C5orf19 (REEP2) (NM_001271803) Human Tagged ORF Clone
CAT#: RG233562
- TrueORF®
REEP2 (tGFP-tagged) - Homo sapiens receptor accessory protein 2 (REEP2), transcript variant 1
ORF Plasmid: DDK
"NM_001271803" in other vectors (2)
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CNY 4,370.00
CNY 1,000.00
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | C5orf19; SGC32445; SPG72; Yip2d |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG233562 representing NM_001271803
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGTCCTGGATCATCTCTCGCCTGGTGGTGCTCATCTTTGGCACCCTGTACCCAGCCTATTCTTCCT ACAAGGCCGTGAAGACAAAAAACGTGAAGGAATATGTGAAATGGATGATGTACTGGATCGTCTTTGCCTT CTTCACCACGGCCGAGACGCTCACGGATATAGTGCTCTCCTGGTTCCCCTTCTACTTTGAACTGAAGATC GCCTTCGTGATATGGCTGCTGTCCCCTTACACCAAGGGCTCCAGCGTGCTCTACCGCAAGTTCGTGCACC CAACGCTGTCCAACAAGGAGAAGGAGATCGACGAGTACATCACGCAGGCCCGAGACAAGAGCTATGAGAC CATGATGAGGGTGGGCAAGAGGGGCCTGAACCTTGCCGCCAATGCTGCAGTCACAGCTGCCGCCAAGGGC CAGGGGGTGCTGTCAGAGAAGCTCCGCAGCTTCAGCATGCAGGACCTGACCCTGATCCGGGACGAGGACG CACTGCCCCTGCAGAGGCCTGACGGCCGCCTCCGACCCAGCCCTGGCAGCCTCCTGGACACCATCGAGGA CTTAGGAGATGACCCTGCCCTGAGTCTAAGGTCCAGCACAAACCCGGCAGATTCCCGGACAGAGGCTTCT GAGGATGACATGGGAGACAAAGCTCCCAAGAGGGCCAAACCCATCAAAAAAGCGCCCAAAGCTGAGCCAC TGGCTTCCAAGACACTGAAGACCCGGCCCAAGAAGAAGACCTCTGGCGGGGGCGACTCAGCT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG233562 representing NM_001271803
Red=Cloning site Green=Tags(s) MVSWIISRLVVLIFGTLYPAYSSYKAVKTKNVKEYVKWMMYWIVFAFFTTAETLTDIVLSWFPFYFELKI AFVIWLLSPYTKGSSVLYRKFVHPTLSNKEKEIDEYITQARDKSYETMMRVGKRGLNLAANAAVTAAAKG QGVLSEKLRSFSMQDLTLIRDEDALPLQRPDGRLRPSPGSLLDTIEDLGDDPALSLRSSTNPADSRTEAS EDDMGDKAPKRAKPIKKAPKAEPLASKTLKTRPKKKTSGGGDSA TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001271803 |
ORF Size | 762 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_001271803.2 |
RefSeq Size | 2209 bp |
RefSeq ORF | 765 bp |
Locus ID | 51308 |
UniProt ID | Q9BRK0 |
Protein Families | Druggable Genome, Transmembrane |
Gene Summary | This gene encodes a member of the receptor expression enhancing protein family. Studies of a related gene in mouse suggest that the encoded protein is found in the cell membrane and enhances the function of sweet taste receptors. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2012] |
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