United States
Germany
Japan
United Kingdom
China
ECT2 (NM_001258315) Human Tagged ORF Clone
CAT＃: RG235028
- TrueORF®
ECT2 (tGFP-tagged) - Homo sapiens epithelial cell transforming sequence 2 oncogene (ECT2), transcript variant 1
ORF Plasmid: DDK
"NM_001258315" in other vectors (2)
Need custom modification / cloning service?
Get a free quote
CNY 7,890.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | ARHGEF31 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG235028 representing NM_001258315
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGAAAATAGTGTATTAACATCCACTACTGGGAGGACTAGCTTGGCAGACTCTTCCATTTTTGATT CTAAAGTTACTGAGATTTCCAAGGAAAACTTACTTATTGGATCTACTTCATATGTAGAAGAAGAGATGCC TCAGATTGAAACAAGAGTGATATTGGTTCAAGAAGCTGGAAAACAAGAAGAACTTATAAAAGCCTTAAAG ACTATTAAAATAATGGAAGTCCCTGTTATAAAGATAAAAGAAAGTTGTCCTGGAAAATCGGATGAAAAAT TAATAAAAAGTGTTATTAATATGGACATTAAAGTGGGCTTTGTAAAGATGGAGTCAGTGGAAGAATTTGA AGGTTTGGATTCTCCGGAATTTGAAAATGTATTTGTAGTCACGGACTTTCAGGATTCTGTCTTTAATGAC CTCTACAAGGCTGATTGTAGAGTTATTGGACCACCAGTTGTATTAAATTGTTCACAAAAAGGAGAGCCTT TGCCATTTTCATGTCGCCCGTTGTATTGTACAAGTATGATGAATCTAGTACTATGCTTTACTGGATTTAG GAAAAAAGAAGAACTAGTCAGGTTGGTGACATTGGTCCATCACATGGGTGGAGTTATTCGAAAAGACTTT AATTCAAAAGTTACACATTTGGTGGCAAATTGTACACAAGGAGAAAAATTCAGGGTTGCTGTGAGTCTAG GTACTCCAATTATGAAGCCAGAATGGATTTATAAAGCTTGGGAAAGGCGGAATGAACAGGATTTCTATGC AGCAGTTGATGACTTTAGAAATGAATTTAAAGTTCCTCCATTTCAAGATTGTATTTTAAGTTTCCTGGGA TTTTCAGATGAAGAGAAAACCAATATGGAAGAAATGACTGAAATGCAAGGAGGTAAATATTTACCGCTTG GAGATGAAAGATGCACTCACCTTGTAGTTGAAGAGAATATAGTAAAAGATCTTCCCTTTGAACCTTCAAA GAAACTTTATGTTGTCAAGCAAGAGTGGTTCTGGGGAAGCATTCAAATGGATGCCCGAGCTGGAGAAACT ATGTATTTATATGAAAAGGCAAATACTCCTGAGCTCAAGAAATCAGTGTCAATGCTTTCTCTAAATACCC CTAACAGCAATCGCAAACGACGTCGTTTAAAAGAAACACTTGCTCAGCTTTCAAGAGAGACAGACGTGTC ACCATTTCCACCCCGTAAGCGCCCATCAGCTGAGCATTCCCTTTCCATAGGGTCACTCCTAGATATCTCC AACACACCAGAGTCTAGCATTAACTATGGAGACACCCCAAAGTCTTGTACTAAGTCTTCTAAAAGCTCCA CTCCAGTTCCTTCAAAGCAGTCAGCAAGGTGGCAAGTTGCAAAAGAGCTTTATCAAACTGAAAGTAATTA TGTTAATATATTGGCAACAATTATTCAGTTATTTCAAGTACCATTGGAAGAGGAAGGACAACGTGGTGGA CCTATCCTTGCACCAGAGGAGATTAAGACTATTTTTGGTAGCATCCCAGATATCTTTGATGTACACACTA AGATAAAGGATGATCTTGAAGACCTTATAGTTAATTGGGATGAGAGCAAAAGCATTGGTGACATTTTTCT GAAATATTCAAAAGATTTGGTAAAAACCTACCCTCCCTTTGTAAACTTCTTTGAAATGAGCAAGGAAACA ATTATTAAATGTGAAAAACAGAAACCAAGATTTCATGCTTTTCTCAAGATAAACCAAGCAAAACCAGAAT GTGGACGGCAGAGCCTTGTTGAACTTCTTATCCGACCAGTACAGAGGTTACCCAGTGTTGCATTACTTTT AAATGATCTTAAGAAGCATACAGCTGATGAAAATCCAGACAAAAGCACTTTAGAAAAAGCTATTGGATCA CTGAAGGAAGTAATGACGCATATTAATGAGGATAAGAGAAAAACAGAAGCTCAAAAGCAAATTTTTGATG TTGTTTATGAAGTAGATGGATGCCCAGCTAATCTTTTATCTTCTCACCGAAGCTTAGTACAGCGGGTTGA AACAATTTCTCTAGGTGAGCACCCCTGTGACAGAGGAGAACAAGTAACTCTCTTCCTCTTCAATGATTGC