CD2BP2 (NM_001243646) Human Tagged ORF Clone
CAT#: RG233819
- TrueORF®
CD2BP2 (tGFP-tagged) - Homo sapiens CD2 (cytoplasmic tail) binding protein 2 (CD2BP2), transcript variant 2
ORF Plasmid: DDK
"NM_001243646" in other vectors (2)
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CNY 4,370.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | FWP010; LIN1; PPP1R59; Snu40; U5-52K |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG233819 representing NM_001243646
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCAAAGAGGAAAGTGACCTTCCAAGGCGTGGGAGATGAGGAGGATGAGGATGAAATCATTGTCCCCA AGAAGAAGCTGGTGGACCCTGTGGCTGGGTCAGGGGGTCCTGGGAGCCGCTTTAAAGGCAAACACTCTTT GGATAGCGATGAGGAGGAGGATGATGATGATGGGGGGTCCAGCAAATATGACATCTTGGCCTCAGAGGAT GTAGAAGGTCAGGAGGCAGCCACACTCCCCAGCGAGGGGGGTGTTCGGATCACACCCTTTAACCTGCAGG AGGAGATGGAGGAAGGCCACTTTGATGCCGATGGCAACTACTTCCTGAACCGGGATGCTCAGATCCGAGA CAGCTGGCTGGACAACATTGACTGGGTGAAGATCCGGGAGCGGCCACCTGGCCAGCGCCAGGCCTCAGAC TCGGAGGAGGAGGACAGCTTGGGCCAGACCTCAATGAGTGCCCAAGCCCTCTTGGAGGGACTTTTGGAGC TCCTATTGCCTAGAGAGACAGTGGCTGGGGCACTGAGGCGTCTGGGGGCCCGAGGAGGAGGCAAAGGGAG AAAGGGGCCTGGGCAACCCAGTTCCCCTCAGCGCCTGGACCGGCTCTCCGGGTTGGCCGACCAGATGGTG GCCCGGGGCAACCTTGGTGTGTACCAGGAAACAAGGGAACGGTTGGCTATGCGTCTGAAGGGTTTGGGGT GTCAGACCCTAGGACCCCACAATCCCACACCCCCACCCTCCCTGGACATGTTCGCTGAGGAGTTGGCGGA GGAGGAACTGGAGACCCCAACCCCTACCCAGAGAGGAGAAGCAGAGTCGCGGGGAGATGGTCTGGTGGAT GTGATGTGGGAATATAAGTGGGAGAACACGGGGGATGCCGAGCTGTATGGGCCCTTCACCAGCGCCCAGA TGCAGACCTGGGTGAGTGAAGGCTACTTCCCGGACGGTGTTTATTGCCGGAAGCTGGACCCCCCTGGTGG TCAGTTCTACAACTCCAAACGCATTGACTTTGACCTCTACACC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG233819 representing NM_001243646
Red=Cloning site Green=Tags(s) MPKRKVTFQGVGDEEDEDEIIVPKKKLVDPVAGSGGPGSRFKGKHSLDSDEEEDDDDGGSSKYDILASED VEGQEAATLPSEGGVRITPFNLQEEMEEGHFDADGNYFLNRDAQIRDSWLDNIDWVKIRERPPGQRQASD SEEEDSLGQTSMSAQALLEGLLELLLPRETVAGALRRLGARGGGKGRKGPGQPSSPQRLDRLSGLADQMV ARGNLGVYQETRERLAMRLKGLGCQTLGPHNPTPPPSLDMFAEELAEEELETPTPTQRGEAESRGDGLVD VMWEYKWENTGDAELYGPFTSAQMQTWVSEGYFPDGVYCRKLDPPGGQFYNSKRIDFDLYT TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001243646 |
ORF Size | 1023 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001243646.1, NP_001230575.1 |
RefSeq Size | 3513 bp |
RefSeq ORF | 1026 bp |
Locus ID | 10421 |
UniProt ID | O95400 |
Gene Summary | This gene encodes a bi-functional protein. In the cytoplasm, the encoded protein binds the cytoplasmic tail of human surface antigen CD2 via its C-terminal GYF domain, and regulate CD2-triggered T lymphocyte activation. In the nucleus, this protein is a component of the U5 small nuclear ribonucleoprotein complex and is involved in RNA splicing. A pseudogene has been identified on chromosome 7. Alternative splicing results in multiple transcript variants but their biological validity has not been determined. [provided by RefSeq, Nov 2008] |
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