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ARHGAP22 (NM_001256024) Human Tagged ORF Clone
CAT＃: RG234762
- TrueORF®
ARHGAP22 (tGFP-tagged) - Homo sapiens Rho GTPase activating protein 22 (ARHGAP22), transcript variant 1
ORF Plasmid: DDK
"NM_001256024" in other vectors (2)
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CNY 6,370.00
货期*
5周
规格
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经常一起买 (5)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | RhoGAP2; RhoGap22 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG234762 representing NM_001256024
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGAGCCCAAAGATCAGGCAGGCCAGGAGGGCCCGCTCCAAAAGCCTAGTGATGGGGGAGCAGAGCC GGAGCCCTGGGCGGATGCCGTGCCCTCACAGGCTGGGCCCCGTGCTGAAGGCGGGCTGGCTGAAGAAGCA GAGGAGCATCATGAAGAACTGGCAGCAGCGCTGGTTTGTGCTGCGTGGGGATCAGCTTTTCTACTACAAG GACAAAGATGAGATCAAGCCCCAGGGATTTATTTCTCTACAAGGGACACAGGTGACTGAACTTCCTCCTG GCCCCGAGGACCCAGGGAAGCACCTCTTTGAGATCAGCCCAGGTGGTGCCGGGGAGCGGGAGAAGGTGCC GGCCAACCCCGAGGCGCTCCTGCTCATGGCCAGCTCCCAGCGTGACATGGAGGACTGGGTGCAGGCCATC CGCCGAGTCATCTGGGCCCCGCTGGGCGGAGGTACTGCCCGTAGATCGCATGCCCACCCTCTAGAACCCT TGCCTCCAGGGATCTTTGGGCAGCGCCTAGAGGAAACAGTCCACCACGAGCGGAAGTATGGCCCCCGCCT GGCGCCCCTGCTGGTGGAGCAGTGTGTGGACTTCATCCGGGAGCGCGGGCTCACTGAGGAGGGGCTGTTC CGCATGCCAGGCCAGGCCAACCTGGTGAGGGACCTGCAGGATTCCTTCGACTGTGGGGAGAAGCCACTGT TTGACAGCACAACAGACGTGCACACGGTGGCCTCCCTGCTGAAGCTGTACCTGCGGGAGCTCCCCGAGCC CGTGGTCCCCTTCGCCAGGTACGAGGACTTCCTCAGCTGCGCCCAGCTGCTCACCAAGGACGAGGGGGAG GGCACTCTGGAGTTGGCTAAACAAGTGAGCAACCTTCCTCAGGCAAATTACAACCTGCTCAGATACATCT GCAAGTTTCTGGATGAAGTTCAGGCATACTCAAATGTCAACAAGATGAGTGTCCAGAATCTGGCAACCGT TTTTGGACCTAACATTCTGCGGCCACAGGTAGAGGACCCAGTAACCATCATGGAAGGCACTTCCCTCGTC CAGCACCTGATGACCGTCCTCATCCGCAAACACAGCCAGCTCTTCACGGCACCGGTCCCGGAAGGGCCCA CCTCCCCGCGCGGGGGCCTGCAATGCGCAGTGGGGTGGGGCTCCGAGGAGGTCACCAGGGACAGCCAAGG AGAGCCCGGCGGCCCCGGCCTGCCCGCGCACAGGACCTCTTCCCTGGACGGGGCGGCCGTGGCGGTGCTC TCCAGAACAGCCCCCACGGGGCCGGGGAGCCGGTGCAGCCCTGGGAAGAAGGTGCAGACCCTGCCCAGTT GGAAGTCCTCCTTCCGGCAGCCGAGGTCCCTATCGGGAAGCCCGAAGGGGGGCGGCTCATCCCTGGAGGT GCCCATCATCTCCTCCGGCGGGAACTGGCTTATGAACGGGCTGTCCTCCCTGCGCGGACACCGCCGGGCC TCGTCGGGAGACCGGCTCAAGGACTCGGGCTCCGTGCAGAGACTCTCCACCTACGACAATGTGCCCGCGC