PFKFB1 (NM_001271805) Human Tagged ORF Clone
CAT#: RG234007
- TrueORF®
PFKFB1 (tGFP-tagged) - Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (PFKFB1), transcript variant 3
ORF Plasmid: DDK
"NM_001271805" in other vectors (2)
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CNY 4,370.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | F6PK; HL2K; PFRX |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG234007 representing NM_001271805
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGAAAAAACCTCTAGAATAAAAGTGTTTAATTTAGGCCAGTATCGACGAGAGGCAGTGAGCTACA AGAACTATGAATTCTTTCTTCCAGACAACATGGAAGCCCTGCAAATCAGGAAGCAGTGCGCCCTGGCAGC CCTGAAGGATGTTCACAACTATCTCAGCCATGAGGAAGGTCATGTTGCGGTTTTTGATGCCACCAACACT ACCAGAGAACGACGGTCACTGATCCTGCAGTTTGCAAAAGAACATGGTTACAAGGTGTTTTTCATTGAGT CCATTTGTAATGACCCTGGCATAATTGCAGAAAACATCAGGCAAGTGAAACTTGGCAGCCCTGATTATAT AGACTGTGACCGGGAAAAGGTTCTGGAAGACTTTCTAAAGAGAATTGAGTGCTATGAGGTCAACTACCAA CCCTTGGATGAGGAACTGGACAGCCACCTGTCCTACATCAAGATCTTCGACGTGGGCACACGCTACATGG TGAACCGAGTGCAGGATCACATCCAGAGCCGCACAGTCTACTACCTCATGAATATCCATGTCACACCTCG CTCCATCTACCTTTGCCGACATGGCGAGAGTGAACTCAACATCAGAGGCCGCATCGGAGGTGACTCTGGC CTCTCAGTTCGCGGCAAGCAGTATGCCTATGCCCTGGCCAACTTCATTCAGTCCCAGGGCATCAGCTCCC TGAAGGTGTGGACCAGTCACATGAAGAGGACCATCCAGACAGCTGAGGCCCTGGGTGTCCCCTATGAGCA GTGGAAGGCCCTGAATGAGATTGATGCGGGTGTCTGTGAGGAGATGACCTATGAAGAAATCCAGGAACAT TACCCTGAAGAATTTGCACTGCGAGACCAAGATAAATATCGCTACCGCTATCCCAAGGGAGAGTCCTATG AGGATCTGGTTCAGCGTCTGGAGCCAGTGATAATGGAGCTAGAACGACAGGAGAATGTACTGGTGATCTG CCACCAGGCTGTCATGCGGTGCCTCCTGGCCTATTTCCTGGATAAAAGTTCAGATGAGCTTCCATATCTC AAGTGCCCTCTGCACACAGTGCTCAAACTCACTCCTGTGGCTTATGGCTGCAAAGTGGAATCCATCTACC TGAATGTGGAGGCCGTGAACACACACCGGGAGAAGCCTGAGAATGTGGACATCACCCGGGAACCTGAGGA AGCCCTGGATACTGTCCCAGCCCACTAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG234007 representing NM_001271805
Red=Cloning site Green=Tags(s) MEEKTSRIKVFNLGQYRREAVSYKNYEFFLPDNMEALQIRKQCALAALKDVHNYLSHEEGHVAVFDATNT TRERRSLILQFAKEHGYKVFFIESICNDPGIIAENIRQVKLGSPDYIDCDREKVLEDFLKRIECYEVNYQ PLDEELDSHLSYIKIFDVGTRYMVNRVQDHIQSRTVYYLMNIHVTPRSIYLCRHGESELNIRGRIGGDSG LSVRGKQYAYALANFIQSQGISSLKVWTSHMKRTIQTAEALGVPYEQWKALNEIDAGVCEEMTYEEIQEH YPEEFALRDQDKYRYRYPKGESYEDLVQRLEPVIMELERQENVLVICHQAVMRCLLAYFLDKSSDELPYL KCPLHTVLKLTPVAYGCKVESIYLNVEAVNTHREKPENVDITREPEEALDTVPAHY TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001271805 |
ORF Size | 1218 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001271805.1, NP_001258734.1 |
RefSeq Size | 1591 bp |
RefSeq ORF | 1221 bp |
Locus ID | 5207 |
UniProt ID | P16118 |
Protein Families | Druggable Genome |
Protein Pathways | Fructose and mannose metabolism |
Gene Summary | This gene encodes a member of the family of bifunctional 6-phosphofructo-2-kinase:fructose-2,6-biphosphatase enzymes. The enzyme forms a homodimer that catalyzes both the synthesis and degradation of fructose-2,6-biphosphate using independent catalytic domains. Fructose-2,6-biphosphate is an activator of the glycolysis pathway and an inhibitor of the gluconeogenesis pathway. Consequently, regulating fructose-2,6-biphosphate levels through the activity of this enzyme is thought to regulate glucose homeostasis. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Nov 2012] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC234007 | PFKFB1 (Myc-DDK tagged) - Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (PFKFB1), transcript variant 3 |
CNY 3,990.00 |
|
SC333119 | PFKFB1 (untagged) - Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (PFKFB1), transcript variant 3 |
CNY 3,900.00 |