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KIFAP3 (NM_001204514) Human Tagged ORF Clone
CAT＃: RG234761
- TrueORF®
KIFAP3 (tGFP-tagged) - Homo sapiens kinesin-associated protein 3 (KIFAP3), transcript variant 2
ORF Plasmid: DDK
"NM_001204514" in other vectors (2)
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CNY 6,370.00
货期*
5周
规格
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经常一起买 (5)
热销推荐Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | dJ190I16.1; FLA3; KAP-1; KAP-3; KAP3; SMAP; Smg-GDS |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG234761 representing NM_001204514
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGATTGCCCTTGGCAACACTTACCGGAGAAGTCTTTCAGATTCAGAAACTGTTTCTGTACTCCAAG CAAGCTTATGTGAAGAGAAAAAAGAAAAATCAAGCAAGCCTAAAGATCCACCTCCTTTTGAAGGAATGGA GATTGATGAAGTTGCTAACATTAATGACATGGATGAATATATTGAGTTATTATATGAAGATATTCCTGAC AAAGTTCGGGGTTCTGCTTTGATCCTGCAGCTTGCTCGAAATCCTGATAACTTGGAAGAACTACTATTGA ATGAAACTGCCCTTGGTGCATTAGCAAGGGTCCTGAGAGAAGACTGGAAGCAAAGTGTCGAGTTAGCTAC AAACATAATTTACATCTTTTTTTGTTTCTCCAGCTTTTCTCAATTTCATGGACTTATTACTCACTATAAA ATTGGAGCTCTGTGTATGAATATTATTGATCATGAGTTAAAAAGACATGAGCTTTGGCAAGAAGAACTCT CAAAGAAGAAGAAAGCTGTTGATGAAGACCCTGAAAACCAAACCTTGAGAAAGGATTATGAAAAAACCTT TAAAAAGTACCAGGGGCTTGTGGTAAAACAGGAACAGCTATTACGAGTTGCTCTTTATTTGCTTCTGAAT CTTGCTGAGGATACTCGTACCGAACTGAAAATGAGGAACAAGAACATAGTTCACATGTTGGTGAAAGCCC TTGATCGGGACAATTTTGAGCTGCTAATTTTAGTTGTGTCATTCTTGAAGAAACTCAGCATTTTTATGGA GAATAAAAATGATATGGTGGAAATGGATATTGTTGAAAAACTGGTGAAAATGATACCTTGTGAGCATGAA GACCTGCTGAATATCACCCTCCGACTTTTACTAAACCTATCCTTTGACACAGGACTGAGGAATAAGATGG TACAAGTTGGACTGCTTCCCAAGCTCACTGCACTCCTAGGCAATGACAACTACAAACAAATAGCAATGTG TGTTCTTTACCACATAAGCATGGATGACCGCTTTAAATCAATGTTTGCATACACTGACTGTATACCACAG TTAATGAAGATGCTGTTTGAATGTTCAGATGAACGAATTGACTTGGAACTCATTTCTTTCTGCATTAATC TTGCTGCTAACAAAAGAAATGTACAGCTTATCTGTGAAGGAAATGGGCTGAAGATGCTCATGAAGAGGGC TCTGAAGTTTAAGGATCCATTGCTGATGAAAATGATTAGAAACATTTCTCAGCATGATGGACCAACTAAA AATCTGTTTATTGATTATGTTGGGGACCTTGCAGCCCAGATCTCTAATGATGAAGAAGAGGAGTTTGTGA TTGAATGTTTGGGAACTCTTGCAAACTTGACCATTCCAGACTTAGACTGGGAATTGGTTCTTAAAGAATA TAAGTTGGTTCCATACCTCAAGGATAAACTAAAACCAGGTGCTGCAGAAGATGATCTTGTTTTAGAAGTG GTTATAATGATTGGAACTGTATCCATGGATGACTCTTGTGCTGCATTGCTAGCCAAATCTGGCATAATCC CTGCACTCATTGAATTGCTAAATGCTCAACAAGAAGATGATGAATTTGTGTGTCAGATAATTTATGTCTT CTACCAGATGGTTTTCCACCAAGCCACAAGAGACGTCATAATCAAGGAAACACAGGCTCCAGCATATCTC ATAGACCTAATGCATGATAAGAATAATGAAATCCGAAAGGTCTGTGATAATACATTAGATATTATAGCGG AATATGATGAAGAATGGGCTAAGAAAATTCAGAGTGAAAAGTTTCGCTGGCATAACTCTCAGTGGCTGGA