGAAAGTATTGTTGTTCATACTTACGCAGC TCATGCTCTTGAACGGCTCTTTACTATGCGAGGGCCTAACAATGCCACTCTCTTTACAGCTGCAGAAATC GCACCGTTTGTTGAGATTCTGCTAACAAACCTTTTCAAAGCTCTCACACTTCCTGGCTCTTCAGAAAATG AATATATTATGAAAGCTATCATGAGAAGTTTTTCTCTCCTACAAGAAGCCATAATCCCCTACATCCCTAC TCTCATCACTCAGCTTACACAGAAGCTATTAGCTGTTAGTAAGAACCCAAGCAAACCTCACTTTAATCAC TACATGTTTGAAGCAATATGTTTATCCATAAGAATAACTTGCAAAGCTAACCCTGCTGCTGTTGTAAATT TTGAGGAGGCTTTGTTTTTGGTGTTTACTGAAATCTTACAAAATGATGTGCAAGAATTTATTCCATACGT CTTTCAAGTGATGTCTTTGCTTCTGGAAACACACAAAAATGACATCCCGTCTTCCTATATGGCCTTATTT CCTCATCTCCTTCAGCCAGTGCTTTGGGAAAGAACAGGAAATATTCCTGCTCTAGTGAGGCTTCTTCAAG CATTCTTAGAACGCGGTTCAAACACAATAGCAAGTGCTGCAGCTGACAAAATTCCTGGGTTACTAGGTGT CTTTCAGAAGCTGATTGCATCCAAAGCAAATGACCACCAAGGTTTTTATCTTCTAAACAGTATAATAGAG CACATGCCTCCTGAATCAGTTGACCAATATAGGAAACAAATCTTCATTCTGCTATTCCAGAGACTTCAGA ATTCCAAAACAACCAAGTTTATCAAGAGTTTTTTAGTCTTTATTAATTTGTATTGCATAAAATATGGGGC ACTAGCACTACAAGAAATATTTGATGGTATACAACCAAAAATGTTTGGAATGGTTTTGGAAAAAATTATT ATTCCTGAAATTCAGAAGGTATCTGGAAATGTAGAGAAAAAGATCTGTGCGGTTGGCATAACCAAATTAC TAACAGAATGTCCCCCAATGATGGACACTGAGTATACCAAACTGTGGACTCCATTATTACAGTCTTTGAT TGGTCTTTTTGAGTTACCCGAAGATGATACCATTCCTGATGAGGAACATTTTATTGACATAGAAGATACA CCAGGATATCAGACTGCCTTCTCACAGTTGGCATTTGCTGGGAAAAAAGAGCATGATCCTGTAGGTCAAA TGGTGAATAACCCCAAAATTCACCTGGCACAGTCACTTCACAAGTTGTCTACCGCCTGTCCAGGAAGGGT TCCATCAATGGTGAGCACCAGCCTGAATGCAGAAGCGCTCCAGTATCTCCAAGGGTACCTTCAGGCAGCC AGTGTGACACTGCTT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG233172 representing NM_001256135
Red=Cloning site Green=Tags(s) MELSDANLQTLTEYLKKTLDPDPAIRRPAEKFLESVEGNQNYPLLLLTLLEKSQDNVIKVCASVTFKNYI KRNWRIVEDEPNKICEADRVAIKANIVHLMLSSPEQIQKQLSDAISIIGREDFPQKWPDLLTEMVNRFQS GDFHVINGVLRTAHSLFKRYRHEFKSNELWTEIKLVLDAFALPLTNLFKATIELCSTHANDASALRILFS SLILISKLFYSLNFQDLPEFFEDNMETWMNNFHTLLTLDNKLLQTDLVSNAIQFLASVCERPHYKNLFED QNTLTSICEKVIVPNMEFRAADEEAFEDNSEEYIRRDLEGSDIDTRRRAACDLVRGLCKFFEGPVTGIFS GYVNSMLQEYAKNPSVNWKHKDAAIYLVTSLASKAQTQKHGITQANELVNLTEFFVNHILPDLKSANVNE FPVLKADGIKYIMIFRNQVPKEHLLVSIPLLINHLQAESIVVHTYAAHALERLFTMRGPNNATLFTAAEI APFVEILLTNLFKALTLPGSSENEYIMKAIMRSFSLLQEAIIPYIPTLITQLTQKLLAVSKNPSKPHFNH YMFEAICLSIRITCKANPAAVVNFEEALFLVFTEILQNDVQEFIPYVFQVMSLLLETHKNDIPSSYMALF PHLLQPVLWERTGNIPALVRLLQAFLERGSNTIASAAADKIPGLLGVFQKLIASKANDHQGFYLLNSIIE HMPPESVDQYRKQIFILLFQRLQNSKTTKFIKSFLVFINLYCIKYGALALQEIFDGIQPKMFGMVLEKII IPEIQKVSGNVEKKICAVGITKLLTECPPMMDTEYTKLWTPLLQSLIGLFELPEDDTIPDEEHFIDIEDT PGYQTAFSQLAFAGKKEHDPVGQMVNNPKIHLAQSLHKLSTACPGRVPSMVSTSLNAEALQYLQGYLQAA SVTLL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001256135 |
| ORF Size | 2745 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001256135.1, NP_001243064.1 |
| RefSeq Size | 3459 bp |
| RefSeq ORF | 2748 bp |
| Locus ID | 1434 |
| UniProt ID | P55060 |
| Protein Families | Druggable Genome |
| Gene Summary | Proteins that carry a nuclear localization signal (NLS) are transported into the nucleus by the importin-alpha/beta heterodimer. Importin-alpha binds the NLS, while importin-beta mediates translocation through the nuclear pore complex. After translocation, RanGTP binds importin-beta and displaces importin-alpha. Importin-alpha must then be returned to the cytoplasm, leaving the NLS protein behind. The protein encoded by this gene binds strongly to NLS-free importin-alpha, and this binding is released in the cytoplasm by the combined action of RANBP1 and RANGAP1. In addition, the encoded protein may play a role both in apoptosis and in cell proliferation. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012] |
Documents
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| SDS |
Resources
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