SETMAR (NM_001276325) Human Tagged ORF Clone
CAT#: RG233886
- TrueORF®
SETMAR (tGFP-tagged) - Homo sapiens SET domain and mariner transposase fusion gene (SETMAR), transcript variant 4
ORF Plasmid: DDK
"NM_001276325" in other vectors (2)
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CNY 4,370.00
CNY 1,000.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | Mar1; METNASE |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG233886 representing NM_001276325
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCGCGGAAGCGGCAAAGACGACACGGCCTTGTGGGATGGCGGAGTTTAAGGAGAAGCCTGAGGCCC CGACTGAGCAGCTGGATGTCGCGTGCGGCCAGGAAAACTTGCCGGTGGGCGCGTGGCCCCCGGGGGCCGC GCCGGCGCCCTTCCAGTACACTCCTGATCATGTAGTTGGACCTGGAGCAGACATTGATCCCACTCAAATA ACCTTTCCCGGATGCATTTGTGTCAAAACTCCCTGCCTCCCTGGCACTTGCTCCTGTCTCCGCCATGGAG AGAACTATGATGATAACTCATGCCTTAGAGATATAGGATCTGGAGGAAAGTATGCAGAGCCTGTTTTTGA ATGCAATGTCCTGTGCCGATGCAGTGACCACTGCAGAAACAGAGTGGTCCAGAAAGGTCTACAGTTCCAC TTCCAAGTGTTCAAGACGCATAAAAAAGGCTGGGGACTTCGTACCTTGGAATTTATACCGAAAGGAAGGT TTGTCTGTGAATATGCTGGTGAGGTTTTAGGATTCTCTGAAGTTCAGAGAAGAATTCACTTACAAACAAA ATCCGACTCCAATTACATTATAGCCATCAGGGAACATGTTTATAATGGGCAGGTAATGGAAACATTTGTT GACCCTACTTATATAGGAAATATTGGAAGATTCCTTAATCATTCTTGTGAGCCAAACCTTTTGATGATTC CTGTCCGAATTGACTCAATGGTACCTAAGTTGGCACTTTTTGCAGCCAAAGATATTGTGCCAGAAGAAGA ACTCTCTTATGATTATTCAGGAAGATATCTTAATCTAACAGTCAGTGAAGACAAAGAAAGGCTAGATCAT GGGAAACTAAGGAAACCTTGTTACTGTGGTGCCAAATCATGTACTGCTTTCCTGCCTTTTGACAGTTCTC TGTACTGCCCCGTAGAAAAGTCGAACATCAGTTGTGGAAATGAGAAGGAACCCAGCATGTGTGGCTCAGC CCCTTCTGTGTTCCCCTCCTGCAAGCGATTGACCCTTGAGGTGAGTCTGTTCAGTGATAAGCAGCTTGCC CCTCCCTATAGTGGAAGACAGTGGTTGGCTAGCTTTACCTCTGCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG233886 representing NM_001276325
Red=Cloning site Green=Tags(s) MFAEAAKTTRPCGMAEFKEKPEAPTEQLDVACGQENLPVGAWPPGAAPAPFQYTPDHVVGPGADIDPTQI TFPGCICVKTPCLPGTCSCLRHGENYDDNSCLRDIGSGGKYAEPVFECNVLCRCSDHCRNRVVQKGLQFH FQVFKTHKKGWGLRTLEFIPKGRFVCEYAGEVLGFSEVQRRIHLQTKSDSNYIIAIREHVYNGQVMETFV DPTYIGNIGRFLNHSCEPNLLMIPVRIDSMVPKLALFAAKDIVPEEELSYDYSGRYLNLTVSEDKERLDH GKLRKPCYCGAKSCTAFLPFDSSLYCPVEKSNISCGNEKEPSMCGSAPSVFPSCKRLTLEVSLFSDKQLA PPYSGRQWLASFTSA TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001276325 |
ORF Size | 1095 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001276325.1, NP_001263254.1 |
RefSeq Size | 1775 bp |
RefSeq ORF | 1098 bp |
Locus ID | 6419 |
UniProt ID | Q53H47 |
Protein Families | Druggable Genome, Transcription Factors |
Protein Pathways | Lysine degradation |
Gene Summary | This gene encodes a fusion protein that contains an N-terminal histone-lysine N-methyltransferase domain and a C-terminal mariner transposase domain. The encoded protein binds DNA and functions in DNA repair activities including non-homologous end joining and double strand break repair. The SET domain portion of this protein specifically methylates histone H3 lysines 4 and 36. This gene exists as a fusion gene only in anthropoid primates, other organisms lack mariner transposase domain. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013] |
Documents
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FAQs |
SDS |