GATGGTAGAGAGTCGTCAGATGGATGAGAGTGAGCAGTACTTGTATGGTGATGATCGAATTGAGCCATAC ATTCATGAAGGAGATATTCTCGAAAGACCTGACCTTTTCTACAACTCAGATGGATTAATTGCCTCTGAAG GAGCCATAAGTCCCGATTTCTTCAATGATTACCACCTTCAAAATGGAGATGTTGTTGGGCAGCATTCATT TCCTGGCAGCCTTGGAATGGATGGCTTTGGCCAACCAGTTGGCATTCTTGGACGCCCTGCCACAGCATAT GGATTCCGCCCTGATGAACCTTACTACTATGGCTATGGATCT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG234761 representing NM_001204514
Red=Cloning site Green=Tags(s) MSIALGNTYRRSLSDSETVSVLQASLCEEKKEKSSKPKDPPPFEGMEIDEVANINDMDEYIELLYEDIPD KVRGSALILQLARNPDNLEELLLNETALGALARVLREDWKQSVELATNIIYIFFCFSSFSQFHGLITHYK IGALCMNIIDHELKRHELWQEELSKKKKAVDEDPENQTLRKDYEKTFKKYQGLVVKQEQLLRVALYLLLN LAEDTRTELKMRNKNIVHMLVKALDRDNFELLILVVSFLKKLSIFMENKNDMVEMDIVEKLVKMIPCEHE DLLNITLRLLLNLSFDTGLRNKMVQVGLLPKLTALLGNDNYKQIAMCVLYHISMDDRFKSMFAYTDCIPQ LMKMLFECSDERIDLELISFCINLAANKRNVQLICEGNGLKMLMKRALKFKDPLLMKMIRNISQHDGPTK NLFIDYVGDLAAQISNDEEEEFVIECLGTLANLTIPDLDWELVLKEYKLVPYLKDKLKPGAAEDDLVLEV VIMIGTVSMDDSCAALLAKSGIIPALIELLNAQQEDDEFVCQIIYVFYQMVFHQATRDVIIKETQAPAYL IDLMHDKNNEIRKVCDNTLDIIAEYDEEWAKKIQSEKFRWHNSQWLEMVESRQMDESEQYLYGDDRIEPY IHEGDILERPDLFYNSDGLIASEGAISPDFFNDYHLQNGDVVGQHSFPGSLGMDGFGQPVGILGRPATAY GFRPDEPYYYGYGS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001204514 |
| ORF Size | 2142 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001204514.1, NP_001191443.1 |
| RefSeq Size | 3175 bp |
| RefSeq ORF | 2145 bp |
| Locus ID | 22920 |
| UniProt ID | Q92845 |
| Protein Families | Druggable Genome |
| Gene Summary | The small G protein GDP dissociation stimulator (smg GDS) is a regulator protein having two activities on a group of small G proteins including the Rho and Rap1 family members and Ki-Ras; one is to stimulate their GDP/GTP exchange reactions, and the other is to inhibit their interactions with membranes. The protein encoded by this gene contains 9 'Armadillo' repeats and interacts with the smg GDS protein through these repeats. This protein, which is highly concentrated around the endoplasmic reticulum, is phosphorylated by v-src, and this phosphorylation reduces the affinity of the protein for smg GDS. It is thought that this protein serves as a linker between human chromosome-associated polypeptide (HCAP) and KIF3A/B, a kinesin superfamily protein in the nucleus, and that it plays a role in the interaction of chromosomes with an ATPase motor protein. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2011] |
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