HTF9C (TRMT2A) (NM_001257994) Human Tagged ORF Clone
CAT#: RG234441
- TrueORF®
TRMT2A (tGFP-tagged) - Homo sapiens tRNA methyltransferase 2 homolog A (S. cerevisiae) (TRMT2A), transcript variant 3
ORF Plasmid: DDK
"NM_001257994" in other vectors (2)
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CNY 5,040.00
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | HTF9C |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG234441 representing NM_001257994
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGAGAACCTCGACAACGAGGGCCCGAAGCCCATGGAGAGCTGTGGCCAGGAGAGCAGCAGTGCCC TGAGCTGCCCTACCGTCTCGGTGCCCCCTGCAGCCCCGGCAGCCCTGGAGGAGGTGGAGAAAGAGGGCGC TGGGGCGGCTACAGGGCCGGGGCCTCAGCCCGGGCTCTACAGCTACATCAGGGATGACTTGTTTACCTCT GAGATCTTTAAACTGGAGCTGCAGAACGTGCCTCGCCACGCCAGCTTCAGCGACGTCCGGCGCTTCCTGG GCCGCTTTGGTCTGCAGCCCCACAAAACCAAACTCTTTGGGCAACCACCCTGCGCCTTTGTGACATTCCG CAGCGCTGCAGAGAGGGACAAGGCCCTGCGCGTTTTGCATGGTGCCCTCTGGAAAGGCCGCCCACTCAGT GTGCGCCTGGCCCGGCCCAAGGCCGACCCCATGGCCAGGAGGAGGCGACAGGAGGGTGAGAGTGAGCCAC CAGTAACACGAGTGGCCGACGTGGTGACCCCTCTATGGACAGTGCCCTATGCTGAGCAGCTTGAGCGGAA GCAGCTGGAGTGCGAGCAGGTGCTGCAGAAACTTGCCAAGGAAATCGGGAGCACCAACCGTGCCTTGCTG CCCTGGCTGCTCGAGCAGAGGCACAAGCACAACAAGGCCTGCTGCCCGCTGGAGGGGGTCAGGCCATCAC CCCAGCAGACTGAGTATCGTAATAAGTGTGAGTTTCTGGTTGGCGTCGGGGTGGATGGGGAGGATAACAC CGTGGGCTGTCGGCTCGGCAAGTACAAGGGCGGGACGTGTGCTGTGGCAGCCCCGTTTGACACCGTGCAC ATCCCCGAAGCCACCAAGCAGGTGGTGAAGGCCTTCCAGGAGTTCATCCGGTCCACTCCATACTCGGCAT ACGACCCAGAGACGTACACAGGCCACTGGAAGCAGCTGACTGTGCGCACCAGCCGCCGCCACCAGGCCAT GGCCATTGCCTACTTCCACCCCCAGAAGCTGAGCCCTGAGGAGCTGGCAGAGCTGAAGACCTCCCTAGCG CAGCACTTCACAGCAGGGCCAGGCAGGGCCAGTGGAGTGACCTGCCTCTACTTCGTGGAGGAGGGACAGC GAAAGACTCCTAGCCAGGAGGGCCTGCCCCTGGAGCATGTGGCTGGGGACCGGTGCATCCACGAGGACCT GCTAGGGCTGACCTTCCGGATCTCTCCACACGCCTTCTTCCAGGTGAACACACCCGCAGCCGAGGTGCTC TACACAGTCATCCAGGACTGGGCCCAATTGGATGCGGGGAGCATGGTGCTGGACGTGTGCTGTGGCACCG GCACCATTGGCCTGGCCCTGGCCCGGAAGGTAAAGAGGGTCATTGGGGTCGAGCTATGCCCAGAGGCTGT GGAGGACGCCCGGGTGAACGCCCAGGACAATGAGTTGAGTAATGTGGAGTTCCACTGCGGGAGGGCCGAG GACCTGGTGCCCACCCTGGTGAGCAGACTGGCCTCCCAGCACCTCGTGGCCATCCTGGACCCACCCCGTG CTGGCTTGCATTCCAAGGTGATCCTGGCCATCCGGAGAGCTAAGAACCTCAGGCGGCTGCTGTACGTCTC ATGCAACCCCCGGGCAGCCATGGGCAACTTTGTGGACGCCCCCCTTTTCCCCCCACAGCCTCTGCAGAGC CCCATC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG234441 representing NM_001257994
Red=Cloning site Green=Tags(s) MSENLDNEGPKPMESCGQESSSALSCPTVSVPPAAPAALEEVEKEGAGAATGPGPQPGLYSYIRDDLFTS EIFKLELQNVPRHASFSDVRRFLGRFGLQPHKTKLFGQPPCAFVTFRSAAERDKALRVLHGALWKGRPLS VRLARPKADPMARRRRQEGESEPPVTRVADVVTPLWTVPYAEQLERKQLECEQVLQKLAKEIGSTNRALL PWLLEQRHKHNKACCPLEGVRPSPQQTEYRNKCEFLVGVGVDGEDNTVGCRLGKYKGGTCAVAAPFDTVH IPEATKQVVKAFQEFIRSTPYSAYDPETYTGHWKQLTVRTSRRHQAMAIAYFHPQKLSPEELAELKTSLA QHFTAGPGRASGVTCLYFVEEGQRKTPSQEGLPLEHVAGDRCIHEDLLGLTFRISPHAFFQVNTPAAEVL YTVIQDWAQLDAGSMVLDVCCGTGTIGLALARKVKRVIGVELCPEAVEDARVNAQDNELSNVEFHCGRAE DLVPTLVSRLASQHLVAILDPPRAGLHSKVILAIRRAKNLRRLLYVSCNPRAAMGNFVDAPLFPPQPLQS PI TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001257994 |
ORF Size | 1686 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001257994.2 |
RefSeq Size | 3003 bp |
RefSeq ORF | 1689 bp |
Locus ID | 27037 |
UniProt ID | Q8IZ69 |
Protein Families | Druggable Genome |
Gene Summary | The protein encoded by this gene is of unknown function. However, it is orthologous to the mouse Trmt2a gene and contains an RNA methyltransferase domain. Expression of this gene varies during the cell cycle, with aberrant expression being a possible biomarker in certain breast cancers. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Apr 2012] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC234441 | TRMT2A (Myc-DDK tagged) - Homo sapiens tRNA methyltransferase 2 homolog A (S. cerevisiae) (TRMT2A), transcript variant 3 |
CNY 4,560.00 |
|
SC332558 | TRMT2A (untagged) - Homo sapiens tRNA methyltransferase 2 homolog A (S. cerevisiae) (TRMT2A), transcript variant 3 |
CNY 5,510.00 |