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AP180 (SNAP91) (NM_001242794) Human Tagged ORF Clone
CAT＃: RG234526
- TrueORF®
SNAP91 (tGFP-tagged) - Homo sapiens synaptosomal-associated protein, 91kDa (SNAP91), transcript variant 5
ORF Plasmid: DDK
"NM_001242794" in other vectors (2)
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CNY 5,040.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AP180; CALM |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG234526 representing NM_001242794
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGGCCAAACGCTCACGGATCGGATCGCCGCCGCTCAGTACAGCGTTACAGGCTCTGCTGTAGCAA GAGCGGTCTGCAAAGCCACTACTCATGAAGTAATGGGCCCCAAGAAAAAGCACCTGGACTATTTGATCCA GGCTACCAACGAGACCAATGTTAATATTCCTCAGATGGCCGACACTCTCTTTGAGCGGGCAACAAACAGT AGCTGGGTGGTTGTGTTTAAGGCTTTAGTGACAACACATCATCTCATGGTGCATGGAAATGAGAGATTTA TTCAATATTTGGCTTCTAGAAATACACTATTCAATCTCAGCAATTTTTTGGACAAAAGTGGATCCCATGG TTATGATATGTCTACCTTCATAAGGCGCTATAGTAGATATTTGAATGAAAAGGCTTTTTCTTACAGACAG ATGGCCTTTGATTTTGCCAGGGTGAAGAAAGGGGCCGATGGTGTAATGAGGACAATGGCTCCCGAAAAGC TGCTAAAGAGTATGCCAATACTACAGGGACAAATTGATGCACTGCTTGAATTTGATGTGCATCCAAATGA ACTAACAAATGGTGTCATAAATGCAGCATTTATGCTTCTTTTCAAAGATCTTATCAAACTTTTTGCTTGC TACAATGATGGTGTTATTAACTTACTCGAAAAGTTTTTTGAAATGAAGAAAGGACAATGTAAAGATGCTC TAGAAATTTACAAACGATTTCTAACTAGAATGACACGAGTGTCTGAATTTCTCAAGGTTGCAGAGGCTCC CAGCAGTCTTATGGAGACGCTTGAACAGCATCTAAATACATTAGAAGGAAAGAAACCTGGAAACAAATCT GGTGCTCCCTCTCCATTAAGTAAGTCTTCTCCAGCCACAACTGTTACGTCTCCTAATTCTACACCAGCTA AAACTATTGACACATCCCCACCGGTTGATTTATTTGCAACTGCATCTGCGGCTGTCCCAGTCAGCACTTC TAAACCATCTAGTGATCTCCTGGACCTCCAGCCAGACTTTTCCTCTGGAGGGGCAGCAGCAGCCGCAGCA CCAGCACCACCACCACCTGCTGGAGGAGCCACTGCATGGGGAGGATTTGGGGGTTCTTTCATGGCGCCTT CCCCATCTCCAGTGACTCCAGCTCAGAATAACCTGCTACAGCCCAATTTTGAGGCAGCTTTTGGGACAAC GCCTTCAACTTCCAGCAGCAGCTCCTTTGATCCATCAGTGTTTGATGGTCTAGGTGATCTTTTGATGCCA ACCATGGCACCAGCTGGGCAGCCTGCACCTGTCTCAATGGTACCACCCAGTCCTGCAATGGCAGCCAGCA AAGCCCTTGGAAGTGATCTTGATTCATCTCTTGCCAGCTTAGTAGGCAATCTTGGAATTTCTGGTACCAC AACAAAAAAGGGAGATCTTCAGTGGAATGCTGGAGAGAAAAAGTTGACTGGTGGAGCCAACTGGCAGCCT AAAGTAGCTCCAGCAACCTGGTCAGCAGGCGTTCCACCAAGTGCACCTTTGCAAGGAGCTGTACCTCCAA CCAGTTCAGTTCCTCCTGTTGCCGGGGCCCCATCGGTTGGACAACCTGGAGCAGGATTTGGAATGCCTCC TGCTGGGACAGGCATGCCCATGATGCCTCAGCAGCCGGTCATGTTTGCACAGCCCATGATGAGGCCCCCC TTTGGAGCTGCCGCTGTACCTGGCACGCAGCTTTCTCCAAGCCCTACACCTGCCAGTCAGAGTCCCAAGA AACCTCCAGCAAAGGACCCATTAGCGGATCTTAACATCAAGGATTTCTTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG234526 representing NM_001242794
Red=Cloning site Green=Tags(s) MSGQTLTDRIAAAQYSVTGSAVARAVCKATTHEVMGPKKKHLDYLIQATNETNVNIPQMADTLFERATNS SWVVVFKALVTTHHLMVHGNERFIQYLASRNTLFNLSNFLDKSGSHGYDMSTFIRRYSRYLNEKAFSYRQ MAFDFARVKKGADGVMRTMAPEKLLKSMPILQGQIDALLEFDVHPNELTNGVINAAFMLLFKDLIKLFAC YNDGVINLLEKFFEMKKGQCKDALEIYKRFLTRMTRVSEFLKVAEAPSSLMETLEQHLNTLEGKKPGNKS GAPSPLSKSSPATTVTSPNSTPAKTIDTSPPVDLFATASAAVPVSTSKPSSDLLDLQPDFSSGGAAAAAA PAPPPPAGGATAWGGFGGSFMAPSPSPVTPAQNNLLQPNFEAAFGTTPSTSSSSSFDPSVFDGLGDLLMP TMAPAGQPAPVSMVPPSPAMAASKALGSDLDSSLASLVGNLGISGTTTKKGDLQWNAGEKKLTGGANWQP KVAPATWSAGVPPSAPLQGAVPPTSSVPPVAGAPSVGQPGAGFGMPPAGTGMPMMPQQPVMFAQPMMRPP FGAAAVPGTQLSPSPTPASQSPKKPPAKDPLADLNIKDFL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001242794 |
| ORF Size | 1800 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001242794.1, NP_001229723.1 |
| RefSeq Size | 3340 bp |
| RefSeq ORF | 1803 bp |
| Locus ID | 9892 |
| UniProt ID | O60641 |
| Gene Summary | Adaptins are components of the adapter complexes which link clathrin to receptors in coated vesicles. Clathrin-associated protein complexes are believed to interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration. Binding of AP180 to clathrin triskelia induces their assembly into 60-70 nm coats (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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