CTAGAGATAGCAAGAAAACGGCACAAGGTTATTGGCACTTTTAGGAGTCCTCATGGCCAAACCCGACCCC CAGCTTCTCTTAAGCATATTCACCTAATGCCTCTTTCTCAGATTAAGAAGGTATTGGACATAAGAGAGAC AGAAGATTGCCATAATGCTTTTGCCTTGCTTGTGAGGCCACCAACAGAGCAGGCAAATGTGCTACTCAGT TTCCAGATGACATCAGATGAACTTCCAAAAGAAAACTGGCTAAAGATGCTGTGTCGACATGTAGCTAACA CCATTTGTAAAGCAGATGCTGAGAATCTTATTTATACTGCTGATCCAGAATCCTTTGAAGTAAATACAAA AGATATGGACAGTACATTGAGTAGAGCATCAAGAGCAATAAAAAAGACTTCAAAAAAGGTTACAAGAGCA TTCTCTTTCTCCAAAACTCCAAAAAGAGCTCTTCGAAGGGCTCTTATGACATCCCACGGCTCAGTGGAGG GAAGAAGTCCTTCCAGCAATGATAAGCATGTAATGAGTCGTCTTTCTAGCACATCATCATTAGCAGGTAT CCCTTCTCCCTCCCTTGTCAGCCTTCCTTCCTTCTTTGAAAGGAGAAGTCATACGTTAAGTAGATCTACA ACTCATTTGATA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG235028 representing NM_001258315
Red=Cloning site Green=Tags(s) MAENSVLTSTTGRTSLADSSIFDSKVTEISKENLLIGSTSYVEEEMPQIETRVILVQEAGKQEELIKALK TIKIMEVPVIKIKESCPGKSDEKLIKSVINMDIKVGFVKMESVEEFEGLDSPEFENVFVVTDFQDSVFND LYKADCRVIGPPVVLNCSQKGEPLPFSCRPLYCTSMMNLVLCFTGFRKKEELVRLVTLVHHMGGVIRKDF NSKVTHLVANCTQGEKFRVAVSLGTPIMKPEWIYKAWERRNEQDFYAAVDDFRNEFKVPPFQDCILSFLG FSDEEKTNMEEMTEMQGGKYLPLGDERCTHLVVEENIVKDLPFEPSKKLYVVKQEWFWGSIQMDARAGET MYLYEKANTPELKKSVSMLSLNTPNSNRKRRRLKETLAQLSRETDVSPFPPRKRPSAEHSLSIGSLLDIS NTPESSINYGDTPKSCTKSSKSSTPVPSKQSARWQVAKELYQTESNYVNILATIIQLFQVPLEEEGQRGG PILAPEEIKTIFGSIPDIFDVHTKIKDDLEDLIVNWDESKSIGDIFLKYSKDLVKTYPPFVNFFEMSKET IIKCEKQKPRFHAFLKINQAKPECGRQSLVELLIRPVQRLPSVALLLNDLKKHTADENPDKSTLEKAIGS LKEVMTHINEDKRKTEAQKQIFDVVYEVDGCPANLLSSHRSLVQRVETISLGEHPCDRGEQVTLFLFNDC LEIARKRHKVIGTFRSPHGQTRPPASLKHIHLMPLSQIKKVLDIRETEDCHNAFALLVRPPTEQANVLLS FQMTSDELPKENWLKMLCRHVANTICKADAENLIYTADPESFEVNTKDMDSTLSRASRAIKKTSKKVTRA FSFSKTPKRALRRALMTSHGSVEGRSPSSNDKHVMSRLSSTSSLAGIPSPSLVSLPSFFERRSHTLSRST THLI TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001258315 |
| ORF Size | 2742 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001258315.2 |
| RefSeq Size | 4192 bp |
| RefSeq ORF | 2745 bp |
| Locus ID | 1894 |
| UniProt ID | Q9H8V3 |
| Protein Families | Druggable Genome |
| Gene Summary | The protein encoded by this gene is a guanine nucleotide exchange factor and transforming protein that is related to Rho-specific exchange factors and yeast cell cycle regulators. The expression of this gene is elevated with the onset of DNA synthesis and remains elevated during G2 and M phases. In situ hybridization analysis showed that expression is at a high level in cells undergoing mitosis in regenerating liver. Thus, this protein is expressed in a cell cycle-dependent manner during liver regeneration, and is thought to have an important role in the regulation of cytokinesis. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2017] |
Documents
| Product Manuals |
| FAQs |
| SDS |
