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RGS6 (NM_001204422) Human Tagged ORF Clone
CAT＃: RG234073
- TrueORF®
RGS6 (tGFP-tagged) - Homo sapiens regulator of G-protein signaling 6 (RGS6), transcript variant 8
ORF Plasmid: DDK
"NM_001204422" in other vectors (2)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | GAP; HA117; S914 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG234073 representing NM_001204422
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCAAGGATCCGGGGATCAAAGAGCAGTGGGGGTTGCTGACCCAGAGGAGAGTTCTCCAAACATGA TCGTTTACTGCAAAATTGAAGACATCATTACAAAGATGCAAGATGACAAGACAGGGGGTGTGCCCATCAG AACAGTCAAGAGCTTTCTCTCCAAAATCCCCAGTGTCGTCACAGGTACTGACATTGTGCAGTGGCTTATG AAGAACCTTTCCATTGAGGACCCAGTTGAAGCAATACACTTGGGGAGCCTTATCGCTGCCCAGGGCTACA TCTTTCCAATCTCAGACCATGTTCTCACCATGAAGGATGATGGCACCTTTTATCGTTTCCAGGCTCCGTA CTTCTGGCCTTCGAACTGCTGGGAACCTGAAAACACTGACTATGCCATCTATCTCTGTAAGAGGACAATG CAAAATAAAGCAAGGCTGGAACTGGCAGATTATGAAGCAGAAAACTTAGCAAGACTCCAGAGGGCCTTTG CGAGGAAGTGGGAATTCATCTTTATGCAAGCAGAAGCACAAGTAAAGATTGACCGGAAAAAAGACAAGAC AGAAAGGAAAATTTTGGATAGTCAAGAACGAGCCTTTTGGGATGTCCACAGGCCTGTGCCAGGCTGTGTG AACACAACAGAAATGGATATCCGAAAATGTCGACGTTTGAAGAATCCACAAAAGGTTAAAAAGTCCGTGT ATGGCGTGACTGAAGAGTCCCAGGCACAGAGCCCGGTGCATGTACTCAGCCAACCAATCAGGAAAACAAC AAAAGAGGACATCCGGAAACAGATAACATTTTTGAACGCACAGATCGACAGACATTGTTTGAAAATGTCC AAAGTGGCTGAAAGCAAAGAGCCCAGCCAACAGCGAGTAAAAAGATGGGGCTTCTCTTTCGATGAGATAT TGAAGGACCAGGTGGGGCGGGACCAGTTTCTACGATTCCTGGAGTCCGAATTCAGTTCAGAAAACCTCAG GTTCTGGCTGGCTGTCCAAGATCTTAAGAAACAACCCCTACAGGATGTGGCCAAGAGGGTAGAAGAAATC TGGCAAGAGTTTCTGGCTCCAGGGGCTCCAAGTGCAATCAACCTGGATTCTCACAGCTATGAGATAACCA GTCAAAATGTCAAAGATGGAGGGAGATATACATTTGAAGACGCCCAGGAGCACATCTACAAGCTGATGAA GAGTGACAGCTATGCCCGCTTCCTCCGGTCAAATGCTTACCAGGATTTGCTGCTGGCCAAGAAGAAGTTG TATTCCAACACTCCACTCGCTAAGAGGCCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG234073 representing NM_001204422
Red=Cloning site Green=Tags(s) MAQGSGDQRAVGVADPEESSPNMIVYCKIEDIITKMQDDKTGGVPIRTVKSFLSKIPSVVTGTDIVQWLM KNLSIEDPVEAIHLGSLIAAQGYIFPISDHVLTMKDDGTFYRFQAPYFWPSNCWEPENTDYAIYLCKRTM QNKARLELADYEAENLARLQRAFARKWEFIFMQAEAQVKIDRKKDKTERKILDSQERAFWDVHRPVPGCV NTTEMDIRKCRRLKNPQKVKKSVYGVTEESQAQSPVHVLSQPIRKTTKEDIRKQITFLNAQIDRHCLKMS KVAESKEPSQQRVKRWGFSFDEILKDQVGRDQFLRFLESEFSSENLRFWLAVQDLKKQPLQDVAKRVEEI WQEFLAPGAPSAINLDSHSYEITSQNVKDGGRYTFEDAQEHIYKLMKSDSYARFLRSNAYQDLLLAKKKL YSNTPLAKRP TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001204422 |
| ORF Size | 1290 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001204422.3 |
| RefSeq Size | 1551 bp |
| RefSeq ORF | 1293 bp |
| Locus ID | 9628 |
| UniProt ID | P49758 |
| Protein Families | Druggable Genome |
| Gene Summary | This gene encodes a member of the RGS (regulator of G protein signaling) family of proteins, which are defined by the presence of a RGS domain that confers the GTPase-activating activity of these proteins toward certain G alpha subunits. This protein also belongs to a subfamily of RGS proteins characterized by the presence of DEP and GGL domains, the latter a G beta 5-interacting domain. The RGS proteins negatively regulate G protein signaling, and may modulate neuronal, cardiovascular, lymphocytic activities, and cancer risk. Many alternatively spliced transcript variants encoding different isoforms with long or short N-terminal domains, complete or incomplete GGL domains, and distinct C-terminal domains, have been described for this gene, however, the full-length nature of some of these variants is not known.[provided by RefSeq, Mar 2011] |
Documents
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