CGGGCCTGGTCCCCGGCATACCCAGCGTGGCCAGTATGGCGTGGTCCGGGGCCTCGTCCAGCGAGTCGTC GGTGGGGGGCTCACTCAGCAGCTGCACGGCCTGCCGCGCCAGCGACTCGTCTGCCCGCAGTTCCCTGCAC ACCGACTGGGCCCTGGAGCCCTCCCCGCTCCCCAGCAGCAGCGAGGACCCCAAGTCCCTGGACCTGGACC ACAGCATGGACGAGGCGGGCGCGGGTGCCAGCAACAGCGAGCCCAGCGAGCCGGACAGCCCCACCCGGGA ACACGCGCGCCGCTCCGAGGCCTTACAGGGGCTGGTCACTGAGCTCAGGGCCGAGCTGTGCCGCCAGCGG ACTGAGTACGAGAGGAGTGTGAAAAGAATCGAAGAAGGGAGTGCTGACCTGAGAAAACGAATGTCCCGGT TAGAAGAAGAACTGGACCAGGAAAAGAAAAAATACATCATGCTGGAAATAAAGCTGCGGAACTCTGAACG GGCGCGGGAGGATGCGGAGAGGAGGAACCAGCTGTTGCAGAGGGAAATGGAGGAGTTTTTTTCGACCCTA GGAAGCTTGACTGTTGGGGCAAAAGGTGCCAGGGCCCCAAAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG234762 representing NM_001256024
Red=Cloning site Green=Tags(s) MLSPKIRQARRARSKSLVMGEQSRSPGRMPCPHRLGPVLKAGWLKKQRSIMKNWQQRWFVLRGDQLFYYK DKDEIKPQGFISLQGTQVTELPPGPEDPGKHLFEISPGGAGEREKVPANPEALLLMASSQRDMEDWVQAI RRVIWAPLGGGTARRSHAHPLEPLPPGIFGQRLEETVHHERKYGPRLAPLLVEQCVDFIRERGLTEEGLF RMPGQANLVRDLQDSFDCGEKPLFDSTTDVHTVASLLKLYLRELPEPVVPFARYEDFLSCAQLLTKDEGE GTLELAKQVSNLPQANYNLLRYICKFLDEVQAYSNVNKMSVQNLATVFGPNILRPQVEDPVTIMEGTSLV QHLMTVLIRKHSQLFTAPVPEGPTSPRGGLQCAVGWGSEEVTRDSQGEPGGPGLPAHRTSSLDGAAVAVL SRTAPTGPGSRCSPGKKVQTLPSWKSSFRQPRSLSGSPKGGGSSLEVPIISSGGNWLMNGLSSLRGHRRA SSGDRLKDSGSVQRLSTYDNVPAPGLVPGIPSVASMAWSGASSSESSVGGSLSSCTACRASDSSARSSLH TDWALEPSPLPSSSEDPKSLDLDHSMDEAGAGASNSEPSEPDSPTREHARRSEALQGLVTELRAELCRQR TEYERSVKRIEEGSADLRKRMSRLEEELDQEKKKYIMLEIKLRNSERAREDAERRNQLLQREMEEFFSTL GSLTVGAKGARAPK TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001256024 |
| ORF Size | 2142 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001256024.2 |
| RefSeq Size | 2846 bp |
| RefSeq ORF | 2145 bp |
| Locus ID | 58504 |
| UniProt ID | Q7Z5H3 |
| Gene Summary | This gene encodes a member of the GTPase activating protein family which activates a GTPase belonging to the RAS superfamily of small GTP-binding proteins. The encoded protein is insulin-responsive, is dependent on the kinase Akt and requires the Akt-dependent 14-3-3 binding protein which binds sequentially to two serine residues. The result of these interactions is regulation of cell motility. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011] |
Documents
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